NADPH biosensor-based identification of an alcohol dehydrogenase variant with improved catalytic properties caused by a single charge reversal at the protein surface

Autor: Spielmann, Alina, Brack, Yannik, van Beek, Hugo, Flachbart, Lion, Sundermeyer, Lea, Baumgart, Meike, Bott, Michael

Publikováno v: AMB Express, Vol 10, Iss 1, Pp 1-12 (2020)
AMB Express
AMB express 10(1), 14 (2020). doi:10.1186/s13568-020-0946-7

Alcohol dehydrogenases (ADHs) are used in reductive biotransformations for the production of valuable chiral alcohols. In this study, we used a high-throughput screening approach based on the NADPH biosensor pSenSox and fluorescence-activated cell so

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid_dedup__::594747c7b55d32520df074d235f8de3f
https://doaj.org/article/e074c063386f425497441301fd682fa6

Engineering and application of a biosensor with focused ligand specificity

Autor: Della Corte, Dennis, van Beek, Hugo, Morris, Connor J., Grünberger, Alexander, Hofmann, Eckhard, Schröder, Gunnar F., Marienhagen, Jan, Syberg, Falk, Schallmey, Marcus, Tobola, Felix, Cormann, Kai, Schlicker, Christine, Baumann, Philipp Tobias, Krumbach, Karin, Sokolowsky, Sascha

Publikováno v: Nature Communications, Vol 11, Iss 1, Pp 1-11 (2020)
Nature Communications 11, 4851 (2020). doi:10.1038/s41467-020-18400-0
'Nature Communications ', vol: 11, pages: 4851-1-4851-11 (2020)
Nature Communications
Nature Communications 11(1), 4851 (2020). doi:10.1038/s41467-020-18400-0

Cell factories converting bio-based precursors to chemicals present an attractive avenue to a sustainable economy, yet screening of genetically diverse strain libraries to identify the best-performing whole-cell biocatalysts is a low-throughput endea

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid_dedup__::de00430998fecf6c8882595cfe8ea8da
https://pub.uni-bielefeld.de/record/2946333

Lyophilization conditions for the storage of monooxygenases

Autor: van Beek, Hugo L., Beyer, Nina, Janssen, Dick B., Fraaije, Marco W.

Publikováno v: In Journal of Biotechnology 10 June 2015 203:41-44

Expanding the applicability of Baeyer-Villiger Monooxygenases

Autor: van Beek, Hugo

In het proefschrift “Expanding the applicability of Baeyer-Villiger monooxygenases” wordt op verschillende manieren het gebruik van deze klasse van enzymen gemakkelijker gemaakt. Enzymen worden in toenemende mate gebruikt om puur chemische proces

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=narcis______::ece692b61be045d8ca779a194d7d4a71
https://research.rug.nl/en/publications/37a25e07-834d-44ed-96b1-48b0360e86a7

Exploring the Structural Basis of Substrate Preferences in Baeyer-Villiger Monooxygenases INSIGHT FROM STEROID MONOOXYGENASE

Autor: Franceschini, Stefano, van Beek, Hugo L., Pennetta, Alessandra, Martinoli, Christian, Fraaije, Marco W., Mattevi, Andrea

Publikováno v: The Journal of Biological Chemistry, 287(27), 22626-22634. AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC

Steroid monooxygenase (STMO) from Rhodococcus rhodochrous catalyzes the Baeyer-Villiger conversion of progesterone into progesterone acetate using FAD as prosthetic group and NADPHas reducing cofactor. The enzyme shares high sequence similarity with

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=narcis______::d9e2b7f59730ab15e7fdedb2f55e61e8
https://research.rug.nl/en/publications/a84ca3d8-a252-43a4-b02c-26f4051ae718