OneFlowTraX: a user-friendly software for super-resolution analysis of single-molecule dynamics and nanoscale organization

Autor: Leander Rohr, Alexandra Ehinger, Luiselotte Rausch, Nina Glöckner Burmeister, Alfred J. Meixner, Julien Gronnier, Klaus Harter, Birgit Kemmerling, Sven zur Oven-Krockhaus

Publikováno v: Frontiers in Plant Science, Vol 15 (2024)

Super-resolution microscopy (SRM) approaches revolutionize cell biology by providing insights into the nanoscale organization and dynamics of macromolecular assemblies and single molecules in living cells. A major hurdle limiting SRM democratization

Externí odkaz: https://doaj.org/article/a06dd6226df14bdfa550d6aacdf4d169

Multi-phosphorylation reaction and clustering tune Pom1 gradient mid-cell levels according to cell size

Autor: Veneta Gerganova, Charlotte Floderer, Anna Archetti, Laetitia Michon, Lina Carlini, Thais Reichler, Suliana Manley, Sophie G Martin

Publikováno v: eLife, Vol 8 (2019)

Protein concentration gradients pattern developing organisms and single cells. In Schizosaccharomyces pombe rod-shaped cells, Pom1 kinase forms gradients with maxima at cell poles. Pom1 controls the timing of mitotic entry by inhibiting Cdr2, which f

Externí odkaz: https://doaj.org/article/78caafa2aa164861b81a82f8c11c1556

Single-molecule and super-resolution imaging of transcription in living bacteria

Autor: Stracy, M, Kapanidis, A

Publikováno v: Methods (San Diego, Calif.)

Highlights • Super-resolution imaging and tracking of RNA polymerase [RNAP] in living bacteria. • RNAP tracking reports on its intracellular mobility and spatial distribution. • RNAP spatial and diffusion profile is sensitive to antibiotics and

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid_dedup__::6636c0e2a77749d95297f7dd29ccf591
https://ora.ox.ac.uk/objects/uuid:68f81e77-eb6c-43b9-b2e0-af408c1a9582

Multi-phosphorylation reaction and clustering tune Pom1 gradient mid-cell levels according to cell size.

Autor: Gerganova V; Department of Fundamental Microbiology, Faculty of Biology and Medicine, University of Lausanne, Lausanne, Switzerland., Floderer C; Institute of Physics, School of Basic Science, École Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland., Archetti A; Institute of Physics, School of Basic Science, École Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland., Michon L; Department of Fundamental Microbiology, Faculty of Biology and Medicine, University of Lausanne, Lausanne, Switzerland., Carlini L; Institute of Physics, School of Basic Science, École Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland., Reichler T; Department of Fundamental Microbiology, Faculty of Biology and Medicine, University of Lausanne, Lausanne, Switzerland., Manley S; Institute of Physics, School of Basic Science, École Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland., Martin SG; Department of Fundamental Microbiology, Faculty of Biology and Medicine, University of Lausanne, Lausanne, Switzerland.

Publikováno v: ELife [Elife] 2019 May 03; Vol. 8. Date of Electronic Publication: 2019 May 03.