| Abstrakt: |
In the present study, a sequential approach was applied to optimize the medium in submerged cultivation of Halomonas elongata. Firstly, based on single- factor experiment, the carbon sources, nitrogen sources and inorganic salts were optimizedand shown as follows: L- glutamate sodium 10 g/L, yeast extract powder 14 g/L, NaCl 50 g/L, K2HPO4 0.59 g/L, MnSO4 0.1 g/L, MgSO4 10 g/L, pH 7.0 (Modified MG medium). Afterwards, Halomonas elongata were incubated in 10 L fermenter with 10% inoculum for 25 h (200 rpm, 25 °C). The concentrations of glucose solution and NaCl were maintained at 1±0.5g/L and 15% by supplementing of glucose and solid NaCl since the OD600 reached 90. Ultimately, the ectoine productivy was up to 14.55±1.67 g/L/day, meanwhile, the ratio of hydroxyectoine was less than 0.05%. Additionally, the recycle of the fermentation supernatant was viable and showed no adverse effects on ectoine yield. [ABSTRACT FROM AUTHOR] |
