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Autor:
Elias M Puchner, Dushyant Mehra
Publikováno v:
Bio-Protocol, Vol 13, Iss 21 (2023)
Externí odkaz:
https://doaj.org/article/126cbcf9b0514012b0e22e465b2b3b8b
Autor:
Dushyant Mehra, Elias M Puchner
Publikováno v:
Bio-Protocol, Vol 13, Iss 20 (2023)
A fundamental understanding of gene regulation requires a quantitative characterization of the spatial organization and dynamics of chromatin. The advent of fluorescence super-resolution microscopy techniques such as photoactivated localization micro
Externí odkaz:
https://doaj.org/article/6827a8a24a4a4093bc2af2f94dcb4d69
Autor:
Mehra D; School of Physics and Astronomy, University of Minnesota, Twin Cities, MN, USA.; Department of Physiology and Biomedical Engineering, Mayo Clinic, Rochester, MN, USA., Pucher EM; School of Physics and Astronomy, University of Minnesota, Twin Cities, MN, USA.
Publikováno v:
Bio-protocol [Bio Protoc] 2023 Nov 05; Vol. 13 (21), pp. e4896. Date of Electronic Publication: 2023 Nov 05 (Print Publication: 2023).
Autor:
Zhu, Yina, Li, Pingchuan, Beuzer, Paolo, Tong, Zhisong, Watters, Robin, Lv, Dan, Murre, Cornelis, Cang, Hu
We demonstrate that endonuclease deficient Clustered Regularly Interspaced Short Palindromic Repeats CRISPR-associated Cas9 protein (dCas9) fused to the photo-convertible fluorescence protein monomeric mEos3.1 (dCas9-mEos3) can be used to resolve sub
Externí odkaz:
http://arxiv.org/abs/1403.6738
Publikováno v:
Proceedings of the National Academy of Sciences of the United States of America, 2012 Oct . 109(43), 17436-17441.
Externí odkaz:
https://www.jstor.org/stable/41829688
Autor:
Karathanasis C; Institute for Physical and Theoretical Chemistry, Goethe-University Frankfurt, Frankfurt, Germany., Sanchez CP; Center of Infectious Diseases, Parasitology, Universitätsklinikum Heidelberg, Heidelberg, Germany., Heilemann M; Institute for Physical and Theoretical Chemistry, Goethe-University Frankfurt, Frankfurt, Germany.; BioQuant-Center for Quantitative Biology, Heidelberg University, Heidelberg, Germany., Lanzer M; Center of Infectious Diseases, Parasitology, Universitätsklinikum Heidelberg, Heidelberg, Germany. michael.lanzer@med.uni-heidelberg.de.
Publikováno v:
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2022; Vol. 2470, pp. 457-466.
Publikováno v:
Methods in molecular biology (Clifton, N.J.). 2470
Super-resolution microscopy in the form of photoactivated localization microscopy (PALM) offers the possibility of counting single molecules in a cell, a cellular compartment or a molecular complex. PALM can, therefore, underpin molecular and biochem
Publikováno v:
Bio-Protocol, Vol 6, Iss 3 (2016)
RNA polymerase II (RNAPII) is the enzyme transcribing most of the eukaryotic protein-coding genes. Analysing the distribution and quantification of RNAPII can help understanding its function in interphase nuclei. Although several investigations in ma
Externí odkaz:
https://doaj.org/article/78e441e1a3ac486fadd54ba697d588d6
Publikováno v:
PLoS ONE. Dec2012, Vol. 7 Issue 12, p1-15. 15p.