Identifiering av promotorregionen för Cyt c Id1 samt undersökning av genuttrycket i närvarooch frånvaro av syre

Autor: Nabo, Slava

In this work, the identification of the promoter for c-cytochromes Cyt c Id-1 in Ideonella dechloratans has been investigated. Additionally, the study examined investigating whether gene expression is affected by the presence and absence of oxygen. T

Externí odkaz: http://urn.kb.se/resolve?urn=urn:nbn:se:kau:diva-97108

Comparison of In-Frame Deletion, Homology-Directed Repair, and Prime Editing-Based Correction of Duchenne Muscular Dystrophy Mutations

Autor: Xiaoying Zhao, Kunli Qu, Benedetta Curci, Huanming Yang, Lars Bolund, Lin Lin, Yonglun Luo

Publikováno v: Zhao, X, Qu, K, Curci, B, Yang, H, Bolund, L, Lin, L & Luo, Y 2023, ' Comparison of In-Frame Deletion, Homology-Directed Repair, and Prime Editing-Based Correction of Duchenne Muscular Dystrophy Mutations ', Biomolecules, vol. 13, no. 5 . https://doi.org/10.3390/biom13050870
Zhao, X, Qu, K, Curci, B, Yang, H, Bolund, L, Lin, L & Luo, Y 2023, ' Comparison of In-Frame Deletion, Homology-Directed Repair, and Prime Editing-Based Correction of Duchenne Muscular Dystrophy Mutations ', Biomolecules, vol. 13, no. 5, 870 . https://doi.org/10.3390/biom13050870

Recent progress in CRISPR gene editing tools has substantially increased the opportunities for curing devastating genetic diseases. Here we compare in-frame deletion by CRISPR-based non-homologous blunt end joining (NHBEJ), homology-directed repair (

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ac6cdeb48dba52b40a1385288c112b23
https://doi.org/10.3390/biom13050870

Tento výsledek nelze pro nepřihlášené uživatele zobrazit.
K zobrazení výsledku je třeba se přihlásit.

Influence of the reporter vector backbone on 2-deoxyglucose dependent promoter activation

Autor: Mateusz Wawro, Aleksandra Wielento, Joanna Jurczak, Aleksandra Solecka, Weronika Sowinska, Aneta Kasza

Publikováno v: Acta biochimica Polonica. 67(3)

Reporter vectors are very often used to investigate mechanisms responsible for regulation of promoter activity. Since their first generation, many new variants were constructed to increase sensitivity and reduce background signal. However, these tool

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::52d7f18e9e9e2dfea93bf4f319c0ef66
https://pubmed.ncbi.nlm.nih.gov/32886468

Functional Evaluation of CRISPR Activity by the Dual-Fluorescent Surrogate System: C-Check

Autor: Yonglun Luo, Lin Lin

Publikováno v: Methods in Molecular Biology ISBN: 9781493991693
Lin, L & Luo, Y 2019, Functional Evaluation of CRISPR Activity by the Dual-Fluorescent Surrogate System : C-Check . in Y Luo (ed.), CRISPR Gene Editing : Methods and Protocols . Springer Science+Business Media, Methods in Molecular Biology, vol. 1961, pp. 67-77 . https://doi.org/10.1007/978-1-4939-9170-9_5

Rapid evaluation of the CRISPR gRNA activity is an essential step of employing the technology in editing genes. Through machine learning strategy, the rule sets for in silico designing gRNAs with high activity has greatly improved. However, there are

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5d1a94bd8c20b6c74425d4df4c723be2
https://doi.org/10.1007/978-1-4939-9170-9_5