Subacute and sublethal ingestion of microcystin-LR impairs lung mitochondrial function by an oligomycin-like effect.

Autor: Mesquita, Flávia Muniz de1 (AUTHOR), de Oliveira, Dahienne Ferreira2 (AUTHOR), Caldeira, Dayene de Assis Fernandes1 (AUTHOR), de Albuquerque, João Paulo Cavalcante1 (AUTHOR), Matta, Leonardo1 (AUTHOR), Faria, Caroline Coelho de1 (AUTHOR), Souza, Itanna Isis Araujo de1 (AUTHOR), Takiya, Christina Maeda1 (AUTHOR), Fortunato, Rodrigo Soares1 (AUTHOR), Nascimento, José Hamilton Matheus1 (AUTHOR), de Oliveira Azevedo, Sandra Maria Feliciano1 (AUTHOR), Zin, Walter Araujo1 (AUTHOR), Maciel, Leonardo1,3 (AUTHOR) leonardomaciel@biof.ufrj.br
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Zdroj: Environmental Toxicology & Pharmacology. Jul2022, Vol. 93, pN.PAG-N.PAG. 1p.
Abstrakt: Microcystin-LR (MC-LR) is a potent cyanotoxin that can reach several organs. However subacute exposure to sublethal doses of MC-LR has not yet well been studied. Herein, we evaluated the outcomes of subacute and sublethal MC-LR exposure on lungs. Male BALB/c mice were exposed to MC-LR by gavage (30 µg/kg) for 20 consecutive days, whereas CTRL mice received filtered water. Respiratory mechanics was not altered in MC-LR group, but histopathology disclosed increased collagen deposition, immunological cell infiltration, and higher percentage of collapsed alveoli. Mitochondrial function was extensively affected in MC-LR animals. Additionally, a direct in vitro titration of MC-LR revealed impaired mitochondrial function. In conclusion, MC-LR presented an intense deleterious effect on lung mitochondrial function and histology. Furthermore, MC-LR seems to exert an oligomycin-like effect in lung mitochondria. This study opens new perspectives for the understanding of the putative pulmonary initial mechanisms of damage resulting from oral MC-LR intoxication. [Display omitted] • MC-LR sublethal and subacute exposure does not impair lung mechanics. • MC-LR sublethal and subacute exposure damages lung mitochondria. • MC-LR (30 nmol/L) can directly impair lung mitochondria. • MC-LR presents an oligomycin-like effect on mitochondria. [ABSTRACT FROM AUTHOR]
Databáze: GreenFILE
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