Human organotypic brain slice cultures: a detailed and improved protocol for preparation and long-term maintenance.

Autor: Bak A; Department of Epileptology, Neurology, RWTH Aachen University Hospital, Aachen, Germany. Electronic address: aniella.va.bak@gmail.com., Koch H; Department of Epileptology, Neurology, RWTH Aachen University Hospital, Aachen, Germany., van Loo KMJ; Department of Epileptology, Neurology, RWTH Aachen University Hospital, Aachen, Germany; Department of Neurosurgery, RWTH Aachen University, Aachen, Germany., Schmied K; Department of Epileptology, Neurology, RWTH Aachen University Hospital, Aachen, Germany., Gittel B; Department of Epileptology, Neurology, RWTH Aachen University Hospital, Aachen, Germany., Weber Y; Department of Epileptology, Neurology, RWTH Aachen University Hospital, Aachen, Germany., Ort J; Department of Neurosurgery, RWTH Aachen University, Aachen, Germany., Schwarz N; Department of Neurology and Epileptology, Hertie Institute for Clinical Brain Research, University of Tübingen, Tübingen, Germany., Tauber SC; Department of Neurology, RWTH Aachen University Hospital, Aachen, Germany., Wuttke TV; Department of Neurology and Epileptology, Hertie Institute for Clinical Brain Research, University of Tübingen, Tübingen, Germany; Department of Neurosurgery, University of Tübingen, Tübingen, Germany., Delev D; Department of Neurosurgery, RWTH Aachen University, Aachen, Germany; Neurosurgical Artificial Intelligence Laboratory Aachen (NAILA), RWTH Aachen University Hospital, Aachen, Germany; Department of Neurosurgery, University of Erlangen, Erlangen, Germany.

Publikováno v: Journal of neuroscience methods [J Neurosci Methods] 2024 Apr; Vol. 404, pp. 110055. Date of Electronic Publication: 2024 Jan 05.

SCN1A overexpression, associated with a genomic region marked by a risk variant for a common epilepsy, raises seizure susceptibility.

Autor: Silvennoinen K; Department of Clinical and Experimental Epilepsy, UCL Queen Square Institute of Neurology, Box 29, Queen Square, London, WC1N 3BG, UK.; Chalfont Centre for Epilepsy, Chalfont St Peter, Bucks, SL9 0RJ, UK., Gawel K; Chemical Neuroscience Group, Centre for Molecular Medicine Norway (NCMM), Faculty of Medicine, University of Oslo, 0349, Oslo, Norway.; Department of Experimental and Clinical Pharmacology, Medical University of Lublin, 20-090, Lublin, Poland., Tsortouktzidis D; Institute of Neuropathology, Medical Faculty, University of Bonn, Section for Translational Epilepsy Research, 53127, Bonn, Germany.; Department of Epileptology, Medical Faculty, University of Bonn, 53127, Bonn, Germany., Pitsch J; Institute of Neuropathology, Medical Faculty, University of Bonn, Section for Translational Epilepsy Research, 53127, Bonn, Germany.; Department of Epileptology, Medical Faculty, University of Bonn, 53127, Bonn, Germany., Alhusaini S; Department of Molecular and Cellular Therapeutics, The Royal College of Surgeons in Ireland, Dublin 2, Ireland.; Department of Neurology, Yale University School of Medicine, New Haven, CT, 06520, USA., van Loo KMJ; Institute of Neuropathology, Medical Faculty, University of Bonn, Section for Translational Epilepsy Research, 53127, Bonn, Germany.; Department of Epileptology and Neurology, RWTH Aachen University, 52074, Aachen, Germany., Picardo R; Department of Neuropathology, UCL Queen Square Institute of Neurology, London, WC1N 3BG, UK., Michalak Z; Department of Neuropathology, UCL Queen Square Institute of Neurology, London, WC1N 3BG, UK., Pagni S; Department of Clinical and Experimental Epilepsy, UCL Queen Square Institute of Neurology, Box 29, Queen Square, London, WC1N 3BG, UK.; Chalfont Centre for Epilepsy, Chalfont St Peter, Bucks, SL9 0RJ, UK., Martins Custodio H; Department of Clinical and Experimental Epilepsy, UCL Queen Square Institute of Neurology, Box 29, Queen Square, London, WC1N 3BG, UK.; Chalfont Centre for Epilepsy, Chalfont St Peter, Bucks, SL9 0RJ, UK., Mills J; Department of Clinical and Experimental Epilepsy, UCL Queen Square Institute of Neurology, Box 29, Queen Square, London, WC1N 3BG, UK.; Chalfont Centre for Epilepsy, Chalfont St Peter, Bucks, SL9 0RJ, UK., Whelan CD; Department of Molecular and Cellular Therapeutics, The Royal College of Surgeons in Ireland, Dublin 2, Ireland.; Imaging Genetics Center, Mark and Mary Stevens Neuroimaging and Informatics Institute, Keck School of Medicine, University of Southern California, Marina del Rey, Los Angele, CA, 90292, USA., de Zubicaray GI; School of Psychology, Faculty of Health, Queensland University of Technology (QUT), Brisbane, QLD, 4059, Australia., McMahon KL; School of Clinical Sciences, Faculty of Health, Queensland University of Technology (QUT), Brisbane, QLD, 4029, Australia., van der Ent W; Chemical Neuroscience Group, Centre for Molecular Medicine Norway (NCMM), Faculty of Medicine, University of Oslo, 0349, Oslo, Norway., Kirstein-Smardzewska KJ; Chemical Neuroscience Group, Centre for Molecular Medicine Norway (NCMM), Faculty of Medicine, University of Oslo, 0349, Oslo, Norway., Tiraboschi E; Chemical Neuroscience Group, Centre for Molecular Medicine Norway (NCMM), Faculty of Medicine, University of Oslo, 0349, Oslo, Norway., Mudge JM; European Molecular Biology Laboratory, European Bioinformatics Institute, Wellcome Genome Campus, Cambridge, CB10 1SD, UK., Frankish A; European Molecular Biology Laboratory, European Bioinformatics Institute, Wellcome Genome Campus, Cambridge, CB10 1SD, UK., Thom M; Department of Neuropathology, UCL Queen Square Institute of Neurology, London, WC1N 3BG, UK., Wright MJ; Queensland Brain Institute, University of Queensland, St Lucia, 4072 QLD, Australia., Thompson PM; Imaging Genetics Center, Mark and Mary Stevens Neuroimaging and Informatics Institute, Keck School of Medicine, University of Southern California, Marina del Rey, Los Angele, CA, 90292, USA., Schoch S; Institute of Neuropathology, Medical Faculty, University of Bonn, Section for Translational Epilepsy Research, 53127, Bonn, Germany.; Department of Epileptology, Medical Faculty, University of Bonn, 53127, Bonn, Germany., Becker AJ; Institute of Neuropathology, Medical Faculty, University of Bonn, Section for Translational Epilepsy Research, 53127, Bonn, Germany., Esguerra CV; Chemical Neuroscience Group, Centre for Molecular Medicine Norway (NCMM), Faculty of Medicine, University of Oslo, 0349, Oslo, Norway., Sisodiya SM; Department of Clinical and Experimental Epilepsy, UCL Queen Square Institute of Neurology, Box 29, Queen Square, London, WC1N 3BG, UK. s.sisodiya@ucl.ac.uk.; Chalfont Centre for Epilepsy, Chalfont St Peter, Bucks, SL9 0RJ, UK. s.sisodiya@ucl.ac.uk.

Publikováno v: Acta neuropathologica [Acta Neuropathol] 2022 Jul; Vol. 144 (1), pp. 107-127. Date of Electronic Publication: 2022 May 12.

Translational imaging of TSPO reveals pronounced innate inflammation in human and murine CD8 T cell-mediated limbic encephalitis.

Autor: Gallus M; Department of Neurology Institute of Translational Neurology, University of Münster, Münster, Germany.; Department of Neurosurgery, University of Münster, Münster, Germany.; Department of Neurosurgery, University of California, San Francisco, San Francisco, CA, USA., Roll W; Department of Nuclear Medicine, University of Münster, Münster, Germany., Dik A; Department of Neurology Institute of Translational Neurology, University of Münster, Münster, Germany.; Department of Neurology, Medical Faculty, Heinrich Heine University of Düsseldorf, Düsseldorf, Germany., Barca C; European Institute for Molecular Imaging, University of Münster, Münster, Germany., Zinnhardt B; European Institute for Molecular Imaging, University of Münster, Münster, Germany.; Biomarkers and Translational Technologies (BTT), Pharma Research and Early Development (pRED), F. Hoffmann-La Roche Ltd., Basel, Switzerland., Hicking G; Department of Neurology Institute of Translational Neurology, University of Münster, Münster, Germany., Mueller C; Department of Neurology Institute of Translational Neurology, University of Münster, Münster, Germany., Naik VN; Department of Neurology Institute of Translational Neurology, University of Münster, Münster, Germany., Anstötz M; Institute of Anatomy II, Medical Faculty, University Hospital Düsseldorf, Heinrich-Heine-University, Düsseldorf, Germany., Krämer J; Department of Neurology Institute of Translational Neurology, University of Münster, Münster, Germany., Rolfes L; Department of Neurology Institute of Translational Neurology, University of Münster, Münster, Germany.; Department of Neurology, Medical Faculty, Heinrich Heine University of Düsseldorf, Düsseldorf, Germany., Wachsmuth L; Department of Clinical Radiology, University of Münster, Münster, Germany., Pitsch J; Department of Epileptology, University of Bonn, Bonn, Germany., van Loo KMJ; Section for Translational Epilepsy Research, Department of Neuropathology, University of Bonn, Bonn, Germany.; Department of Epileptology and Neurology, RWTH Aachen University, Aachen, Germany., Räuber S; Department of Neurology Institute of Translational Neurology, University of Münster, Münster, Germany.; Department of Neurology, Medical Faculty, Heinrich Heine University of Düsseldorf, Düsseldorf, Germany., Okada H; Department of Neurosurgery, University of California, San Francisco, San Francisco, CA, USA., Wimberley C; Edinburgh Imaging, University of Edinburgh, Edinburgh, UK., Strippel C; Department of Neurology Institute of Translational Neurology, University of Münster, Münster, Germany., Golombeck KS; Department of Neurology Institute of Translational Neurology, University of Münster, Münster, Germany.; Department of Neurology, Medical Faculty, Heinrich Heine University of Düsseldorf, Düsseldorf, Germany., Johnen A; Department of Neurology Institute of Translational Neurology, University of Münster, Münster, Germany., Kovac S; Department of Neurology Institute of Translational Neurology, University of Münster, Münster, Germany., Groß CC; Department of Neurology Institute of Translational Neurology, University of Münster, Münster, Germany., Backhaus P; Department of Nuclear Medicine, University of Münster, Münster, Germany.; European Institute for Molecular Imaging, University of Münster, Münster, Germany., Seifert R; Department of Nuclear Medicine, University of Münster, Münster, Germany.; European Institute for Molecular Imaging, University of Münster, Münster, Germany., Lewerenz J; Department of Neurology, University of Ulm, Ulm, Germany., Surges R; Department of Epileptology, University of Bonn, Bonn, Germany., Elger CE; Department of Epileptology, University of Bonn, Bonn, Germany., Wiendl H; Department of Neurology Institute of Translational Neurology, University of Münster, Münster, Germany., Ruck T; Department of Neurology Institute of Translational Neurology, University of Münster, Münster, Germany.; Department of Neurology, Medical Faculty, Heinrich Heine University of Düsseldorf, Düsseldorf, Germany., Becker AJ; Section for Translational Epilepsy Research, Department of Neuropathology, University of Bonn, Bonn, Germany., Faber C; Department of Clinical Radiology, University of Münster, Münster, Germany., Jacobs AH; European Institute for Molecular Imaging, University of Münster, Münster, Germany., Bauer J; Department of Neuroimmunology, Centre for Brain Research, Medical University of Vienna, Vienna, Austria., Meuth SG; Department of Neurology Institute of Translational Neurology, University of Münster, Münster, Germany.; Department of Neurology, Medical Faculty, Heinrich Heine University of Düsseldorf, Düsseldorf, Germany., Schäfers M; Department of Nuclear Medicine, University of Münster, Münster, Germany.; European Institute for Molecular Imaging, University of Münster, Münster, Germany., Melzer N; Department of Neurology Institute of Translational Neurology, University of Münster, Münster, Germany.; Department of Neurology, Medical Faculty, Heinrich Heine University of Düsseldorf, Düsseldorf, Germany.

Publikováno v: Science advances [Sci Adv] 2023 Jun 09; Vol. 9 (23), pp. eabq7595. Date of Electronic Publication: 2023 Jun 09.

Neurochemical Profile of BRAFV600E/AktT308D/S473D Mouse Gangliogliomas Reveals Impaired GABAergic System Inhibition.

Autor: Kyriazi M; Institute of Neuropathology, Section for Translational Epilepsy Research, Medical Faculty, University of Bonn, Bonn, Germany., Müller P; Institute of Neuropathology, Section for Translational Epilepsy Research, Medical Faculty, University of Bonn, Bonn, Germany., Pitsch J; Department of Epileptology, Medical Faculty, University of Bonn, Bonn, Germany., van Loo KMJ; Department of Epileptology, Neurology, RWTH Aachen University, Aachen, Germany., Quatraccioni A; Institute of Neuropathology, Section for Translational Epilepsy Research, Medical Faculty, University of Bonn, Bonn, Germany., Opitz T; Institute of Experimental Epileptology and Cognition Research, Medical Faculty, University of Bonn, Bonn, Germany., Schoch S; Institute of Neuropathology, Section for Translational Epilepsy Research, Medical Faculty, University of Bonn, Bonn, Germany.; Department of Epileptology, Medical Faculty, University of Bonn, Bonn, Germany., Becker AJ; Institute of Neuropathology, Section for Translational Epilepsy Research, Medical Faculty, University of Bonn, Bonn, Germany., Cases-Cunillera S; Institute of Neuropathology, Section for Translational Epilepsy Research, Medical Faculty, University of Bonn, Bonn, Germany.

Publikováno v: Developmental neuroscience [Dev Neurosci] 2023; Vol. 45 (2), pp. 53-65. Date of Electronic Publication: 2022 Dec 20.

Heterogeneity and excitability of BRAFV600E-induced tumors is determined by Akt/mTOR-signaling state and Trp53-loss.

Autor: Cases-Cunillera S; Institute of Neuropathology, Section for Translational Epilepsy Research, Medical Faculty, University of Bonn, Bonn, Germany., van Loo KMJ; Institute of Neuropathology, Section for Translational Epilepsy Research, Medical Faculty, University of Bonn, Bonn, Germany.; Department of Epileptology, Neurology, RWTH Aachen University, Aachen, Germany., Pitsch J; Department of Epileptology, Medical Faculty, University of Bonn, Bonn, Germany., Quatraccioni A; Institute of Neuropathology, Section for Translational Epilepsy Research, Medical Faculty, University of Bonn, Bonn, Germany., Sivalingam S; Institute of Neuropathology, Section for Translational Epilepsy Research, Medical Faculty, University of Bonn, Bonn, Germany.; Department of Human Genetics, Medical Faculty, University of Bonn, Bonn, Germany., Salomoni P; Nuclear Function Group, German Centre for Neurodegenerative Diseases (DZNE), Bonn, Germany., Borger V; Department of Neurosurgery, University Hospital Bonn, Bonn, Germany., Dietrich D; Department of Neurosurgery, University Hospital Bonn, Bonn, Germany., Schoch S; Department of Epileptology, Medical Faculty, University of Bonn, Bonn, Germany., Becker AJ; Institute of Neuropathology, Section for Translational Epilepsy Research, Medical Faculty, University of Bonn, Bonn, Germany.

Publikováno v: Neuro-oncology [Neuro Oncol] 2022 May 04; Vol. 24 (5), pp. 741-754.