Výsledky vyhledávání - "qPCR, quantitative PCR"

Akademický článek

Nitrification inhibitor chlorate and nitrogen substrates differentially affect comammox Nitrospira in a grassland soil

Publikováno v: Frontiers in Microbiology, Vol 15 (2024)

IntroductionThrough the combined use of two nitrification inhibitors, Dicyandiamide (DCD) and chlorate with nitrogen amendment, this study aimed to investigate the contribution of comammox Nitrospira clade B, ammonia oxidizing bacteria (AOB) and arch

Externí odkaz: https://doaj.org/article/fd09d98cc2794f2ab642ce3797d2e80d

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Akademický článek

Unveiling APOL1 haplotypes in a predominantly African-American cohort of kidney transplant patients: a novel classification using probe-independent quantitative real-time PCR

Autor: Murat Dogan, Christine Watkins, Holly Ingram, Nicholas Moore, Grace M. Rucker, Elizabeth G. Gower, James D. Eason, Anshul Bhalla, Manish Talwar, Nosratollah Nezakatgoo, Corey Eymard, Ryan Helmick, Jason Vanatta, Amandeep Bajwa, Canan Kuscu, Cem Kuscu

Publikováno v: Frontiers in Medicine, Vol 11 (2024)

IntroductionApolipoprotein-L1 (APOL1) is a primate-specific protein component of high-density lipoprotein (HDL). Two variants of APOL1 (G1 and G2), provide resistance to parasitic infections in African Americans but are also implicated in kidney-rela

Externí odkaz: https://doaj.org/article/90aa22aae60f4501bd8dd9857dc88b19

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Akademický článek

Molecular diagnosis of opportunistic infections in the central nervous system of HIV-infected adults in Manaus, Amazonas

Autor: Sabrina Araújo de Melo, Sérgio Damasceno Pinto, Ewerton da Silva Ferreira, Reinan Brotas, Eveny Perlize Melo Marinho, Valderjane Aprigio da Silva, Rossiclea Lins Monte, Pablo Vinícius Silveira Feitoza, Monique Freire Reis, Taynná V. Rocha Almeida, Luiz Carlos de Lima Ferreira, Michele de Souza Bastos

Publikováno v: Frontiers in Medicine, Vol 10 (2024)

BackgroundOpportunistic infections in the central nervous system (CNS) of people with HIV/AIDS (PLWHA) remain significant contributors to morbidity and mortality, especially in resource-limited scenarios. Diagnosing these infections can be challengin

Externí odkaz: https://doaj.org/article/51e2417e38e747fca48326274f565b0b

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Akademický článek

New methods for the quantification of mixed chimerism in transplantation

Autor: Christophe Picard, Coralie Frassati, Nicem Cherouat, Sandrine Maioli, Philippe Moskovtchenko, Mathilde Cherel, Jacques Chiaroni, Pascal Pedini

Publikováno v: Frontiers in Immunology, Vol 14 (2023)

BackgroundQuantification of chimerism showing the proportion of the donor in a recipient is essential for the follow-up of hematopoietic stem cell transplantation but can also be useful to document an immune tolerance situation after solid organ tran

Externí odkaz: https://doaj.org/article/ad37111c4e01415a98f6110008043ded

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Akademický článek

Detection and quantification of bacterial species DNA in bovine digital dermatitis lesions in swabs and fine-needle aspiration versus biopsies

Autor: Angelica Petersen Dias, Jeroen De Buck

Publikováno v: Frontiers in Veterinary Science, Vol 9 (2022)

Digital Dermatitis (DD) is a polymicrobial disease characterized by ulcerative lesions on the heel bulb of cattle and for which, despite being reported almost 50 years ago, information on the causative agent is still lacking. Tissue biopsies are regu

Externí odkaz: https://doaj.org/article/8070dd1b5a0945ce8b53646060c34023

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Akademický článek

It Takes Two to Tango: Combining Conventional Culture With Molecular Diagnostics Enhances Accuracy of Streptococcus pneumoniae Detection and Pneumococcal Serogroup/Serotype Determination in Carriage

Autor: Willem R. Miellet, Janieke van Veldhuizen, David Litt, Rob Mariman, Alienke J. Wijmenga-Monsuur, Paul Badoux, Tessa Nieuwenhuijsen, Rebecca Thombre, Sanaa Mayet, Seyi Eletu, Carmen Sheppard, Marianne Alice van Houten, Nynke Y. Rots, Elizabeth Miller, Norman K. Fry, Elisabeth A. M. Sanders, Krzysztof Trzciński

Publikováno v: Frontiers in Microbiology, Vol 13 (2022)

BackgroundThe specificity of molecular methods for the detection of Streptococcus pneumoniae carriage is under debate. We propose a procedure for carriage surveillance and vaccine impact studies that increases the accuracy of molecular detection of l

Externí odkaz: https://doaj.org/article/87b6911c6e95436e94f1182f4ddfd734

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Akademický článek

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Akademický článek

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Polyoxypregnanes as safe, potent, and specific ABCB1-inhibitory pro-drugs to overcome multidrug resistance in cancer chemotherapy in vitro and in vivo

Autor: Kenneth K.W. To, Jiang Ma, Stella Chai, Chunyuan Zhang, Chun Yin, Sheng Yao, Xu Wu, Thomas Efferth, Yang Ye, Onat Kadioglu, Ge Lin

Publikováno v: Acta Pharmaceutica Sinica. B
Acta Pharmaceutica Sinica B, Vol 11, Iss 7, Pp 1885-1902 (2021)

Multidrug resistance (MDR) mediated by ATP binding cassette subfamily B member 1 (ABCB1) is significantly hindering effective cancer chemotherapy. However, currently, no ABCB1-inhibitory drugs have been approved to treat MDR cancer clinically, mainly

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b10ca45cdbb87dcf2ef29e5865d5f864
https://doi.org/10.1016/j.apsb.2020.12.021

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Whole exome sequencing, in silico and functional studies confirm the association of the GJB2 mutation p.Cys169Tyr with deafness and suggest a role for the TMEM59 gene in the hearing process

Autor: Mona Mahfood, Rania Harati, Jihen Chouchen, Abdullah Al Mutery, Walaa Kamal Eddine Ahmad Mohamed, Abdelaziz Tlili

Publikováno v: Saudi Journal of Biological Sciences
Saudi Journal of Biological Sciences, Vol 28, Iss 8, Pp 4421-4429 (2021)

The development of next generation sequencing techniques has facilitated the detection of mutations at an unprecedented rate. These efficient tools have been particularly beneficial for extremely heterogeneous disorders such as autosomal recessive no

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f64784a76361bd03c51d0cb19e0388e9
http://europepmc.org/articles/PMC8324942

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