Preparation of oxygen-sensitive proteins for high-resolution cryoEM structure determination using (an)aerobic blot-free vitrification.

Autor: Cook BD; Department of Chemistry and Biochemistry, University of California, San Diego, California, USA., Narehood SM; Department of Chemistry and Biochemistry, University of California, San Diego, California, USA., McGuire KL; Department of Chemistry and Biochemistry, University of California, San Diego, California, USA., Li Y; Department of Chemistry and Biochemistry, University of California, San Diego, California, USA., Tezcan FA; Department of Chemistry and Biochemistry, University of California, San Diego, California, USA., Herzik MA Jr; Department of Chemistry and Biochemistry, University of California, San Diego, California, USA.

Publikováno v: BioRxiv : the preprint server for biology [bioRxiv] 2024 Jul 22. Date of Electronic Publication: 2024 Jul 22.

Hydrogen Deuterium Exchange Mass Spectrometry of Oxygen Sensitive Proteins.

Autor: Berry L; Department of Chemistry and Biochemistry, Montana State University, Bozeman, USA., Patterson A; Department of Chemistry and Biochemistry, Montana State University, Bozeman, USA., Pence N; Institute of Biological Chemistry, Washington State University, Pullman, USA., Peters JW; Institute of Biological Chemistry, Washington State University, Pullman, USA., Bothner B; Department of Chemistry and Biochemistry, Montana State University, Bozeman, USA.

Publikováno v: Bio-protocol [Bio Protoc] 2018 Mar 20; Vol. 8 (6).

A miniature airlock system to aid the cryo-cooling of protein crystals grown under anoxic conditions

Autor: Peter van der Linden, Sylvain Engilberge, Mohamed Atta, Philippe Carpentier

Publikováno v: Journal of Applied Crystallography
Journal of Applied Crystallography, 2022, 55 (5), pp.1212-1218. ⟨10.1107/S1600576722007580⟩

Crystallographic studies of structures and mechanisms of dioxygen-employing enzymes or oxygen-sensitive proteins usually require these molecules to be prepared and crystallized under anoxic conditions in gloveboxes. Handling, cryo-cooling and extract

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4fc86cfbb4d517693b6f3f7c9b335626
https://doi.org/10.1107/s1600576722007580

Tento výsledek nelze pro nepřihlášené uživatele zobrazit.
K zobrazení výsledku je třeba se přihlásit.

Adaptation of the GoldenBraid modular cloning system and creation of a toolkit for the expression of heterologous proteins in yeast mitochondria.

Autor: Pérez-González, Ana¹, Kniewel, Ryan^1,2, Veldhuizen, Marcel¹, Verma, Hemant K.^1,3, Navarro-Rodríguez, Mónica¹, Rubio, Luis M.¹, Caro, Elena¹ elena.caro@upm.es

Publikováno v: BMC Biotechnology. 11/13/2017, Vol. 17, p1-11. 11p.