Zobrazeno 1 - 10
of 1 238
pro vyhledávání: '"raav"'
Publikováno v:
Virology Journal, Vol 21, Iss 1, Pp 1-10 (2024)
Abstract Background In recent years, gene therapy drugs have been widely marketed, and their effectiveness and potential have been confirmed. Thus, increasing their production on an industrial scale is critical. Recombinant adeno-associated viruses (
Externí odkaz:
https://doaj.org/article/df07bcbad12f42859b6236a861c13e71
Publikováno v:
Analytica, Vol 5, Iss 2, Pp 263-272 (2024)
Rapid advancements in gene technology have raised concerns regarding the potential abuse of techniques, such as gene doping, for enhancing athletic performance. To identify this possibility, a reliable procedure for detecting doping genes is required
Externí odkaz:
https://doaj.org/article/929a629f2e244db09078d6f7746b9b7d
Autor:
Samantha Schrecke, Kevin McManus, Cassandra Moshfegh, Jessica Stone, Thuy-Uyen Nguyen, Gustavo Rivas, Ismaeel Muhamed, Daniel A. J. Mitchell
Publikováno v:
Frontiers in Bioengineering and Biotechnology, Vol 12 (2024)
Cell and gene therapy (CGT) is a field of therapeutic medicine that aims to treat, prevent, and cure diseases using engineered cells (stem cells, immune cells, and differentiated adult or fetal cells), vectors [Adeno Associated Virus (AAV), Adeno Vir
Externí odkaz:
https://doaj.org/article/9d1bb476a2d1478a914c1f3c78361416
Publikováno v:
Molecular Therapy: Methods & Clinical Development, Vol 32, Iss 3, Pp 101304- (2024)
The viral genome titer is a crucial indicator for the clinical dosing, manufacturing, and analytical testing of recombinant adeno-associated virus (rAAV) gene therapy products. Although quantitative PCR and digital PCR are the common methods used for
Externí odkaz:
https://doaj.org/article/f9b25be58665436e9e8eed3cd2048d17
Autor:
Xueyuan Liu, Riffard Jean-Gilles, Julia Baginski, Christina Cai, Ruilan Yan, Lili Zhang, Kevin Lance, Johannes C.M. van der Loo, Beverly L. Davidson
Publikováno v:
Molecular Therapy: Methods & Clinical Development, Vol 32, Iss 3, Pp 101298- (2024)
Recombinant adeno-associated virus (rAAV)-based gene therapies are expanding in their application. Despite progress in manufacturing, current analytical methods for product quantification and characterization remain largely unchanged. Although critic
Externí odkaz:
https://doaj.org/article/2f77cd4b3e5c4b8fb79a86dae4ba5380
Autor:
Eduard H.T.M. Ebberink, Alisa Ruisinger, Markus Nuebel, Helena Meyer-Berg, Irene R.S. Ferreira, Marco Thomann, Albert J.R. Heck
Publikováno v:
Molecular Therapy: Methods & Clinical Development, Vol 32, Iss 3, Pp 101293- (2024)
Adeno-associated viruses (AAVs) are gaining traction as delivery vehicles for gene therapy although the molecular understanding of AAV-transgene release is still limited. Typically, the process of viral uncoating is investigated (in vitro) through th
Externí odkaz:
https://doaj.org/article/b6751d5aab234cb4ab56c73d1e7c9680
Publikováno v:
Molecular Therapy: Methods & Clinical Development, Vol 32, Iss 3, Pp 101278- (2024)
The gene therapy field seeks cost-effective, large-scale production of recombinant adeno-associated virus (rAAV) vectors for high-dosage therapeutic applications. Although strategies like suspension cell culture and transfection optimization have sho
Externí odkaz:
https://doaj.org/article/5a654ce809564105a4daed88586168a5
Autor:
Hao Liu, Yue Zhang, Mitchell Yip, Lingzhi Ren, Jialing Liang, Xiupeng Chen, Nan Liu, Ailing Du, Jiaming Wang, Hao Chang, Hyejin Oh, Chen Zhou, Ruxiao Xing, Mengyao Xu, Peiyi Guo, Dominic Gessler, Jun Xie, Phillip W.L. Tai, Guangping Gao, Dan Wang
Publikováno v:
Molecular Therapy: Methods & Clinical Development, Vol 32, Iss 2, Pp 101230- (2024)
Recombinant adeno-associated virus (rAAV)-based gene therapy is entering clinical and commercial stages at an unprecedented pace. Triple transfection of HEK293 cells is currently the most widely used platform for rAAV manufacturing. Here, we develop
Externí odkaz:
https://doaj.org/article/60f1fe4d77694132aa0fbf4e90bcd7e3
Publikováno v:
Pharmaceuticals, Vol 17, Iss 9, p 1213 (2024)
Recombinant AAV (rAAV) vectors are increasingly favored for gene therapy due to their useful features of vectorology, such as transfection of dividing and nondividing cells, the presence of tissue-specific serotypes, and biosafety considerations. Thi
Externí odkaz:
https://doaj.org/article/3fd507c12c81476d9f2a022a96294cef
Publikováno v:
Jichu yixue yu linchuang, Vol 43, Iss 10, Pp 1491-1497 (2023)
Objective To provide a basis for gene therapy of sporadic Parkinson's disease (PD) caused by mitochondrial complex Ⅰ dysfunction, yeast complex Ⅰ (expressed by internal NADH dehydrogenase,NDI1) was tested to replace human complex Ⅰ with functio
Externí odkaz:
https://doaj.org/article/251fca3670f148e7ac72b884a8bf489f