A turquoise fluorescence lifetime-based biosensor for quantitative imaging of intracellular calcium

Autor: Joachim Goedhart, Jens Puschhof, de Man Sm, Janine J. G. Arts, Anna O. Chertkova, Eike K. Mahlandt, Marten Postma, van Buul Jd, van der Linden Fh, Bas Ponsioen, T. W. J. Gadella, Joep Beumer, Hans Clevers

Publikováno v: Nature Communications, 12(1). Nature Publishing Group
Nature communications, 12(1):7159. Nature Publishing Group
Nature Communications
Nature Communications, 12:7159. Nature Publishing Group
Nature Communications, Vol 12, Iss 1, Pp 1-13 (2021)

The most successful genetically encoded calcium indicators (GECIs) employ an intensity or ratiometric readout. Despite a large calcium-dependent change in fluorescence intensity, the quantification of calcium concentrations with GECIs is problematic,

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::29c7ed39a6bb289f2724218075b9172a
https://pure.knaw.nl/portal/en/publications/3acdad4d-db2c-417e-a263-744ab40a2353

A turquoise fluorescence lifetime-based biosensor for quantitative imaging of intracellular calcium.

Autor: van der Linden FH; Section of Molecular Cytology, van Leeuwenhoek Centre for Advanced Microscopy, Swammerdam Institute for Life Sciences, University of Amsterdam, Amsterdam, The Netherlands., Mahlandt EK; Section of Molecular Cytology, van Leeuwenhoek Centre for Advanced Microscopy, Swammerdam Institute for Life Sciences, University of Amsterdam, Amsterdam, The Netherlands., Arts JJG; Section of Molecular Cytology, van Leeuwenhoek Centre for Advanced Microscopy, Swammerdam Institute for Life Sciences, University of Amsterdam, Amsterdam, The Netherlands.; Department of Molecular Hematology at Sanquin Research and Landsteiner Laboratory, Academic Medical Centre, University of Amsterdam, Amsterdam, The Netherlands., Beumer J; Oncode Institute, Hubrecht Institute, Royal Netherlands Academy of Arts and Sciences and University Medical Center, Utrecht, The Netherlands., Puschhof J; Oncode Institute, Hubrecht Institute, Royal Netherlands Academy of Arts and Sciences and University Medical Center, Utrecht, The Netherlands., de Man SMA; Section of Molecular Cytology, van Leeuwenhoek Centre for Advanced Microscopy, Swammerdam Institute for Life Sciences, University of Amsterdam, Amsterdam, The Netherlands., Chertkova AO; Section of Molecular Cytology, van Leeuwenhoek Centre for Advanced Microscopy, Swammerdam Institute for Life Sciences, University of Amsterdam, Amsterdam, The Netherlands., Ponsioen B; Center for Molecular Medicine, Oncode Institute, University Medical Centre Utrecht, Utrecht, The Netherlands., Clevers H; Oncode Institute, Hubrecht Institute, Royal Netherlands Academy of Arts and Sciences and University Medical Center, Utrecht, The Netherlands., van Buul JD; Section of Molecular Cytology, van Leeuwenhoek Centre for Advanced Microscopy, Swammerdam Institute for Life Sciences, University of Amsterdam, Amsterdam, The Netherlands.; Department of Molecular Hematology at Sanquin Research and Landsteiner Laboratory, Academic Medical Centre, University of Amsterdam, Amsterdam, The Netherlands., Postma M; Section of Molecular Cytology, van Leeuwenhoek Centre for Advanced Microscopy, Swammerdam Institute for Life Sciences, University of Amsterdam, Amsterdam, The Netherlands., Gadella TWJ Jr; Section of Molecular Cytology, van Leeuwenhoek Centre for Advanced Microscopy, Swammerdam Institute for Life Sciences, University of Amsterdam, Amsterdam, The Netherlands., Goedhart J; Section of Molecular Cytology, van Leeuwenhoek Centre for Advanced Microscopy, Swammerdam Institute for Life Sciences, University of Amsterdam, Amsterdam, The Netherlands. j.goedhart@uva.nl.

Publikováno v: Nature communications [Nat Commun] 2021 Dec 09; Vol. 12 (1), pp. 7159. Date of Electronic Publication: 2021 Dec 09.

Bovine natural antibodies in antibody-dependent bactericidal activity against Escherichia coli and Salmonella Typhimurium and risk of mastitis.

Autor: van Altena SE; Cell Biology and Immunology Group, Department of Animal Sciences, Wageningen University, P.O. Box 338, Wageningen, the Netherlands. Electronic address: christine.vanaltena@wur.nl., Peen MA; Cell Biology and Immunology Group, Department of Animal Sciences, Wageningen University, P.O. Box 338, Wageningen, the Netherlands., van der Linden FH; Cell Biology and Immunology Group, Department of Animal Sciences, Wageningen University, P.O. Box 338, Wageningen, the Netherlands., Parmentier HK; Adaptation Physiology Group, Department of Animal Sciences, Wageningen University, P.O. Box 338, Wageningen, the Netherlands., Savelkoul HF; Cell Biology and Immunology Group, Department of Animal Sciences, Wageningen University, P.O. Box 338, Wageningen, the Netherlands., Tijhaar EJ; Cell Biology and Immunology Group, Department of Animal Sciences, Wageningen University, P.O. Box 338, Wageningen, the Netherlands.

Publikováno v: Veterinary immunology and immunopathology [Vet Immunol Immunopathol] 2016 Mar; Vol. 171, pp. 21-7. Date of Electronic Publication: 2016 Feb 04.

Efficient simultaneous excision of multiple selectable marker cassettes using I-SceI-induced double-strand DNA breaks in Saccharomyces cerevisiae.

Autor: Solis-Escalante D; Department of Biotechnology, Delft University of Technology, Delft, The Netherlands., Kuijpers NG, van der Linden FH, Pronk JT, Daran JM, Daran-Lapujade P

Publikováno v: FEMS yeast research [FEMS Yeast Res] 2014 Aug; Vol. 14 (5), pp. 741-54. Date of Electronic Publication: 2014 Jun 27.