Zobrazeno 1 - 10
of 820
pro vyhledávání: '"prime editing"'
Autor:
Zhiquan Liu, Siyu Chen, Alexander E. Davis, Chien‐Hui Lo, Qing Wang, Tingting Li, Ke Ning, Qi Zhang, Jingyu Zhao, Sui Wang, Yang Sun
Publikováno v:
Advanced Science, Vol 11, Iss 42, Pp n/a-n/a (2024)
Abstract Retinitis pigmentosa (RP) is a complex spectrum of inherited retinal diseases marked by the gradual loss of photoreceptor cells, ultimately leading to blindness. Among these, mutations in PDE6A, responsible for encoding a cGMP‐specific pho
Externí odkaz:
https://doaj.org/article/0b14da0027c5482cb48780d61d2b3cb4
Autor:
Luis Guilherme Virgilio Fernandes, Camila Hamond, Bienvenido W. Tibbs-Cortes, Ellie J. Putz, Steven C. Olsen, Mitchell V. Palmer, Jarlath E. Nally
Publikováno v:
mBio, Vol 15, Iss 9 (2024)
ABSTRACT Leptospirosis, caused by pathogenic bacteria from the genus Leptospira, is a global zoonosis responsible for more than one million human cases and 60,000 deaths annually. The disease also affects many domestic animal species. Historically, g
Externí odkaz:
https://doaj.org/article/6d6ad5fd0c1a42a388173c15ceff3104
Autor:
Xiaoshuang Liu, Dongfang Gu, Yiru Zhang, Yingli Jiang, Zhi Xiao, Rongfang Xu, Ruiying Qin, Juan Li, Pengcheng Wei
Publikováno v:
Genome Biology, Vol 25, Iss 1, Pp 1-18 (2024)
Abstract Background High-efficiency prime editing (PE) is desirable for precise genome manipulation. The activity of mammalian PE systems can be largely improved by inhibiting DNA mismatch repair by coexpressing a dominant-negative variant of MLH1. H
Externí odkaz:
https://doaj.org/article/6b5a316d38b349c8a7375e4dd156cca6
Autor:
Congwen Shao, Qing Liu, Jinchao Xu, Jianxiang Zhang, Chengpeng Zhang, Ye Xin, Yuhua Ye, Bin Lin, Xinhua Zhang, Li Cheng, Xiangmin Xu, Peng Xu
Publikováno v:
Molecular Therapy: Nucleic Acids, Vol 35, Iss 4, Pp 102371- (2024)
Hemoglobin Constant Spring (Hb CS) is the most common non-deletional and clinically significant α-thalassemic mutation, and it is caused by an anti-termination mutation at the α2-globin gene stop codon. We developed a prime editing strategy for the
Externí odkaz:
https://doaj.org/article/1865a64661dc4bcab7577484b5cdddd4
Autor:
Amjad Hussain, Mamoona Munir, Awais Khalid, Musrat Ali, Mohammed Amanullah, Qurban Ali, Hakim Manghwar
Publikováno v:
Plant Stress, Vol 14, Iss , Pp 100650- (2024)
Plants are incessantly challenged by a plethora of plant pests and pathogens, putting global agricultural productivity and food security at stake. Over several decades, various strategies have been developed in agriculture to overcome plant diseases
Externí odkaz:
https://doaj.org/article/dd3044b3c4e247e9836d5e1801360aa6
Autor:
Evan W. Kleinboehl, Kanut Laoharawee, Walker S. Lahr, Jacob D. Jensen, Joseph J. Peterson, Jason B. Bell, Beau R. Webber, Branden S. Moriarity
Publikováno v:
Molecular Therapy: Methods & Clinical Development, Vol 32, Iss 2, Pp 101253- (2024)
CRISPR-Cas9 and novel cas fusion proteins leveraging specific DNA targeting ability combined with deaminases or reverse transcriptases have revolutionized genome editing. However, their efficacy heavily relies upon protein variants, targeting single
Externí odkaz:
https://doaj.org/article/16649dec265345ac871b9882d548f54a
Publikováno v:
Molecular Therapy: Nucleic Acids, Vol 35, Iss 1, Pp 102138- (2024)
Programmable genome insertion (or knock-in) is vital for both fundamental and translational research. The continuously expanding number of CRISPR-based genome insertion strategies demonstrates the ongoing development in this field. Common methods for
Externí odkaz:
https://doaj.org/article/828f5721e0e64867a6a78a6056125f68
Publikováno v:
Frontiers in Genetics, Vol 15 (2024)
Externí odkaz:
https://doaj.org/article/df78bf9029d447859fd4e1e6b4d1d1bf
Publikováno v:
Rice Science, Vol 30, Iss 6, Pp 509-522 (2023)
Clustered regularly interspaced palindromic repeats (CRISPR)/CRISPR-associated protein (Cas)-mediated genome editing has greatly accelerated progress in plant genetic research and agricultural breeding by enabling targeted genomic modifications. More
Externí odkaz:
https://doaj.org/article/49561a514f0c4e4d827ebd9f5f1d00cc
Autor:
Carlye Frisch, William W. Kostes, Brooke Galyon, Brycelyn Whitman, Stefan J. Tekel, Kylie Standage-Beier, Gayathri Srinivasan, Xiao Wang, David A. Brafman
Publikováno v:
Molecular Therapy: Nucleic Acids, Vol 33, Iss , Pp 483-492 (2023)
Prime editing technologies enable precise genome editing without the caveats of CRISPR nuclease-based methods. Nonetheless, current approaches to identify and isolate prime-edited cell populations are inefficient. Here, we established a fluorescence-
Externí odkaz:
https://doaj.org/article/fd581bdbd382411aa0294ad4dbaae904