Zobrazeno 1 - 10
of 851
pro vyhledávání: '"protein expression and purification"'
Publikováno v:
STAR Protocols, Vol 5, Iss 3, Pp 103294- (2024)
Summary: The tumor suppressor breast cancer 1 (BRCA1) complexed with BRCA1-associated RING domain 1 (BARD1), a RING-type E3 ligase, facilitates the attachment of ubiquitin onto the substrate protein. Here, we present a protocol for evaluating the E3
Externí odkaz:
https://doaj.org/article/6e718f931beb4697a100cdcab231bcc1
Autor:
Li Huang, Zheng Zhou
Publikováno v:
STAR Protocols, Vol 5, Iss 3, Pp 103295- (2024)
Summary: The histone lysine methyltransferase SUV420H1 preferentially targets the H2A.Z-containing nucleosome core particle (H2A.Z-NCP) and catalyzes the H4K20me2 modification at replication origins. Here, we present a protocol for preparing SUV420H1
Externí odkaz:
https://doaj.org/article/c0419efcd6d6408eb62d9920c08ec4ab
Publikováno v:
STAR Protocols, Vol 5, Iss 3, Pp 103296- (2024)
Summary: Here, we present an optimized acyl-PEGyl exchange gel shift (APEGS) assay to monitor palmitoylation of high-molecular-weight proteins from primary neuronal cultures. We describe steps for culturing cortical neurons from rat embryos and expre
Externí odkaz:
https://doaj.org/article/aa9058d975c34e9b9f4266b245131bb4
Autor:
Qiwei Huang, CongBao Kang
Publikováno v:
STAR Protocols, Vol 5, Iss 3, Pp 103278- (2024)
Summary: Fragment-based drug design plays an important role in drug discovery. Protein-observed NMR experiments with isotopically labeled samples are used to probe target-ligand interactions and map the ligand-binding sites. Here, we present a protoc
Externí odkaz:
https://doaj.org/article/76ca847df3c245c1aebf0e9abc63b0cc
Publikováno v:
STAR Protocols, Vol 5, Iss 3, Pp 103275- (2024)
Summary: Heat shock (HS) coincides with the assembly of translationally arrested heat shock messenger ribonucleoprotein particles (HS-mRNPs) and condensates. Here, we present a protocol to reconstitute HS-mRNPs and HS condensates with eIF4G, eIF4E, P
Externí odkaz:
https://doaj.org/article/b111396c447f4359803cc261fe35a690
Publikováno v:
STAR Protocols, Vol 5, Iss 3, Pp 103237- (2024)
Summary: Here, we present a large-scale FLAG immunoprecipitation protocol to isolate large protein complexes driving DNA replication at replicating chromatin assembled in Xenopus laevis egg extract. We describe how to prepare demembranated sperm nucl
Externí odkaz:
https://doaj.org/article/d3a3b49bd9604fb0a3137a6c26adea9e
Publikováno v:
STAR Protocols, Vol 5, Iss 3, Pp 103182- (2024)
Summary: S-acylation of proteins allows their association with membranes. Here, we present a protocol for establishing a platform for membrane affinity evaluation of S-acylated proteins in vitro. We describe steps for preparing lipid-maleimide compou
Externí odkaz:
https://doaj.org/article/1b83aab1a76049d198ce83569b399657
Publikováno v:
STAR Protocols, Vol 5, Iss 3, Pp 103171- (2024)
Summary: Here, we present a protocol to quantify interactions among difficult-to-express proteins from Drosophila cells using the select western blot-free tagged-protein interaction (SWFTI) assay. We describe steps for plasmid design, cell plating, p
Externí odkaz:
https://doaj.org/article/63f979aa131f4ea3a13b457ed2186e02
Publikováno v:
STAR Protocols, Vol 5, Iss 4, Pp 103454- (2024)
Summary: Activating transcription factor 4 (ATF4) is a key player in the integrated stress response, whose expression is subject to tight translational control. Studying its stress-provoked induction, accompanied by the general translational shutdown
Externí odkaz:
https://doaj.org/article/ec47571144654d728002c7bfb6b548c9
Publikováno v:
STAR Protocols, Vol 5, Iss 4, Pp 103366- (2024)
Summary: The ribosome-associated protein quality control (RQC) core factor nuclear export mediator factor (NEMF) appends C-terminal extended sequences (CESs) to ribosome-stalled nascent chains (NCs). Specific CESs compositions could be directly recog
Externí odkaz:
https://doaj.org/article/8c432919785047d5ac98c9d3cc504d1e