Zobrazeno 1 - 10
of 3 040
pro vyhledávání: '"specific primers"'
Autor:
Fuyong Li, Junhong Liu, María X. Maldonado-Gómez, Steven A. Frese, Michael G. Gänzle, Jens Walter
Publikováno v:
Microbiome, Vol 12, Iss 1, Pp 1-16 (2024)
Abstract Background Next-generation sequencing (NGS) approaches have revolutionized gut microbiome research and can provide strain-level resolution, but these techniques have limitations in that they are only semi-quantitative, suffer from high detec
Externí odkaz:
https://doaj.org/article/c9cefb5e53484958b2341378119b52cd
Autor:
Rachel Lissy Vargheese, S. Saravanan, S. Juliet Hepziba, S. Merina Prem Kumari, A. Kavitha Pushpam, Selvaraju Kanagarajan, M. Arumugam Pillai
Publikováno v:
BMC Plant Biology, Vol 24, Iss 1, Pp 1-11 (2024)
Abstract Background Improving the quality and shelf life of groundnut oil is one of the foremost objectives of groundnut breeding programmes. This can be achieved by marker-assisted introgression, a technique that efficiently and precisely enables br
Externí odkaz:
https://doaj.org/article/27f39c0191d8403699e4b3ca4c98d1e0
Publikováno v:
Zhongguo shuxue zazhi, Vol 37, Iss 6, Pp 660-665 (2024)
Objective To establish a method for qualitative detection of the presence or absence of all KIR genes by quantitative polymerase chain reaction(Q-PCR). Methods Based on the polymorphism of high-resolution level KIR alleles in Chinese population and t
Externí odkaz:
https://doaj.org/article/027fd7348db64a5bbef1035c325671d9
Publikováno v:
Shipin Kexue, Vol 45, Iss 9, Pp 102-108 (2024)
This study developed a real-time polymerase chain reaction (real-time PCR) method for the detection of Lactobacillus kefiranofaciens subsp. kefiranofaciens. Based on the 16S rDNA gene sequence and whole genome sequence of the type strain of L. kefira
Externí odkaz:
https://doaj.org/article/7ed37a3ecde24f3384db86ca787cefb6
Publikováno v:
Journal of Advanced Veterinary and Animal Research, Vol 11, Iss 2, Pp 247-258 (2024)
Objective: Meatballs are a popular meat-based food consumed widely in Indonesian society. However, the issue of unethical substitution of halal meatballs with non-halal meats, particularly pork and canine meat (CM), has emerged. The existence of non-
Externí odkaz:
https://doaj.org/article/4778c785c5ca4f1c9cf43619745857db
Publikováno v:
Journal of Nematology, Vol 56, Iss 1, Pp 34-48 (2024)
Hemp is a crop that has gained interest in Washington and Oregon. As with other crops, hemp production faces challenges due to biotic factors, including plant-parasitic nematodes. During a survey for plant-parasitic nematodes associated with hemp, Me
Externí odkaz:
https://doaj.org/article/b865e725a18f4aad95b26471609a5db1
Autor:
Huber Castillo-García
Publikováno v:
Revista Agrotecnológica Amazónica, Vol 4, Iss 2 (2024)
Coffee is one of the most relevant agricultural products globally, due to its significant economic, social and environmental impact, especially in producing countries such as Peru. In particular, the San Martín region stands out for its production o
Externí odkaz:
https://doaj.org/article/4d00d38eef414a6db202814146dd5cef
Autor:
Hans Ghezzi, Yiyun M. Fan, Katharine M. Ng, Juan C. Burckhardt, Deanna M. Pepin, Xuan Lin, Ryan M. Ziels, Carolina Tropini
Publikováno v:
mSphere, Vol 9, Iss 7 (2024)
ABSTRACT Characterizing microbial communities at high resolution and with absolute quantification is crucial to unravel the complexity and diversity of microbial ecosystems. This can be achieved with PCR assays, which enable highly selective detectio
Externí odkaz:
https://doaj.org/article/3725aec87b9143eaa49845519179e3ce
Autor:
Varun Arya, Srinivasa Narayana, Twinke Sinha, Aravindaram Kandan, Samantapudi Venkata Satyanarayana Raju
Publikováno v:
Frontiers in Plant Science, Vol 15 (2024)
The oriental fruit fly, Bactrocera dorsalis (Hendel), is a significant economic and quarantine pest due to its polyphagous nature. The accurate identification of B. dorsalis is challenging at the egg, maggot, and pupal stages, due to lack of distinct
Externí odkaz:
https://doaj.org/article/d1d9486b665e44e58874da479a68a669
Publikováno v:
Veterinary Sciences, Vol 11, Iss 10, p 466 (2024)
We developed and evaluated a semi-nested PCR assay for the detection of Babesia aktasi infection in goats based on the sequence of the B. aktasi 18S ribosomal RNA gene. Following in silico screening, the specificity of the primers was assessed using
Externí odkaz:
https://doaj.org/article/143ca12ca58c4258b58b7b13efbed2a7