MARCH6 and TRC8 facilitate the quality control of cytosolic and tail-anchored proteins

Autor: Stefanovic-Barrett, Sandra, Dickson, Anna S, Burr, Stephen P, Williamson, James C, Lobb, Ian T, Van Den Boomen, Dick Jh, Lehner, Paul J, Nathan, James A

Misfolded or damaged proteins are typically targeted for destruction by proteasome-mediated degradation, but the mammalian ubiquitin machinery involved is incompletely understood. Here, using forward genetic screens in human cells, we find that the p

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::72735d88d08f1ccdb03a5bfbc46d92d3
https://www.repository.cam.ac.uk/handle/1810/275453

The sterol-responsive RNF145 E3 ubiquitin ligase mediates the degradation of HMG-CoA reductase together with gp78 and Hrd1

Autor: Menzies, Sam A, Volkmar, Norbert, Van Den Boomen, Dick Jh, Timms, Richard T, Dickson, Anna S, Nathan, James A, Lehner, Paul J

UNLABELLED: Mammalian HMG-CoA reductase (HMGCR), the rate-limiting enzyme of the cholesterol biosynthetic pathway and the therapeutic target of statins, is post-transcriptionally regulated by sterol-accelerated degradation. Under cholesterol-replete

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::22582d2d75a206dc4bb38dd8c9453b46

A trimeric Rab7 GEF controls NPC1-dependent lysosomal cholesterol export

Autor: Ilana Berlin, Agata Sienkiewicz, Paul J. Lehner, Dick J. H. van den Boomen, Marlieke L.M. Jongsma, Daphne M. van Elsland, J. Paul Luzio, Jacques Neefjes

Publikováno v: Nature Communications
Nature Communications, 11(1). NATURE RESEARCH
Nature Communications, Vol 11, Iss 1, Pp 1-18 (2020)

Cholesterol import in mammalian cells is mediated by the LDL receptor pathway. Here, we perform a genome-wide CRISPR screen using an endogenous cholesterol reporter and identify >100 genes involved in LDL-cholesterol import. We characterise C18orf8 a

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4c2d8cd870120ef7d7703dbb0df2c6f0

Temporal proteomic analysis of HIV infection reveals remodelling of the host phosphoproteome by lentiviral Vif variants.

Autor: Greenwood EJ; Cambridge Institute for Medical Research, Department of Medicine, University of Cambridge, Cambridge, United Kingdom., Matheson NJ; Cambridge Institute for Medical Research, Department of Medicine, University of Cambridge, Cambridge, United Kingdom., Wals K; Cambridge Institute for Medical Research, Department of Medicine, University of Cambridge, Cambridge, United Kingdom., van den Boomen DJ; Cambridge Institute for Medical Research, Department of Medicine, University of Cambridge, Cambridge, United Kingdom., Antrobus R; Cambridge Institute for Medical Research, Department of Medicine, University of Cambridge, Cambridge, United Kingdom., Williamson JC; Cambridge Institute for Medical Research, Department of Medicine, University of Cambridge, Cambridge, United Kingdom., Lehner PJ; Cambridge Institute for Medical Research, Department of Medicine, University of Cambridge, Cambridge, United Kingdom.

Publikováno v: ELife [Elife] 2016 Sep 30; Vol. 5. Date of Electronic Publication: 2016 Sep 30.