Harmonization and standardization of nucleus pulposus cell extraction and culture methods

Autor: Basatvat, Shaghayegh, Bach, Frances C, Barcellona, Marcos N, Binch, Abbie L, Buckley, Conor T, Bueno, Brian, Chahine, Nadeen O, Chee, Ana, Creemers, Laura B, Dudli, Stefan, Fearing, Bailey, Ferguson, Stephen J, Gansau, Jennifer, Gantenbein, Benjamin, Gawri, Rahul, Glaeser, Juliane D, Grad, Sibylle, Guerrero, Julien, Haglund, Lisbet, Hernandez, Paula A, Hoyland, Judith A, Huang, Charles, Iatridis, James C, Illien-Junger, Svenja, Jing, Liufang, Kraus, Petra, Laagland, Lisanne T, Lang, Gernot, Leung, Victor, Li, Zhen, Lufkin, Thomas, van Maanen, Josette C, McDonnell, Emily E, Panebianco, Chris J, Presciutti, Steven M, Rao, Sanjna, Richardson, Stephen M, Romereim, Sarah, Schmitz, Tara C, Schol, Jordy, Setton, Lori, Sheyn, Dmitriy, Snuggs, Joseph W, Sun, Y, Tan, Xiaohong, Tryfonidou, Marianna A, Vo, Nam, Wang, Dong, Williams, Brandon, Williams, Rebecca, Yoon, S Tim, Le Maitre, Christine L

Publikováno v: Basatvat, Shaghayegh; Bach, Frances C; Barcellona, Marcos N; Binch, Abbie L; Buckley, Conor T; Bueno, Brian; Chahine, Nadeen O; Chee, Ana; Creemers, Laura B; Dudli, Stefan; Fearing, Bailey; Ferguson, Stephen J; Gansau, Jennifer; Gantenbein, Benjamin; Gawri, Rahul; Glaeser, Juliane D; Grad, Sibylle; Guerrero, Julien; Haglund, Lisbet; Hernandez, Paula A; ... (2023). Harmonization and standardization of nucleus pulposus cell extraction and culture methods. JOR Spine, 6(1), e1238. Wiley 10.1002/jsp2.1238
JOR Spine, 6(1). Wiley-Blackwell Publishing Ltd
JOR Spine, 6 (1)

Background: In vitro studies using nucleus pulposus (NP) cells are commonly used to investigate disc cell biology and pathogenesis, or to aid in the development of new therapies. However, lab-to-lab variability jeopardizes the much-needed progress in

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https://doi.org/10.1002/jsp2.1238