Zobrazeno 1 - 7
of 7
pro vyhledávání: '"quantitative PCR instrument"'
Publikováno v:
Applied Sciences, Vol 14, Iss 12, p 5031 (2024)
The fluorescence quantitative polymerase chain reaction (qPCR) instrument has been widely used in molecular biology applications, where the reliability of the qPCR performance directly affects the accuracy of its detection results. In this paper, an
Externí odkaz:
https://doaj.org/article/0356d375cf484a70832374be8f2cf866
Akademický článek
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Publikováno v:
Analytica Chimica Acta. 1109:140-147
Active DNA demethylation process critically relies on the intrinsic properties of ten-eleven translocation proteins (Tets), particularly the flanking sequence preference. Challenged by the fact that the proximate bases to the 5-methylcytosine (5mC) a
Publikováno v:
Industrial & Engineering Chemistry Research. 58:9665-9674
We report improvements on the heat transfer and on the control strategy of a thermal cycler implemented on a novel device for performing a fast polymerase chain reaction (PCR). The reduction of the thermal mass of the sample holder and the direct con
Autor:
Zhai Chao, Aitao Li, Shuliang Chen, Lixin Ma, Longyu Wang, Jun Yang, Ruyi He, Yang Liu, Linlin Liu, Xiao Yu, Fei Wang
Publikováno v:
Biosensors and Bioelectronics
Biosensors & Bioelectronics
Biosensors & Bioelectronics
In the present study, we upgraded Pyrococcus furiosus Argonaute (PfAgo) mediated nucleic acid detection method and established a highly sensitive and accurate molecular diagnosis platform for the large-scale screening of COVID-19 infection. Briefly,
Autor:
Yanfei Zhang, Yuzhi Xu, Yingjun Ma, Yang Wang, Junjie Zhang, Zong Dai, Xiaoyong Zou, Danping Chen, Si-Yang Liu, Li Zhang, Mingming Mo, Jun Chen, Chun Cai
Publikováno v:
Nucleic Acids Research
The current methods for quantifying genome-wide 5-methylcytosine (5mC) oxides are still scarce, mostly restricted with two limitations: assay sensitivity is seriously compromised with cost, assay time and sample input; epigenetic information is irrep
Publikováno v:
BMEI
Quantitative Polymerase Chain Reaction (qPCR) has revolutionized the detection of DNA and RNA. But the quantification results are susceptible to the effects of background noise and the intensity of detected fluorescent signals. Aiming at solving the