Výsledky vyhledávání - "universal reporter"

Akademický článek

Simplified development of multiplex real-time PCR through master mix augmented by universal fluorogenic reporters

Autor: Simon Wadle, Michael Lehnert, Friedrich Schuler, René Köppel, Annerose Serr, Roland Zengerle, Felix von Stetten

Publikováno v: BioTechniques, Vol 61, Iss 3, Pp 123-128 (2016)

Mediator probe (MP) PCR is a real-time PCR approach that uses standardized universal fluorogenic reporter oligonucleotides (UR) in conjunction with label-free sequence-specific probes. To enable multiplex real-time MP PCR, we designed a set of five o

Externí odkaz: https://doaj.org/article/f4d9af57eaa7470fabf45325be843c3f

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Stringent Base Specific and Optimization-Free Multiplex Mediator Probe ddPCR for the Quantification of Point Mutations in Circulating Tumor DNA

Autor: Inga Höffkes, Max Deuter, Felix von Stetten, Nikolas von Bubnoff, Michael Lehnert, Julius Wehrle, Florian Scherer, Peter Juelg, Nadine Borst, Tobias Hutzenlaub, Roland Zengerle, Elena Kipf, Franziska Schlenker

Publikováno v: Cancers, Vol 13, Iss 5742, p 5742 (2021)
Cancers
Volume 13
Issue 22

Simple Summary Cancer treatment strategies and their follow-up monitoring are changing to personalized therapies, based on molecular genetic information from the individual person. Liquid biopsy, where this molecular information is derived from body

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4a4cfd095d9d1640707d24e110ca9e04
https://www.mdpi.com/2072-6694/13/22/5742

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Akademický článek

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Duplex microRNAs assay based on target-triggered universal reporter hybridization

Autor: Yue Sun, Yinan Wang, Jianzhong Lu, Choiwan Lau

Publikováno v: Journal of Pharmaceutical Analysis
Journal of Pharmaceutical Analysis, Vol 8, Iss 4, Pp 265-270 (2018)

In this paper, we designed and evaluated a duplex detection strategy for microRNAs (miRNAs) using universal probe-based target-triggered double hybridization and fluorescent microsphere-based assay system (xMAP array). In the absence of target miRNA,

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1bf9a4020ec8abfe39a1b52701082dfe
https://doi.org/10.1016/j.jpha.2018.07.004

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Akademický článek

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Real-time PCR probe optimization using design of experiments approach

Autor: Roland Zengerle, F. von Stetten, Simon Wadle, Michael Lehnert, Stefanie Rubenwolf

Publikováno v: Biomolecular Detection and Quantification
Biomolecular Detection and Quantification, Vol 7, Iss C, Pp 1-8 (2016)

Graphical abstract
Highlights • Design of experiments (DOE) is an appropriate approach for probe sequence optimization in real-time PCR. • Nine MP designs led to maximum information for the 3 input factors. • Using DOE the maximum number o

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ca554001d0a01039fba170f041f078ca
http://europepmc.org/articles/PMC4827641

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A Split-Luciferase Reporter Recognizing GFP and mCherry Tags to Facilitate Studies of Protein–Protein Interactions

Autor: Yann Gambin, Emma Sierecki, Mehdi Moustaqil, Laura Gonzalez, Dominic J. B. Hunter, Lisa Raoul, Pascal Carrive, Akshay Bhumkar

Publikováno v: International Journal of Molecular Sciences
International Journal of Molecular Sciences; Volume 18; Issue 12; Pages: 2681
International Journal of Molecular Sciences, Vol 18, Iss 12, p 2681 (2017)

The use of fluorescently-tagged proteins in microscopy has become routine, and anti-GFP (Green fluorescent protein) affinity matrices are increasingly used in proteomics protocols. However, some protein–protein interactions assays, such as protein

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::33285f5415ffd6cb9ab95c267f9a4793
http://europepmc.org/articles/PMC5751283

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