Translation Elongation Factor EF-Tu Modulates Filament Formation of Actin-Like MreB Protein In Vitro

Autor: Peter L. Graumann, Christian Reimold, Hervé Joël Defeu Soufo, Hannes Breddermann, Hans Georg Mannherz

Publikováno v: Journal of Molecular Biology. 427(8):1715-1727

EF-Tu has been shown to interact with actin-like protein MreB and to affect its localization in Escherichia coli and in Bacillus subtilis cells. We have purified YFP-MreB in an active form, which forms filaments on glass slides in vitro and was activ

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Translation elongation factor EF-Tu modulates filament formation of actin-like MreB protein in vitro.

Autor: Defeu Soufo HJ; Microbiology, Faculty of Biology, University of Freiburg, Schänzlestrasse 1, 79104 Freiburg, Germany. Electronic address: joel.defeusoufo@biologie.uni-freiburg.de., Reimold C; LOEWE Center for Synthetic Microbiology (SYNMIKRO) and Department of Chemistry, Philipps Universität, 35037 Marburg, Germany., Breddermann H; LOEWE Center for Synthetic Microbiology (SYNMIKRO) and Department of Chemistry, Philipps Universität, 35037 Marburg, Germany., Mannherz HG; Abteilung für Anatomie und Molekulare Embryologie, Ruhr-Universität, 44801 Bochum, Germany., Graumann PL; LOEWE Center for Synthetic Microbiology (SYNMIKRO) and Department of Chemistry, Philipps Universität, 35037 Marburg, Germany. Electronic address: peter.graumann@synmikro.uni-marburg.de.

Publikováno v: Journal of molecular biology [J Mol Biol] 2015 Apr 24; Vol. 427 (8), pp. 1715-27. Date of Electronic Publication: 2015 Feb 10.