A New Support Film for Cryo Electron Microscopy Protein Structure Analysis Based on Covalently Functionalized Graphene

Autor: Philip Nickl, Tarek Hilal, Daniel Olal, Ievgen Sergeevitch Donskyi, Jörg Radnik, Kai Ludwig, Rainer Haag

Protein adsorption at the air–water interface is a serious problem in cryogenic electron microscopy (cryoEM) as it restricts particle orientations in the vitrified ice-film and promotes protein denaturation. To address this issue, the preparation o

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7d7f9024bc78e6db5eda5b3ea7968a15

PROTEINS, INTERFACES, AND CRYO-EM GRIDS

Autor: Robert M. Glaeser

Publikováno v: Glaeser, RM. (2018). Proteins, interfaces, and cryo-EM grids. Current Opinion in Colloid and Interface Science, 34, 1-8. doi: 10.1016/j.cocis.2017.12.009. Lawrence Berkeley National Laboratory: Retrieved from: http://www.escholarship.org/uc/item/4b23r25h

© 2017 Elsevier Ltd It has become clear that the standard cartoon, in which macromolecular particles prepared for electron cryo-microscopy are shown to be surrounded completely by vitreous ice, often is not accurate. In particular, the standard pict

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https://pubmed.ncbi.nlm.nih.gov/29867291

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Electron microscopy of biological macromolecules: Bridging the gap between what physics allows and what we currently can get

Autor: Kenneth H. Downing, Dieter Typke, Robert M. Glaeser

Publikováno v: Typke, Dieter; Downing, Kenneth H.; & Glaeser, Robert M.(2003). Electron microscopy of biological macromolecules: Bridging the gap between what physics allows and what we currently can get. Lawrence Berkeley National Laboratory. Lawrence Berkeley National Laboratory: Lawrence Berkeley National Laboratory. Retrieved from: http://www.escholarship.org/uc/item/86m4b108

The resolution achieved in low-dose electron microscopy of biological macromolecules is significantly worse than what can be obtained on the same microscopes with more robust specimens. When two-dimensional crystals are used, it is also apparent that

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http://www.escholarship.org/uc/item/86m4b108

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