Zobrazeno 1 - 10
of 214
pro vyhledávání: '"pyrG"'
Publikováno v:
Current Issues in Molecular Biology, Vol 45, Iss 6, Pp 4778-4795 (2023)
Nanobodies, also referred to as VHH antibodies, are the smallest fragments of naturally produced camelid antibodies and are ideal affinity reagents due to their remarkable properties. They are considered an alternative to monoclonal antibodies (mAbs)
Externí odkaz:
https://doaj.org/article/267918c129804daa8f8120152de887d2
Publikováno v:
Shipin gongye ke-ji, Vol 44, Iss 1, Pp 200-207 (2023)
Objective: An auxotrophic strain with high efficiency of homologous recombination was developed in Aspergillus oryzae RIB40. Methods: The AopyrG mutant of A. oryzae RIB40 was firstly obtained using a homologous recombination technique and the 5-fluor
Externí odkaz:
https://doaj.org/article/d70079041c1a4f3a82dee9bcbef0d3eb
Akademický článek
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Akademický článek
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Autor:
Dede Abdulrachman, Lily Eurwilaichitr, Verawat Champreda, Duriya Chantasingh, Kusol Pootanakit
Publikováno v:
BMC Biotechnology, Vol 21, Iss 1, Pp 1-13 (2021)
Abstract Background CRISPR-Cas genome editing technologies have revolutionized biotechnological research particularly in functional genomics and synthetic biology. As an alternative to the most studied and well-developed CRISPR/Cas9, a new class 2 (t
Externí odkaz:
https://doaj.org/article/067c59e17545427f81583a61d047bb46
Autor:
Tatpong Boontawon, Takehito Nakazawa, Chikako Inoue, Keishi Osakabe, Moriyuki Kawauchi, Masahiro Sakamoto, Yoichi Honda
Publikováno v:
AMB Express, Vol 11, Iss 1, Pp 1-11 (2021)
Abstract Pleurotus ostreatus is one of the most commercially produced edible mushrooms worldwide. Improved cultivated strains with more useful traits have been obtained using classical breeding, which is laborious and time-consuming. Here, we attempt
Externí odkaz:
https://doaj.org/article/7acd846d12264b8ba513af41966a984e
Autor:
Lihui Zhang, Xiaomei Zheng, Timothy C. Cairns, Zhidan Zhang, Depei Wang, Ping Zheng, Jibin Sun
Publikováno v:
Microbial Cell Factories, Vol 19, Iss 1, Pp 1-12 (2020)
Abstract Background Aspergillus niger is a filamentous fungus used for the majority of global citric acid production. Recent developments in genome editing now enable biotechnologists to engineer and optimize A. niger. Currently, however, genetic-lea
Externí odkaz:
https://doaj.org/article/3aee4e310081469d86fec26ecf45161c
Autor:
Satoru Shinkawa, Shigenobu Mitsuzawa
Publikováno v:
Biotechnology for Biofuels, Vol 13, Iss 1, Pp 1-15 (2020)
Abstract Background The development of biorefinery systems that use lignocellulosic biomass as a renewable carbon source to produce fuels and chemicals is attracting increasing attention. The process cost of enzymatic saccharification of biomass is a
Externí odkaz:
https://doaj.org/article/fb83be346fc24e7cbc0f807b6ee9a49f
Publikováno v:
Journal of Fungi, Vol 9, Iss 3, p 302 (2023)
Marine-derived fungi are emerging as prolific workhorses of structurally novel natural products (NPs) with diverse bioactivities. However, the limitation of available selection markers hampers the exploration of cryptic NPs. Recyclable markers are th
Externí odkaz:
https://doaj.org/article/854ca5f9fb8140b892d1722c9884057b
A Simple and Efficient CRISPR/Cas9 System Using a Ribonucleoprotein Method for Flammulina filiformis
Autor:
Jianyu Liu, Haiyang Cui, Ruijuan Wang, Zhen Xu, Hailong Yu, Chunyan Song, Huan Lu, Qiaozhen Li, Danrun Xing, Qi Tan, Weiming Sun, Gen Zou, Xiaodong Shang
Publikováno v:
Journal of Fungi, Vol 8, Iss 10, p 1000 (2022)
CRISPR/Cas9 systems were established in some edible fungi based on in vivo expressed Cas9 and guide RNA. Compared with those systems, the in vitro assembled Cas9 and sgRNA ribonucleoprotein complexes (RNPs) have more advantages, but only a few exampl
Externí odkaz:
https://doaj.org/article/3a208ded07544539b3d16ca4e2776d97