Zobrazeno 1 - 10
of 5 894
pro vyhledávání: '"reference genes"'
Autor:
Ziwei Chen, Guoying Hua, Xin Shu, Wuchao Zhuang, Jilong Zhang, Runbang Zhu, Xiaotong Zheng, Jianfei Chen
Publikováno v:
Scientific Reports, Vol 14, Iss 1, Pp 1-13 (2024)
Abstract The liver plays a vital role in lipid synthesis and metabolism in poultry. To study the functional genes more effectively, it is essential to screen of reliable reference genes in the chicken liver, including females, males, embryos, as well
Externí odkaz:
https://doaj.org/article/a88641c026224610aca04bcf46bc69fa
Autor:
Márcio Wilson Dias de Brito, Stephanie Serafim de Carvalho, Maria Beatriz dos Santos Mota, Rafael Dias Mesquita
Publikováno v:
BMC Genomics, Vol 25, Iss 1, Pp 1-12 (2024)
Abstract Background Real-time quantitative PCR (RT-qPCR) is one of the most widely used gene expression analyses for validating RNA-seq data. This technique requires reference genes that are stable and highly expressed, at least across the different
Externí odkaz:
https://doaj.org/article/be62f33df9cc440fa6d6959fc1c9c170
Publikováno v:
Diabetes, Metabolic Syndrome and Obesity, Vol Volume 17, Pp 1081-1091 (2024)
Yuanli Ai,1,* Kun Peng,2,* Chunli Li,1,* Jun Zhang,1 Gang Wang,3,4 Bin Wang,1 Enyi Huang3,4 1Institute of Life Sciences, College of Basic Medicine, Chongqing Medical University, Chongqing, People’s Republic of China; 2Chongqing Medical
Externí odkaz:
https://doaj.org/article/34d78aa7686249cea2caf17e8c0f08ae
Publikováno v:
Fruit Research, Vol 4, Iss 1, Pp 1-7 (2024)
The rapid, reliable, and efficient characteristics of quantitative reverse transcription polymerase chain reaction (qRT-PCR) make it a highly advantageous method for assessing gene expression levels. The identification of stable reference genes is cr
Externí odkaz:
https://doaj.org/article/73ac0baa39384a7ba3eef19547a1e175
Autor:
Haiyang Chi, Shasha Bai, Chenxiao Chen, Yuying Bao, Xiaoxin Qu, Shitao Sun, Jiangpeng Pan, Xiushi Yang, Chunsheng Hou, Yanchun Deng
Publikováno v:
Plant Stress, Vol 14, Iss , Pp 100575- (2024)
Squalene plays a crucial role in plant growth, development, and stress tolerance. To select the best reference genes (RGs) for expression profile analysis of genes involved in squalene biosynthesis in olives under stress tolerance, the expression sta
Externí odkaz:
https://doaj.org/article/c36af6ab1c594646a52abdf7bb7e0139
Autor:
Dmitrii E. Khramov, Olga I. Nedelyaeva, Alena O. Konoshenkova, Vadim S. Volkov, Yurii V. Balnokin
Publikováno v:
Communicative & Integrative Biology, Vol 17, Iss 1 (2024)
Сoding sequences of seven housekeeping genes: actin SaACT7, ubiquitin-conjugating protein SaUBC10, glyceraldehyde-3-phosphate dehydrogenase SaGAPDH, protein of the large subunit of ribosomes SaL2, α-tubulin SaTUA, translation elongation factor SaeE
Externí odkaz:
https://doaj.org/article/fd9ca9302e7942918d2ed2ca42e7e810
Autor:
Yiqun Li, Jingjing Zhang, Mingyang Xue, Yong Zhou, Nan Jiang, Yan Meng, Chen Xu, Jinyu Shen, Yuding Fan
Publikováno v:
Comparative Immunology Reports, Vol 6, Iss , Pp 200134- (2024)
Quantitative Real-time PCR (qRT-PCR) is one of the most widely used methods for gene quantification because it is extremely sensitive, can be highly sequence specific, and has high precision while being easily reproducible in samples. The stability o
Externí odkaz:
https://doaj.org/article/03020efc9c8e47b19bcf4f6892ca212f
Publikováno v:
Data in Brief, Vol 53, Iss , Pp 110221- (2024)
Real-time quantitative PCR (RT-qPCR) is a widely used method for accurate quantitative gene expression analysis. For accurate quantitative verification of RT-qPCR, it is essential to select a reference gene with high expression stability depending on
Externí odkaz:
https://doaj.org/article/e753cdcb095940fbaf5840374987fdcd
Autor:
Jinxin Miao, Le Kang, Tianfeng Lan, Jianyao Wang, Siqing Wu, Yifan Jia, Xia Xue, Haoran Guo, Pengju Wang, Yan Li
Publikováno v:
Animal Models and Experimental Medicine, Vol 6, Iss 6, Pp 609-618 (2023)
Abstract Background Acute pancreatitis (AP) is a severe disorder that leads to high morbidity and mortality. Appropriate reference genes are important for gene analysis in AP. This study sought to study the expression stability of several reference g
Externí odkaz:
https://doaj.org/article/a5b75534dd444fbc821c4f13b7a0f84c
Autor:
Yaping Liu, Wanting Zhong, Shuo Feng, Ziteng Tang, Yingzhi Zhang, Lianzhong Ai, Zhiqiang Xiong
Publikováno v:
Journal of Dairy Science, Vol 106, Iss 11, Pp 7477-7485 (2023)
ABSTRACT: Bifidobacterium animalis, one of the predominant bacteria in the intestines of humans and other mammals, is widely added to dairy products. We employed RNA sequencing to analyze gene expression variance on a genome-wide scale and found stab
Externí odkaz:
https://doaj.org/article/dcb5e209876649359a21f2cd7651d221