Zobrazeno 1 - 10
of 132
pro vyhledávání: '"transcript quantification"'
Autor:
Guo-Wei Li, Fang Nan, Guo-Hua Yuan, Chu-Xiao Liu, Xindong Liu, Ling-Ling Chen, Bin Tian, Li Yang
Publikováno v:
Genome Biology, Vol 22, Iss 1, Pp 1-24 (2021)
Abstract Single-cell RNA-seq (scRNA-seq) profiles gene expression with high resolution. Here, we develop a stepwise computational method-called SCAPTURE to identify, evaluate, and quantify cleavage and polyadenylation sites (PASs) from 3′ tag-based
Externí odkaz:
https://doaj.org/article/f3a3c7780e4e44299ca918ddc95afacf
Publikováno v:
Algorithms for Molecular Biology, Vol 16, Iss 1, Pp 1-15 (2021)
Abstract Background The probability of sequencing a set of RNA-seq reads can be directly modeled using the abundances of splice junctions in splice graphs instead of the abundances of a list of transcripts. We call this model graph quantification, wh
Externí odkaz:
https://doaj.org/article/3aa32f8e04cf4d65a2f332686ae4d0ae
Akademický článek
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Publikováno v:
Biomolecules, Vol 10, Iss 6, p 827 (2020)
Alternative promoter usage involved in the regulation of transcription, splicing, and translation contributes to proteome diversity and is involved in a large number of diseases, in particular, cancer. Epigenetic mechanisms and cis regulatory element
Externí odkaz:
https://doaj.org/article/b63be59ed2f042b28a9ed206bb9cafcc
Akademický článek
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Autor:
Randall Jeffrey Platt, Mariia Y. Cherepkova, Michal J. Okoniewski, Florian I. Schmidt, Tanmay Tanna
Publikováno v:
Nature Protocols
Nature Protocols, 15
Nature Protocols, 15
Nature Protocols, 15
ISSN:1750-2799
ISSN:1745-2481
ISSN:1754-2189
ISSN:1750-2799
ISSN:1745-2481
ISSN:1754-2189
Publikováno v:
Digital.CSIC. Repositorio Institucional del CSIC
instname
RNA Biol
instname
RNA Biol
miRNAs are small RNAs that regulate mRNAs at both transcriptional and posttranscriptional level. In plants, miRNAs are involved in the regulation of different processes including development and stress-response. Elucidating how stress-responsive miRN
Gene expression studies using chimeric xenograft transplants or co-culture systems have proven to be valuable to uncover cellular dynamics and interactions during development or in disease models. However, the mRNA sequence similarities among species
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::60325ee4267f4adbfc51cc5b795f38eb
https://doi.org/10.1101/2021.06.09.447735
https://doi.org/10.1101/2021.06.09.447735
Autor:
David P. Olson, David Garcia-Galiano, Martin G. Myers, Suzanne M. Moenter, Carol F. Elias, Xingfa Han, Laura L. Burger
Publikováno v:
STAR Protocols
STAR Protocols, Vol 2, Iss 2, Pp 100589-(2021)
STAR Protocols, Vol 2, Iss 2, Pp 100589-(2021)
Summary Here, we present an in-depth protocol for extracting ribosome-bound mRNAs in low-abundance cells of hypothalamic nuclei. mRNAs are extracted from the micropunched tissue using refined translating ribosome affinity purification. Isolated RNAs
Publikováno v:
Cells
Volume 10
Issue 2
Volume 10
Issue 2
Uncovering the physiological role of individual proteins that are part of the intricate process of cellular signaling is often a complex and challenging task. A straightforward strategy of studying a protein’s function is by manipulating the expres