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Probing the 3D architecture of the plant nucleus with microscopy approaches: challenges and solutions

Autor: Tao Dumur, Susan Duncan, Katja Graumann, Sophie Desset, Ricardo S Randall, Ortrun Mittelsten Scheid, Hank W Bass, Dimiter Prodanov, Christophe Tatout, Célia Baroux

Publikováno v: Nucleus, Vol 10, Iss 1, Pp 181-212 (2019)

The eukaryotic cell nucleus is a central organelle whose architecture determines genome function at multiple levels. Deciphering nuclear organizing principles influencing cellular responses and identity is a timely challenge. Despite many similaritie

Externí odkaz: https://doaj.org/article/8696bbb78324474c95f0bd11ea24f3f8

Analysis of Lotus japonicus nuclear pore NUP107-160 subcomplex mutants reveals pronounced structural plasticity and functional redundancy

Autor: Andreas eBinder, Martin eParniske

Publikováno v: Frontiers in Plant Science, Vol 4 (2014)

Mutations in the Lotus japonicus nucleoporin genes, NUP85, NUP133 and NENA (SEH1), lead to defects in plant-microbe symbiotic signaling. The homologous proteins in yeast and vertebrates are part of the conserved NUP84/NUP107-160 sub-complex, which is

Externí odkaz: https://doaj.org/article/2081d790cf96441fad46e093e024952d

Deep learning for nuclear image analysis and application to 3D plant nuclei

Autor: Mougeot, Guillaume, Chausse, Frédéric, Desset, Sophie, Graumann, Katja

Publikováno v: ORASIS 2021
ORASIS 2021, Centre National de la Recherche Scientifique [CNRS], Sep 2021, Saint Ferréol, France

International audience; The continuous development of microscopy has led biologists to have access to large high-resolution 2D and 3D image datasets. Automatic analysis of cellular and nuclear images has become an important challenge in the bioimagin

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=dedup_wf_001::106de60e256dbefeefd8c764857a2d33
https://hal.science/hal-03339633

Is the plant nucleus a mechanical rheostat?

Autor: Rituparna Goswami, Marie-Edith Chabouté, Atef Asnacios, Olivier Hamant

Publikováno v: Current Opinion in Plant Biology
Current Opinion in Plant Biology, Elsevier, 2020, 57, pp.155-163. ⟨10.1016/j.pbi.2020.09.001⟩
Current Opinion in Plant Biology, 2020, 57, pp.155-163. ⟨10.1016/j.pbi.2020.09.001⟩

International audience; Beyond its biochemical nature, the nucleus is also a physical object. There is accumulating evidence that its mechanics plays a key role in gene expression, cytoskeleton organization, and more generally in cell and development

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8c1cd00c80d69a6e3aca4fc3110a8f0e
https://pubmed.ncbi.nlm.nih.gov/33128898