A mixing heteroduplex mobility assay (mHMA) to genotype homozygous mutants with small indels generated by CRISPR-Cas9 nucleases

Autor: Samantha D. Foster, Ashley N. Turner, Sarah R. Glover, Kiranam Chatti, Anil K. Challa

Publikováno v: MethodsX

Graphical abstract Abstract The development of gene editing technologies, especially the CRISPR-Cas9 system, has been pivotal for understanding the functional role of proteins. Rapid and efficient genotyping methods are necessary to screen for genera

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https://doi.org/10.1016/j.mex.2018.11.017

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A mixing heteroduplex mobility assay (mHMA) to genotype homozygous mutants with small indels generated by CRISPR-Cas9 nucleases.

Autor: Foster SD; Science and Technology Honors Program, University of Alabama at Birmingham (UAB), United States of America.; Undergraduate Neuroscience Program, UAB, United States of America., Glover SR; Science and Technology Honors Program, University of Alabama at Birmingham (UAB), United States of America.; Department of Biology, UAB, United States of America., Turner AN; Department of Biology, UAB, United States of America., Chatti K; Dr. Reddy's Institute of Life Sciences, Hyderabad, India., Challa AK; Department of Genetics, UAB, United States of America.

Publikováno v: MethodsX [MethodsX] 2018 Nov 27; Vol. 6, pp. 1-5. Date of Electronic Publication: 2018 Nov 27 (Print Publication: 2019).