Zobrazeno 1 - 10
of 306
pro vyhledávání: '"pSC101"'
Publikováno v:
International Journal of Molecular Sciences
International Journal of Molecular Sciences, Vol 22, Iss 8886, p 8886 (2021)
Volume 22
Issue 16
International Journal of Molecular Sciences, Vol 22, Iss 8886, p 8886 (2021)
Volume 22
Issue 16
The Hfq protein is a bacterial RNA chaperone, involved in many molecular interactions, including control of actions of various small RNA regulatory molecules. We found that the presence of Hfq was required for survival of plasmid-containing Escherich
Publikováno v:
Plasmid
Readily curable plasmids facilitate the construction of plasmid-free bacterial strains after the plasmid encoded genes are no longer needed. The most popular of these plasmids features a temperature-sensitive (Ts) pSC101 origin of replication which c
Publikováno v:
Proceedings of the National Academy of Sciences of the United States of America, 1986 Jun . 83(12), 4423-4427.
Externí odkaz:
https://www.jstor.org/stable/27991
Publikováno v:
Biotechnology and Bioengineering. 113:2064-2071
With the recent revival of the promise of plasmid DNA vectors in gene therapy, a novel synthetic biology approach was used to enhance the quantity, (yield), and quality of the plasmid DNA. Quality was measured by percentage supercoiling and supercoil
Autor:
Maren Wehrs, Aindrila Mukhopadhyay, Jay D. Keasling, Nima Sedaghatian, Jesus F. Barajas, Mitchell G. Thompson, Constance B. Bailey, Nathan J. Hillson, Nurgul Kaplan
Publikováno v:
Thompson, MG; Sedaghatian, N; Barajas, JF; Wehrs, M; Bailey, CB; Kaplan, N; et al.(2018). Isolation and characterization of novel mutations in the pSC101 origin that increase copy number. Scientific Reports, 8(1). doi: 10.1038/s41598-018-20016-w. UC Berkeley: Retrieved from: http://www.escholarship.org/uc/item/6c13b5hr
Scientific reports, vol 8, iss 1
Thompson, M G, Sedaghatian, N, Barajas, J F, Wehrs, M, Bailey, C B, Kaplan, N, Hillson, N J, Mukhopadhyay, A & Keasling, J D 2018, ' Isolation and characterization of novel mutations in the pSC101 origin that increase copy number ', Scientific Reports, vol. 8, 1590 . https://doi.org/10.1038/s41598-018-20016-w
Scientific Reports, Vol 8, Iss 1, Pp 1-11 (2018)
Scientific Reports
Scientific reports, vol 8, iss 1
Thompson, M G, Sedaghatian, N, Barajas, J F, Wehrs, M, Bailey, C B, Kaplan, N, Hillson, N J, Mukhopadhyay, A & Keasling, J D 2018, ' Isolation and characterization of novel mutations in the pSC101 origin that increase copy number ', Scientific Reports, vol. 8, 1590 . https://doi.org/10.1038/s41598-018-20016-w
Scientific Reports, Vol 8, Iss 1, Pp 1-11 (2018)
Scientific Reports
pSC101 is a narrow host range, low-copy plasmid commonly used for genetically manipulating Escherichia coli. As a byproduct of a genetic screen for a more sensitive lactam biosensor, we identified multiple novel mutations that increase the copy numbe
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::025b4ef9b5743466c442309c555b1a3f
http://www.escholarship.org/uc/item/6c13b5hr
http://www.escholarship.org/uc/item/6c13b5hr
Autor:
Paulo Lee Ho, Celso Raul Romero Ramos, Viviane Maimoni Gonçalves, Eneas Carvalho, Henrique Roman Ramos, Daniel Omote, Stefanie Kraschowetz, Fara A. P. Eguia
Publikováno v:
Plasmid. 98
Expression vectors for industrial production should be stable and allow tight control of protein synthesis. This is necessary to ensure plasmid transmission to daughter cells in order to achieve a stable population capable of synthesizing high amount
Autor:
Yasurou Kurusu, Hiroe Hayashi
Publikováno v:
Microbes and Environments. 29:96-99
We have identified and analyzed two DNA regions responsible for stable maintenance of a plasmid in the genus Sphingomonas and Escherichia coli. A 37 bp fragment, upstream of the repA gene, is required for stable maintenance of the low-copy-number sma
Autor:
Hayashi, Hiroe, Kurusu, Yasurou
Publikováno v:
Microbes and Environments
We have identified and analyzed two DNA regions responsible for stable maintenance of a plasmid in the genus Sphingomonas and Escherichia coli. A 37 bp fragment, upstream of the repA gene, is required for stable maintenance of the low-copy-number sma
Publikováno v:
Plasmid. 70:353-361
Plasmid loss rate measurements are standard in microbiology and key to understanding plasmid stabilization mechanisms. The conventional assays eliminate selection for plasmids at the beginning of the experiment and screen for the appearance of plasmi
Akademický článek
Tento výsledek nelze pro nepřihlášené uživatele zobrazit.
K zobrazení výsledku je třeba se přihlásit.
K zobrazení výsledku je třeba se přihlásit.