Zobrazeno 1 - 10
of 95
pro vyhledávání: '"sulfur-SAD"'
Publikováno v:
Current Research in Structural Biology, Vol 3, Iss , Pp 112-120 (2021)
Single-wavelength anomalous dispersion (SAD)-phasing using sulfur as the unique anomalous scatterer is a powerful method to solve the phase problem in protein crystallography. However, it is not yet widely used by non-expert crystallographers. We rep
Externí odkaz:
https://doaj.org/article/720751bbf22448928042bc18c0543906
Autor:
Sanchari Banerjee, Nathan P. Coussens, François-Xavier Gallat, Nitish Sathyanarayanan, Jandhyam Srikanth, Koichiro J. Yagi, James S. S. Gray, Stephen S. Tobe, Barbara Stay, Leonard M. G. Chavas, Subramanian Ramaswamy
Publikováno v:
IUCrJ, Vol 3, Iss 4, Pp 282-293 (2016)
Macromolecular crystals for X-ray diffraction studies are typically grown in vitro from pure and homogeneous samples; however, there are examples of protein crystals that have been identified in vivo. Recent developments in micro-crystallography tech
Externí odkaz:
https://doaj.org/article/ebe3dc4f3ca84b0d8f0eee4a8f5e516f
Publikováno v:
IUCrJ, Vol 2, Iss 4, Pp 431-440 (2015)
Native SAD phasing uses the anomalous scattering signal of light atoms in the crystalline, native samples of macromolecules collected from single-wavelength X-ray diffraction experiments. These atoms include sodium, magnesium, phosphorus, sulfur, chl
Externí odkaz:
https://doaj.org/article/9100ca20118e408cb21bfc4390f66a20
Akademický článek
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Autor:
Jens Erik Nielsen, Herman S. Overkleeft, Kristian B. R. M. Krogh, Keith A. Stubbs, Gideon J. Davies, Marta Artola, Johan P. Turkenburg, Nicholas McGregor
Publikováno v:
Acta Crystallografica Section D. Structural Biology, 76(11), 1124-1133
Acta Crystallographica. Section D, Structural Biology
Acta Crystallographica. Section D, Structural Biology
The structure of a fungal α-l-arabinofuranosidase has been determined using sulfur SAD data collected in vacuo using beamline I23 at Diamond Light Source. Complexes with l-arabinose, an iminosugar and two covalent inhibitors confirm the identity of
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bd17ba7265eb2563f1008e5fdeb969f2
http://hdl.handle.net/1887/3134980
http://hdl.handle.net/1887/3134980
Autor:
Isabel Bento, Michele Cianci, Guillaume Pompidor, Gleb Bourenkov, Thomas R. Schneider, Stefan Fiedler, Manfred Roessle, Johanna Kallio, Florent Cipriani, Ivars Karpics
Publikováno v:
Journal of Synchrotron Radiation
The P13 macromolecular crystallography beamline, based on the low-emittance source PETRA III, enables X-ray diffraction experiments on macromolecular crystals over a wide wavelength range (0.7–3.1 Å). The beam has a variable focus size and a small
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::18a683bcee530e7cdfbcdcec74d12983
http://europepmc.org/articles/PMC5182027
http://europepmc.org/articles/PMC5182027
Publikováno v:
Nuclear Instruments & Methods in Physics Research. Section B, Beam Interactions with Materials and Atoms
Phasing of novel macromolecular crystal structures has been challenging since the start of structural biology. Making use of anomalous diffraction of natively present elements, such as sulfur and phosphorus, for phasing has been possible for some sys
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::936944af6806e7eae8fd4980fb2cfa3a
http://europepmc.org/articles/PMC5727679
http://europepmc.org/articles/PMC5727679
Autor:
Nitish Sathyanarayanan, Jandhyam Srikanth, Nathan P. Coussens, Koichiro J. Yagi, Sanchari Banerjee, Leonard M. G. Chavas, Stephen S. Tobe, James S.S. Gray, Barbara Stay, S. Ramaswamy, François-Xavier Gallat
Publikováno v:
IUCrJ, Vol 3, Iss 4, Pp 282-293 (2016)
IUCrJ
IUCrJ
This article presents the features and structures of protein crystals naturally grown in vivo within developing embryos of the only known viviparous cockroach, D. punctata. This study reveals the heterogeneous nature of the crystalline protein with r
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::03fce8fcfca99ade8df5ac7bb55ed58b
http://scripts.iucr.org/cgi-bin/paper?S2052252516008903
http://scripts.iucr.org/cgi-bin/paper?S2052252516008903
Akademický článek
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Publikováno v:
IUCrJ, Vol 2, Iss 4, Pp 431-440 (2015)
IUCrJ
IUCrJ
Advancements in source stability, X-ray optics, detectors, goniometry, data-collection strategies and data reduction and phasing software made over the past decade have placed native SAD at the verge of becoming a ‘first-choice’ method for both d
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::26e1c852ba36bd0fa0d281a1fc38a71f
https://doi.org/10.1107/s2052252515008337
https://doi.org/10.1107/s2052252515008337