| Autor: |
Xing DK; Division of Bacteriology, National Institute for Biological Standards and Control, Hertfordshire, UK. dxing@nibsc.ac.uk, Das RG, Williams L, Canthaboo C, Tremmil J, Corbel MJ |
| Jazyk: |
angličtina |
| Zdroj: |
Vaccine [Vaccine] 1999 Feb 12; Vol. 17 (6), pp. 565-76. |
| DOI: |
10.1016/s0264-410x(98)00235-7 |
| Abstrakt: |
Whole cell and five different types of acellular pertussis vaccine were assayed using a mouse aerosol challenge model which permitted delivery of a controlled, consistent dose of Bordetella pertussis to the lower respiratory tract. Using this system, the viable counts in the lungs of vaccinated mice were immunisation dose-dependent and allowed the protective capacity of different vaccine preparations to be distinguished. This model may thus provide the basis for a protection assay for pertussis vaccines. Comparison of acellular vaccines with a whole cell pertussis vaccine showed that the latter gave better active protection in mice but with a different dose-response relationship. Thus the two types of vaccine are not directly comparable in the same assay and require different reference standards. A pentavalent type acellular vaccine is suggested as a possible candidate standard for the acellular vaccine potency test. The results suggest that this aerosol challenge model has potential for use as a potency test for acellular pertussis vaccines. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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Full Text from ScienceDirect