The population context is a driver of the heterogeneous response of epithelial cells to interferons

Autor: Camila Metz-Zumaran, Zina M Uckeley, Patricio Doldan, Francesco Muraca, Yagmur Keser, Pascal Lukas, Benno Kuropka, Leonie Küchenhoff, Soheil Rastgou Talemi, Thomas Höfer, Christian Freund, Elisabetta Ada Cavalcanti-Adam, Frederik Graw, Megan Stanifer, Steeve Boulant

Publikováno v: Molecular Systems Biology, Vol 20, Iss 3, Pp 242-275 (2024)

Abstract Isogenic cells respond in a heterogeneous manner to interferon. Using a micropatterning approach combined with high-content imaging and spatial analyses, we characterized how the population context (position of a cell with respect to neighbo

Externí odkaz: https://doaj.org/article/b6ad29bab90d41f698497d6990489043

Single‐cell analysis of population context advances RNAi screening at multiple levels

Autor: Berend Snijder, Raphael Sacher, Pauli Rämö, Prisca Liberali, Karin Mench, Nina Wolfrum, Laura Burleigh, Cameron C Scott, Monique H Verheije, Jason Mercer, Stefan Moese, Thomas Heger, Kristina Theusner, Andreas Jurgeit, David Lamparter, Giuseppe Balistreri, Mario Schelhaas, Cornelis A M De Haan, Varpu Marjomäki, Timo Hyypiä, Peter J M Rottier, Beate Sodeik, Mark Marsh, Jean Gruenberg, Ali Amara, Urs Greber, Ari Helenius, Lucas Pelkmans

Publikováno v: Molecular Systems Biology, Vol 8, Iss 1, Pp 1-18 (2012)

Abstract Isogenic cells in culture show strong variability, which arises from dynamic adaptations to the microenvironment of individual cells. Here we study the influence of the cell population context, which determines a single cell's microenvironme

Externí odkaz: https://doaj.org/article/099ab570f30a48879d45bf21e977d966

Phi-score: A cell-to-cell phenotypic scoring method for sensitive and selective hit discovery in cell-based assays

Autor: Ricky Bhajun, Stéphanie Combe, Jean-Philippe Vert, Eric Sulpice, J. Pablo Radicella, Philippe Rouillier, Christian Lajaunie, Mélissa Mary, Xavier Gidrol, Anna Campalans, Frédéric Fer, Guillaume Pinna, Patricia Obeid, Laurent Guyon

Publikováno v: Scientific Reports
Scientific Reports, Nature Publishing Group, 2015, 5 (14221), 〈http://www.nature.com/articles/srep14221〉. 〈10.1038/srep14221〉
Scientific Reports, 2015, 5 (14221), ⟨10.1038/srep14221⟩
Scientific Reports, Nature Publishing Group, 2015, 5 (14221), ⟨10.1038/srep14221⟩
Scientific Reports, Nature Publishing Group, 2015, 5 (1), ⟨10.1038/srep14221⟩

Phenotypic screening monitors phenotypic changes induced by perturbations, including those generated by drugs or RNA interference. Currently-used methods for scoring screen hits have proven to be problematic, particularly when applied to physiologica

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0ce96910220df677d60fd3185814a080
https://hal.archives-ouvertes.fr/hal-01246683/document

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