Identification of a family of peptidoglycan transpeptidases reveals that Clostridioides difficile requires noncanonical cross-links for viability.

Autor: Bollinger KW; Department of Microbiology and Immunology, Carver College of Medicine, University of Iowa, Iowa City, IA 52242., Müh U; Department of Microbiology and Immunology, Carver College of Medicine, University of Iowa, Iowa City, IA 52242., Ocius KL; Department of Chemistry, University of Virginia, Charlottesville, VA 22904., Apostolos AJ; Department of Chemistry, University of Virginia, Charlottesville, VA 22904., Pires MM; Department of Chemistry, University of Virginia, Charlottesville, VA 22904., Helm RF; Department of Biochemistry, Virginia Tech, Blacksburg, VA 24061., Popham DL; Department of Biological Sciences, Virginia Tech, Blacksburg, VA 24061., Weiss DS; Department of Microbiology and Immunology, Carver College of Medicine, University of Iowa, Iowa City, IA 52242.; Graduate Program in Genetics, University of Iowa, Iowa City, IA 52242., Ellermeier CD; Department of Microbiology and Immunology, Carver College of Medicine, University of Iowa, Iowa City, IA 52242.; Graduate Program in Genetics, University of Iowa, Iowa City, IA 52242.

Publikováno v: Proceedings of the National Academy of Sciences of the United States of America [Proc Natl Acad Sci U S A] 2024 Aug 20; Vol. 121 (34), pp. e2408540121. Date of Electronic Publication: 2024 Aug 16.

Reduced peptidoglycan synthesis capacity impairs growth of E. coli at high salt concentration.

Autor: Alodaini D; Institute of Microbiology and Infection, School of Biosciences, University of Birmingham, Birmingham, United Kingdom., Hernandez-Rocamora V; Biosciences Institute, Faculty of Medical Sciences, Newcastle University, Newcastle upon Tyne, United Kingdom., Boelter G; Institute of Microbiology and Infection, School of Biosciences, University of Birmingham, Birmingham, United Kingdom., Ma X; Institute of Microbiology and Infection, School of Biosciences, University of Birmingham, Birmingham, United Kingdom., Alao MB; Institute of Microbiology and Infection, School of Biosciences, University of Birmingham, Birmingham, United Kingdom., Doherty HM; Institute of Microbiology and Infection, School of Biosciences, University of Birmingham, Birmingham, United Kingdom., Bryant JA; Institute of Microbiology and Infection, School of Biosciences, University of Birmingham, Birmingham, United Kingdom.; School of Life Sciences, University of Nottingham, Nottingham, United Kingdom., Moynihan P; Institute of Microbiology and Infection, School of Biosciences, University of Birmingham, Birmingham, United Kingdom., Moradigaravand D; KAUST Computational Bioscience Research Center, King Abdullah University of Science and Technology, Thuwal, Makkah, Saudi Arabia.; Laboratory for Infectious Disease Epidemiology, KAUST Smart-Health Initiative and Biological and Environmental Science and Engineering Division, King Abdullah University of Science and Technology, Thuwal, Makkah, Saudi Arabia., Glinkowska M; Department of Bacterial Molecular Genetics, Faculty of Biology, University of Gdańsk, Gdańsk, Poland., Vollmer W; Biosciences Institute, Faculty of Medical Sciences, Newcastle University, Newcastle upon Tyne, United Kingdom.; Institute for Molecular Bioscience, University of Queensland, Brisbane, Australia., Banzhaf M; Institute of Microbiology and Infection, School of Biosciences, University of Birmingham, Birmingham, United Kingdom.; Biosciences Institute, Faculty of Medical Sciences, Newcastle University, Newcastle upon Tyne, United Kingdom.

Publikováno v: MBio [mBio] 2024 Apr 10; Vol. 15 (4), pp. e0032524. Date of Electronic Publication: 2024 Mar 01.

Probing Peptidoglycan Synthesis in the Gut Commensal Akkermansia Muciniphila with Bioorthogonal Chemical Reporters.

Autor: Sminia TJ; Laboratory of Organic Chemistry, Wageningen University and Research, Wageningen, The, Netherlands., Aalvink S; Laboratory of Microbiology, Wageningen University and Research, Wageningen, The, Netherlands., de Jong H; Department of Chemical Biology and Drug Discovery, Utrecht Institute for Pharmaceutical Sciences and Bijvoet Center for Biomolecular Research, Utrecht University, Utrecht, The, Netherlands., Tempelaars MH; Laboratory of Food Microbiology, Wageningen University and Research, Wageningen, The, Netherlands., Zuilhof H; Laboratory of Organic Chemistry, Wageningen University and Research, Wageningen, The, Netherlands.; School of Pharmaceutical Science and Technology, Tianjin University, Tianjin, P.R. China., Abee T; Laboratory of Food Microbiology, Wageningen University and Research, Wageningen, The, Netherlands., de Vos WM; Laboratory of Microbiology, Wageningen University and Research, Wageningen, The, Netherlands.; Human Microbiome Research Program, Faculty of Medicine, University of Helsinki, Helsinki, Finland., Tytgat HLP; Laboratory of Microbiology, Wageningen University and Research, Wageningen, The, Netherlands.; Current address: Nestlé Institute of Health Sciences, Nestlé Research, Lausanne, Switzerland., Wennekes T; Laboratory of Organic Chemistry, Wageningen University and Research, Wageningen, The, Netherlands.; Department of Chemical Biology and Drug Discovery, Utrecht Institute for Pharmaceutical Sciences and Bijvoet Center for Biomolecular Research, Utrecht University, Utrecht, The, Netherlands.

Publikováno v: Chembiochem : a European journal of chemical biology [Chembiochem] 2024 Oct 01; Vol. 25 (19), pp. e202400037. Date of Electronic Publication: 2024 Jun 21.

A Time-Resolved FRET Assay Identifies a Small Molecule that Inhibits the Essential Bacterial Cell Wall Polymerase FtsW.

Autor: Park Y; Department of Microbiology, Harvard Medical School, 4 Blackfan Circle, Boston, MA-02115, USA.; Department of Chemistry and Chemical Biology, Harvard University, 12 Oxford St., Cambridge, MA-02138, USA., Taguchi A; Department of Microbiology, Harvard Medical School, 4 Blackfan Circle, Boston, MA-02115, USA.; Current address: SANKEN, The Institute of Scientific and Industrial Research), Osaka University, 8-1 Mihogaoka, Ibaraki, Osaka, 567-0047, Japan., Baidin V; Department of Chemistry and Chemical Biology, Harvard University, 12 Oxford St., Cambridge, MA-02138, USA., Kahne D; Department of Chemistry and Chemical Biology, Harvard University, 12 Oxford St., Cambridge, MA-02138, USA., Walker S; Department of Microbiology, Harvard Medical School, 4 Blackfan Circle, Boston, MA-02115, USA.

Publikováno v: Angewandte Chemie (International ed. in English) [Angew Chem Int Ed Engl] 2023 Jun 19; Vol. 62 (25), pp. e202301522. Date of Electronic Publication: 2023 May 11.

Two codependent routes lead to high-level MRSA.

Autor: Adedeji-Olulana AF; School of Mathematical and Physical Sciences, University of Sheffield, Sheffield, UK., Wacnik K; School of Biosciences, University of Sheffield, Sheffield, UK.; The Florey Institute, University of Sheffield, Sheffield, UK., Lafage L; School of Biosciences, University of Sheffield, Sheffield, UK.; The Florey Institute, University of Sheffield, Sheffield, UK., Pasquina-Lemonche L; School of Mathematical and Physical Sciences, University of Sheffield, Sheffield, UK.; The Florey Institute, University of Sheffield, Sheffield, UK., Tinajero-Trejo M; School of Biosciences, University of Sheffield, Sheffield, UK.; The Florey Institute, University of Sheffield, Sheffield, UK., Sutton JAF; School of Biosciences, University of Sheffield, Sheffield, UK.; The Florey Institute, University of Sheffield, Sheffield, UK., Bilyk B; School of Biosciences, University of Sheffield, Sheffield, UK.; The Florey Institute, University of Sheffield, Sheffield, UK., Irving SE; School of Biosciences, University of Sheffield, Sheffield, UK.; The Florey Institute, University of Sheffield, Sheffield, UK., Portman Ross CJ; School of Biosciences, University of Sheffield, Sheffield, UK.; The Florey Institute, University of Sheffield, Sheffield, UK., Meacock OJ; School of Mathematical and Physical Sciences, University of Sheffield, Sheffield, UK., Randerson SA; School of Mathematical and Physical Sciences, University of Sheffield, Sheffield, UK., Beattie E; School of Mathematical and Physical Sciences, University of Sheffield, Sheffield, UK., Owen DS; School of Biosciences, University of Sheffield, Sheffield, UK., Florence J; School of Biosciences, University of Sheffield, Sheffield, UK.; The Florey Institute, University of Sheffield, Sheffield, UK., Durham WM; School of Mathematical and Physical Sciences, University of Sheffield, Sheffield, UK., Hornby DP; School of Biosciences, University of Sheffield, Sheffield, UK.; The Florey Institute, University of Sheffield, Sheffield, UK., Corrigan RM; School of Biosciences, University of Sheffield, Sheffield, UK.; The Florey Institute, University of Sheffield, Sheffield, UK.; School of Medicine, University College Dublin, Dublin, Ireland., Green J; School of Biosciences, University of Sheffield, Sheffield, UK.; The Florey Institute, University of Sheffield, Sheffield, UK., Hobbs JK; School of Mathematical and Physical Sciences, University of Sheffield, Sheffield, UK., Foster SJ; School of Biosciences, University of Sheffield, Sheffield, UK.; The Florey Institute, University of Sheffield, Sheffield, UK.

Publikováno v: Science (New York, N.Y.) [Science] 2024 Nov; Vol. 386 (6721), pp. 573-580. Date of Electronic Publication: 2024 Oct 31.