An Accurate, Rapid and Cost-Effective Method for T-nos Detection Based on CRISPR/Cas12a

Autor: Yuling Wang, Cheng Peng, Lin Ding, Zhixun Su, Xiaoyun Chen, Xiaofu Wang, Meihao Sun, Junfeng Xu

Publikováno v: Foods, Vol 12, Iss 3, p 615 (2023)

CRISPR/Cas12a technology is used for nucleic acid detection due to its specific recognition function and non-specific single-stranded DNA cleavage activity. Here, we developed a fluorescence visualisation detection method based on PCR and CRISPR/Cas1

Externí odkaz: https://doaj.org/article/38d5f426f0774f51b4506a56bf290b54

Development of an efficient element-specific PCR method for the detection of genetically modified rice, soy & corn

Autor: Zahra Noormohammadi, Hamid-Reza Zamanizadeh, Neda Sepahiann, Masoud Sheidai

Publikováno v: Genetika, Vol 51, Iss 3, Pp 895-905 (2019)

With the ever-increasing number of GM crops introduced to the market, it is required to respect customers’ freedom of choice and their perception toward GMOs. According to obligatory demand by food and drug administration for GM testing on raw and

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::06207a04c8e7427d2f5e9bcf74fba075
http://www.doiserbia.nb.rs/img/doi/0534-0012/2019/0534-00121903895S.pdf

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Recombinase Polymerase Amplification (RPA) of CaMV-35S Promoter and nos Terminator for Rapid Detection of Genetically Modified Crops

Autor: Xu Chao, Li Liang, Wujun Jin, Yusong Wan

Publikováno v: International Journal of Molecular Sciences, Vol 15, Iss 10, Pp 18197-18205 (2014)
International Journal of Molecular Sciences
Volume 15
Issue 10
Pages 18197-18205

Recombinase polymerase amplification (RPA) is a novel isothermal DNA amplification and detection technology that enables the amplification of DNA within 30 min at a constant temperature of 37–42 °C by simulating in vivo DNA recombination. In this

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::089ec9f41802a407e8fc164dbe73376b
http://www.mdpi.com/1422-0067/15/10/18197

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