Zobrazeno 1 - 10
of 439
pro vyhledávání: '"off-target effect"'
Publikováno v:
Scientific Reports, Vol 14, Iss 1, Pp 1-12 (2024)
Abstract The protein kinases DYRK1A and DYRK1B are pivotal regulators of cell cycle progression by promoting cell cycle exit into quiescence. DYRK1B appears to play a more important role in cancer cell quiescence than DYRK1A, as evidenced by its over
Externí odkaz:
https://doaj.org/article/935b43de979842cb9b78a406a220ef31
Autor:
Asmamaw Mengstie M, Teshome Azezew M, Asmamaw Dejenie T, Teshome AA, Tadele Admasu F, Behaile Teklemariam A, Tilahun Mulu A, Mekonnen Agidew M, Adugna DG, Geremew H, Abebe EC
Publikováno v:
Biologics: Targets & Therapy, Vol Volume 18, Pp 21-28 (2024)
Misganaw Asmamaw Mengstie,1 Muluken Teshome Azezew,2 Tadesse Asmamaw Dejenie,3 Assefa Agegnehu Teshome,4 Fitalew Tadele Admasu,1 Awgichew Behaile Teklemariam,1 Anemut Tilahun Mulu,1 Melaku Mekonnen Agidew,1 Dagnew Getnet Adugna,5 Habtamu Geremew,6 En
Externí odkaz:
https://doaj.org/article/587bd3dceb1f45f4978d22fae6ad7b32
Publikováno v:
Advanced Science, Vol 11, Iss 5, Pp n/a-n/a (2024)
Abstract Base editors, which enable targeted locus nucleotide conversion in genomic DNA without double‐stranded breaks, have been engineered as powerful tools for biotechnological and clinical applications. However, the application of base editors
Externí odkaz:
https://doaj.org/article/e921c7db78af42559b0cf889ff3ec837
Autor:
Atsushi Shibata, Hisao Shirohzu, Yusuke Iwakami, Tomoaki Abe, Chisato Emura, Eriko Aoki, Tadaaki Ohgi
Publikováno v:
Molecular Therapy: Nucleic Acids, Vol 32, Iss , Pp 468-477 (2023)
Small interfering RNA (siRNA) and short hairpin RNA (shRNA) are widely used as RNA interference (RNAi) reagents. Recently, truncated shRNAs that trigger RNAi in a Dicer-independent manner have been developed. We generated a novel class of RNAi reagen
Externí odkaz:
https://doaj.org/article/7fa797aaa0604488b979cd3b9dfb4ed7
Publikováno v:
Molecular Therapy: Nucleic Acids, Vol 34, Iss , Pp 102072- (2023)
Paired SpCas9 nickases (SpCas9n) are an effective strategy to reduce off-target effect in genome editing. However, this approach is not efficient with 3′-overhanging ends, limiting its applications. In order to expand the utility of paired SpCas9n
Externí odkaz:
https://doaj.org/article/8c880b1b54f84caeabaf083b01abb9ed
Autor:
Zhenwu Zhang, Wanyu Tao, Shisheng Huang, Wenjun Sun, Yue Wang, Wen Jiang, Xingxu Huang, Chao-Po Lin
Publikováno v:
Molecular Therapy: Nucleic Acids, Vol 29, Iss , Pp 502-510 (2022)
Genome editing in pluripotent stem cells (PSCs) using CRISPR technology holds great promise for therapeutic applications. Yet, it has been reported that Cas9-mediated cleavage could cause large deletions or rearrangements of DNA, and the selection of
Externí odkaz:
https://doaj.org/article/dc386a3b11744e218cfbac4f06ee4021
Autor:
Si-Cheng Liu, Yi-Li Feng, Xiu-Na Sun, Ruo-Dan Chen, Qian Liu, Jing-Jing Xiao, Jin-Na Zhang, Zhi-Cheng Huang, Ji-Feng Xiang, Guo-Qiao Chen, Yi Yang, Chao Lou, Hao-Dan Li, Zhen Cai, Shi-Ming Xu, Hui Lin, An-Yong Xie
Publikováno v:
Genome Biology, Vol 23, Iss 1, Pp 1-32 (2022)
Abstract Background Due to post-cleavage residence of the Cas9-sgRNA complex at its target, Cas9-induced DNA double-strand breaks (DSBs) have to be exposed to engage DSB repair pathways. Target interaction of Cas9-sgRNA determines its target binding
Externí odkaz:
https://doaj.org/article/3baeafdd016e47d9820a0acff3a8a5a0
Publikováno v:
Cellular & Molecular Biology Letters, Vol 27, Iss 1, Pp 1-28 (2022)
Abstract MicroRNAs (miRNAs), which were initially discovered in Caenorhabditis elegans, can regulate gene expression by recognizing cognate sequences and interfering with the transcriptional or translational machinery. The application of bioinformati
Externí odkaz:
https://doaj.org/article/354d4eefd42b49a3a4e8a8c5aafbf8e3
Autor:
Francesca Lofrumento, Natasha Irrera, Roberto Licordari, Silvia Perfetti, Enrica Nasso, Paolo Liotta, Giovanni Isgrò, Victoria Garcia-Ruiz, Francesco Squadrito, Scipione Carerj, Gianluca Di Bella, Antonio Micari, Francesco Costa
Publikováno v:
International Journal of Molecular Sciences, Vol 24, Iss 24, p 17546 (2023)
Several studies have demonstrated that, beyond their antithrombotic effects, P2Y12 receptor inhibitors may provide additional off-target effects through different mechanisms. These effects range from the preservation of endothelial barrier function t
Externí odkaz:
https://doaj.org/article/19afa3cb96cb441ea14c9f43d616f1f2
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