Cas9 RNP transfection by vapor nanobubble photoporation for ex vivo cell engineering

Autor: Raes, Laurens, Pille, Melissa, Harizaj, Aranit, Goetgeluk, Glenn, van Hoeck, Jelter, Stremersch, Stephan, Fraire, Juan C, Brans, Toon, de Jong, Olivier G, Maas - Bakker, Roel, Mastrobattista, Enrico, Vader, Pieter, de Smedt, Stefaan C, Vandekerckhove, Bart, Raemdonck, Koen, Braeckmans, Kevin, Afd Pharmaceutics, Pharmaceutics

Publikováno v: MOLECULAR THERAPY-NUCLEIC ACIDS
Molecular Therapy-Nucleic Acids, 25, 696. Cell Press
Molecular Therapy: Nucleic Acids, Vol 25, Iss, Pp 696-707 (2021)

The CRISPR-Cas9 technology represents a powerful tool for genome engineering in eukaryotic cells, advancing both fundamental research and therapeutic strategies. Despite the enormous potential of the technology, efficient and direct intracellular del

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::39b7f8e5430f30f764d9ff5200f4d081
https://doi.org/10.1016/j.omtn.2021.08.014

Hydrogel-Induced Cell Membrane Disruptions Enable Direct Cytosolic Delivery of Membrane-Impermeable Cargo

Autor: Van Hoeck, Jelter, Van de Vyver, Thijs, Harizaj, Aranit, Goetgeluk, Glenn, Merckx, Pieterjan, Liu, Jing, Wels, Mike, Sauvage, Félix, De Keersmaecker, Herlinde, Vanhove, Christian, Vader, Pieter, de Jong, Olivier G., Dewitte, Heleen, Vandekerckhove, Bart, Braeckmans, Kevin, de Smedt, S.C., Raemdonck, Koen

Intracellular delivery of membrane-impermeable cargo offers unique opportunities for biological research and the development of cell-based therapies. Despite the breadth of available intracellular delivery tools, existing protocols are often suboptim

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=od_______101::3a367c673c9fc0a61440e3d8391c3781
https://dspace.library.uu.nl/handle/1874/412767

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Hydrogel-Induced Cell Membrane Disruptions Enable Direct Cytosolic Delivery of Membrane-Impermeable Cargo.

Autor: Van Hoeck J; Ghent Research Group on Nanomedicines, Laboratory of General Biochemistry and Physical Pharmacy, Faculty of Pharmaceutical Sciences, Ghent University, Ottergemsesteenweg 460, Ghent, 9000, Belgium., Van de Vyver T; Ghent Research Group on Nanomedicines, Laboratory of General Biochemistry and Physical Pharmacy, Faculty of Pharmaceutical Sciences, Ghent University, Ottergemsesteenweg 460, Ghent, 9000, Belgium., Harizaj A; Ghent Research Group on Nanomedicines, Laboratory of General Biochemistry and Physical Pharmacy, Faculty of Pharmaceutical Sciences, Ghent University, Ottergemsesteenweg 460, Ghent, 9000, Belgium., Goetgeluk G; Department of Diagnostic Sciences, Ghent University, Corneel Heymanslaan 10, Ghent, 9000, Belgium., Merckx P; Ghent Research Group on Nanomedicines, Laboratory of General Biochemistry and Physical Pharmacy, Faculty of Pharmaceutical Sciences, Ghent University, Ottergemsesteenweg 460, Ghent, 9000, Belgium., Liu J; Ghent Research Group on Nanomedicines, Laboratory of General Biochemistry and Physical Pharmacy, Faculty of Pharmaceutical Sciences, Ghent University, Ottergemsesteenweg 460, Ghent, 9000, Belgium., Wels M; Ghent Research Group on Nanomedicines, Laboratory of General Biochemistry and Physical Pharmacy, Faculty of Pharmaceutical Sciences, Ghent University, Ottergemsesteenweg 460, Ghent, 9000, Belgium., Sauvage F; Ghent Research Group on Nanomedicines, Laboratory of General Biochemistry and Physical Pharmacy, Faculty of Pharmaceutical Sciences, Ghent University, Ottergemsesteenweg 460, Ghent, 9000, Belgium., De Keersmaecker H; Ghent Research Group on Nanomedicines, Laboratory of General Biochemistry and Physical Pharmacy, Faculty of Pharmaceutical Sciences, Ghent University, Ottergemsesteenweg 460, Ghent, 9000, Belgium.; Centre for Advanced Light Microscopy, Ghent University, Ottergemsesteenweg 460, Ghent, 9000, Belgium., Vanhove C; Infinity Lab, Medical Imaging and Signal Processing Group-IBiTech, Faculty of Engineering and Architecture, Ghent University, Corneel Heymanslaan 10, Ghent, 9000, Belgium., de Jong OG; CDL Research, Division LAB, UMC Utrecht, Faculty of Medicine, Utrecht University, Heidelberglaan 100, Utrecht, 3584 CX, The Netherlands.; Department of Pharmaceutics, Utrecht Institute for Pharmaceutical Sciences (UIPS), Faculty of Science, Utrecht University, Universiteitsweg 99, Utrecht, 3584 CG, The Netherlands., Vader P; CDL Research, Division LAB, UMC Utrecht, Faculty of Medicine, Utrecht University, Heidelberglaan 100, Utrecht, 3584 CX, The Netherlands., Dewitte H; Ghent Research Group on Nanomedicines, Laboratory of General Biochemistry and Physical Pharmacy, Faculty of Pharmaceutical Sciences, Ghent University, Ottergemsesteenweg 460, Ghent, 9000, Belgium., Vandekerckhove B; Department of Diagnostic Sciences, Ghent University, Corneel Heymanslaan 10, Ghent, 9000, Belgium., Braeckmans K; Ghent Research Group on Nanomedicines, Laboratory of General Biochemistry and Physical Pharmacy, Faculty of Pharmaceutical Sciences, Ghent University, Ottergemsesteenweg 460, Ghent, 9000, Belgium.; Centre for Advanced Light Microscopy, Ghent University, Ottergemsesteenweg 460, Ghent, 9000, Belgium., De Smedt SC; Ghent Research Group on Nanomedicines, Laboratory of General Biochemistry and Physical Pharmacy, Faculty of Pharmaceutical Sciences, Ghent University, Ottergemsesteenweg 460, Ghent, 9000, Belgium.; Centre for Advanced Light Microscopy, Ghent University, Ottergemsesteenweg 460, Ghent, 9000, Belgium., Raemdonck K; Ghent Research Group on Nanomedicines, Laboratory of General Biochemistry and Physical Pharmacy, Faculty of Pharmaceutical Sciences, Ghent University, Ottergemsesteenweg 460, Ghent, 9000, Belgium.

Publikováno v: Advanced materials (Deerfield Beach, Fla.) [Adv Mater] 2021 Jul; Vol. 33 (30), pp. e2008054. Date of Electronic Publication: 2021 Jun 09.