Zobrazeno 1 - 10
of 45
pro vyhledávání: '"ocis:(180.2520) Fluorescence microscopy"'
Publikováno v:
Optics Letters
We compare the performance of linear and nonlinear methods for aligning the excitation and detection planes throughout volumes of large specimens in digitally scanned light sheet microscopy. An effective nonlinear method involves the registration of
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bf621592a95d35d82835db6ebeaa8e50
https://www.repository.cam.ac.uk/handle/1810/273591
https://www.repository.cam.ac.uk/handle/1810/273591
Publikováno v:
Biomedical Optics Express
Light sheet fluorescence microscopy offers considerable potential to the cellular neuroscience community as it makes it possible to image extensive areas of neuronal structures, such as axons or dendrites, with a low light budget, thereby minimizing
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::0f333f2ee9315f81f2ccdc5dc82af2c7
https://pubmed.ncbi.nlm.nih.gov/29760977
https://pubmed.ncbi.nlm.nih.gov/29760977
Autor:
Kevin O'Holleran, Michael Shaw
Publikováno v:
Biomedical Optics Express
The use of structured illumination in fluorescence microscopy allows the suppression of out of focus light and an increase in effective spatial resolution. In this paper we consider different approaches for reconstructing 2D structured illumination i
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d0c7b433b765381ec3cf14365613645c
http://europepmc.org/articles/PMC4132990
http://europepmc.org/articles/PMC4132990
Publikováno v:
Optics Express
Coherent detection through two opposing objectives (4Pi configuration) improves the precision of three-dimensional (3D) single-molecule localization substantially along the axial direction, but suffers from instrument complexity and maintenance diffi
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::90d88cefef0f610856dfd4e87a00b49d
http://europepmc.org/articles/PMC3796685
http://europepmc.org/articles/PMC3796685
Publikováno v:
Biomedical Optics Express
We have investigated the effect of Airy illumination on the image quality and depth penetration of digitally scanned light-sheet microscopy in turbid neural tissue. We used Fourier analysis of images acquired using Gaussian and Airy light-sheets to a
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::0231f0b0baf9f8b2e960404c089ef03f
https://pubmed.ncbi.nlm.nih.gov/27867712
https://pubmed.ncbi.nlm.nih.gov/27867712
Publikováno v:
Biomedical Optics Express
We report on simultaneous frequency domain optical-resolution photoacoustic and fluorescence microscopy with sub-µm lateral resolution. With the help of a blood smear, we show that photoacoustic and fluorescence images provide complementary informat
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::b138adb4ef1f8e5f63fadff187ca91ac
https://pubmed.ncbi.nlm.nih.gov/27446698
https://pubmed.ncbi.nlm.nih.gov/27446698
Publikováno v:
Biomedical Optics Express
An optical method is presented that allows the measurement of the triplet lifetime of a fluorescent molecule. This is a characteristic specific to each fluorophore. Based on differences in triplet lifetimes of two fluorescent species (autofluorescenc
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a43d437c264b3d17d07ba9118c43bd75
http://europepmc.org/articles/PMC3469990
http://europepmc.org/articles/PMC3469990
Publikováno v:
Biomedical Optics Express
We demonstrate a fiber-based, three-color femtosecond source for simultaneous imaging of three fluorescent proteins (FPs) using two-photon fluorescence microscopy (2PM). The three excitation wavelengths at 775 nm, 864 nm and 950 nm, are obtained thro
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::16a3df578abc7ac4e713fbce3bc11e44
http://europepmc.org/articles/PMC3447541
http://europepmc.org/articles/PMC3447541
Publikováno v:
Biomedical Optics Express
Probing biological structures and functions deep inside live organisms with light is highly desirable. Among the current optical imaging modalities, multiphoton fluorescence microscopy exhibits the best contrast for imaging scattering samples by empl
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::33a3281b22f9fa11be17e51dd9114530
http://europepmc.org/articles/PMC3409713
http://europepmc.org/articles/PMC3409713
Autor:
Akiko Kumagai, Hideaki Mizuno, Akira Suda, Atsushi Miyawaki, Katsumi Midorikawa, Takanori Takeda, Keisuke Isobe, Hiroyuki Kawano
Publikováno v:
Biomedical Optics Express
ResearcherID
ResearcherID
We demonstrate how the resolution and imaging depth limitations of nonlinear optical microscopy can be overcome by modulating the spatial overlap between two-color pulses. We suppress out-of-focus signals, which limit the imaging depth, by a factor o
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::04ce27b6ee151aafb1701758d41ed6e8
http://europepmc.org/articles/PMC3395484
http://europepmc.org/articles/PMC3395484