Polymerase Chain Reaction-Based Sex Identification in the Greater Roadrunner.

Autor: Santamaria, Carlos A.1, Kelley, Samuel2, Schulz, Gerral G.2, Ransom, Jr., Dean2, Hurtado, Luis A.1 lhurtado@tamu.edu
Předmět:
Zdroj: Journal of Wildlife Management. Aug2010, Vol. 74 Issue 6, p1395-1399. 5p. 1 Black and White Photograph, 2 Charts.
Abstrakt: We determined a polymerase chain reaction (PCR)-based method to identify the sex of greater roadrunners (Geococcyx californianus). We found that previously reported primer combinations used in other bird species did not work reliably in roadrunners. However, a novel primer combination (P2-1237L) allowed for consistent and reliable sex identification of roadrunners. Conducting several PCR replicates per sample helped to identify occasional preferential amplification of the Z band that could occur in females (the heterogametic sex; i.e., WZ). Identifying the sex of individuals will allow for examination of sex-associated differences in home range size and habitat use, population demographics, and parenting behavior, as well as for a better understanding of the social system of roadrunners. [ABSTRACT FROM AUTHOR]
Databáze: GreenFILE