Sequential Genome Editing and Induced Excision of the Transgene in N. tabacum BY2 Cells

Autor: Maor Sheva, Uri Hanania, Tami Ariel, Albina Turbovski, Vishal Kumar Rameshchandra Rathod, Dina Oz, Yoram Tekoah, Yoseph Shaaltiel

Publikováno v: Frontiers in Plant Science, Vol 11 (2020)

While plant cells in suspension are becoming a popular platform for expressing biotherapeutic proteins, the need to pre-engineer these cells to better comply with their role as host cell lines is emerging. Heterologous DNA and selectable markers are

Externí odkaz: https://doaj.org/article/658f67c60f784860bcf490b63026a503

Establishment of a tobacco BY2 cell line devoid of plant-specific xylose and fucose as a platform for the production of biotherapeutic proteins

Autor: Yoseph Shaaltiel, Yehuda Gubbay, Yoram Tekoah, Mara Weiss, Albina Turbovski, Dina Oz, Liat Fux, Maor Sheva, Yehava Forster, Tami Ariel, Yaniv Azulay, Uri Hanania

Publikováno v: Plant Biotechnology Journal

Summary Plant‐produced glycoproteins contain N‐linked glycans with plant‐specific residues of β(1,2)‐xylose and core α(1,3)‐fucose, which do not exist in mammalian‐derived proteins. Although our experience with two enzymes that are used

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::08172e80ef2fe41470cb46e101a45b04
https://doi.org/10.1111/pbi.12702

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Different site-specific N-glycan types in wheat (Triticum aestivum L.) PAP phytase

Autor: Malene Jørgensen, Karen G. Welinder, Henrik Brinch-Pedersen, Giuseppe Dionisio

Publikováno v: Dionisio, G, Brinch-Pedersen, H, Welinder, K G & Jørgensen, M 2011, ' Different site-specific N-glycan types in wheat ( Triticum aestivum L.) PAP phytase ', Phytochemistry, vol. 72, no. 10, pp. 1173-1179 . https://doi.org/10.1016/j.phytochem.2010.12.023
Dionisio, G, Brinch-Pedersen, H, Welinder, K G & Jørgensen, M 2011, ' Different site-specific N-glycan types in wheat (Triticum aestivum L.) PAP phytase ', Phytochemistry, vol. 72, no. 10, pp. 1173-1179 . https://doi.org/10.1016/j.phytochem.2010.12.023

Phytase activity in grain is essential to make phosphate available to cell metabolism, and in food and feed. Cereals contain the purple acid phosphatase type of phytases (PAPhy). Mature wheat grain is dominated by TaPAPhy_a which, in the present work

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4625b11d7bb5a7017d1e3127406160af
https://pure.au.dk/ws/files/37807026/sdarticle.pdf

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