| Autor: |
Ariese, Freek, Everaarts, Jan M., Boon, Jan P., Stroomberg, Gerard, Holtz, Frank, Meer, Jaap van der, Schanke, Arne van, Berg, Martin van den |
| Předmět: |
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| Zdroj: |
Environmental Toxicology & Chemistry. Aug2001, Vol. 20 Issue 8, p1641. 0p. |
| Abstrakt: |
Polycyclic aromatic hydrocarbons (PAHs) are abundant pollutants, andmany PAHs are carcinogenic, but only after metabolic activation. Benzo[a]pyrene (BaP) is among the most carcinogenic PAHs. The dose and time response of two enzymes involved in BaP metabolism and the amounts of BaP metabolites excreted into the bile were evaluated in an experiment with dab (Limanda limanda). Ninety dab were exposed orally to one of five doses of BaP (0, 0.08, 0.4, 2, or 10 mg/kg) and sampled at 3, 6, or 12 d after exposure. None of the doses studied caused significant induction of either microsomal ethoxyresorufin-O-deethylase (EROD), which reflects cytochrome P450 1A (CYP1A) activity, or cytosolic glutathione-S-transferase activity (GST). Concentrations of biliary BaP metabolites significantly increased with dose and significantlydecreased with time after exposure. It is concluded that biliary BaPmetabolites provide a much more sensitive method than EROD (CYP1A) or GST activity to monitor recent exposure to PAHs in dab. [ABSTRACT FROM AUTHOR] |
| Databáze: |
GreenFILE |
| Externí odkaz: |
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