Modulation of protein-DNA binding reveals mechanisms of spatiotemporal gene control in early Drosophila embryos

Autor: Sahla Syed, Yifei Duan, Bomyi Lim

Publikováno v: eLife, Vol 12 (2023)

It is well known that enhancers regulate the spatiotemporal expression of their target genes by recruiting transcription factors (TFs) to the cognate binding sites in the region. However, the role of multiple binding sites for the same TFs and their

Externí odkaz: https://doaj.org/article/f871881b1f6844159f679c7137604c72

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High-throughput single-molecule experiments reveal heterogeneity, state switching, and three interconnected pause states in transcription

Autor: Richard Janissen, Behrouz Eslami-Mossallam, Irina Artsimovitch, Martin Depken, Nynke H. Dekker

Publikováno v: Cell Reports, 39(4)

Pausing by bacterial RNA polymerase (RNAp) is vital in the recruitment of regulatory factors, RNA folding, and coupled translation. While backtracking and intra-structural isomerization have been proposed to trigger pausing, our mechanistic understan

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::50115ecc6840d564199ed3f365cea03a
https://pubmed.ncbi.nlm.nih.gov/35476989

Transcription activation is enhanced by multivalent interactions independent of phase separation

Autor: Jorge Trojanowski, Lukas Frank, Anne Rademacher, Norbert Mücke, Pranas Grigaitis, Karsten Rippe

Publikováno v: Molecular cell, 82(10), 1878-1893.e10. Cell Press
Trojanowski, J, Frank, L, Rademacher, A, Mücke, N, Grigaitis, P & Rippe, K 2022, ' Transcription activation is enhanced by multivalent interactions independent of phase separation ', Molecular cell, vol. 82, no. 10, pp. 1878-1893.e10 . https://doi.org/10.1016/j.molcel.2022.04.017

Transcription factors (TFs) consist of a DNA-binding domain and an activation domain (AD) that are frequently considered to be independent and exchangeable modules. However, recent studies report that the physicochemical properties of the AD can cont

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::49a49d534174115399522cdee458879f
https://doi.org/10.1016/j.molcel.2022.04.017

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