High-Quality Nucleic Acid Isolation from Hard-to-Lyse Bacterial Strains Using PMAP-36, a Broad-Spectrum Antimicrobial Peptide

Autor: Munjeong Choi, Hyoim Jeon, Hye-sun Cho, Kwonho Hong, Jin-Hoi Kim, Chankyu Park, Yunjung Lee, Nagasundarapandian Soundrarajan, Byeongyong Ahn

Publikováno v: International Journal of Molecular Sciences
International Journal of Molecular Sciences, Vol 22, Iss 4149, p 4149 (2021)
Volume 22
Issue 8

The efficiency of existing cell lysis methods to isolate nucleic acids from diverse bacteria varies depending on cell wall structures. This study tested a novel idea of using broad-spectrum antimicrobial peptides to improve the lytic efficiency of ha

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6560557911fd8b2d68aba608cd5b56be
http://europepmc.org/articles/PMC8073543

Rapid, Safe, and Simple Manual Bedside Nucleic Acid Extraction for the Detection of Virus in Whole Blood Samples

Autor: Christopher E. Jensen, Anders Fomsgaard, Maiken Worsøe Rosenstierne

Publikováno v: J Vis Exp
Rosenstierne, M W, Jensen, C E & Fomsgaard, A 2018, ' Rapid, safe, and simple manual bedside nucleic acid extraction for the detection of virus in whole blood samples ', Journal of Visualized Experiments, vol. 2018, no. 136, e58001 . https://doi.org/10.3791/58001

The rapid diagnosis of an infection is essential for the outbreak management, risk containment, and patient care. We have previously shown a method for the rapid bedside inactivation of the Ebola virus during blood sampling for safe nucleic acid (NA)

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1c7386fffdb27846d2bf9cc675ee3dcc
https://doi.org/10.3791/58001-v

Multicentre validation study of nucleic acids extraction from FFPE tissues

Autor: Bozo Kruslin, Manfred Dietel, Patricia J. T. A. Groenen, Carsten Denkert, Paula Soares, Hannelore Kothmaier, Pedro L. Fernández, Giorgio Stanta, Aurel Perren, Helmuth Popper, Falk Hlubek, Chiara Maria Mazzanti, Serena Bonin, Jean Benhattar, Gerald Höfler, Aldo Scarpa

Publikováno v: Virchows Archiv
Virchows Archiv, 457, 3, pp. 309-17
Bonin, Serena; Hlubek, Falk; Benhattar, Jean; Denkert, Carsten; Dietel, Manfred; Fernandez, Pedro L; Höfler, Gerald; Kothmaier, Hannelore; Kruslin, Bozo; Mazzanti, Chiara Maria; Perren, Aurel; Popper, Helmuth; Scarpa, Aldo; Soares, Paula; Stanta, Giorgio; Groenen, Patricia J T A (2010). Multicentre validation study of nucleic acids extraction from FFPE tissues. Virchows Archiv, 457(3), pp. 309-17. Berlin: Springer 10.1007/s00428-010-0917-5
Virchows Archiv, 457, 309-17
Virchows Archiv : an international journal of pathology, vol. 457, no. 3, pp. 309-317

In most pathology laboratories worldwide, formalin-fixed paraffin embedded (FFPE) samples are the only tissue specimens available for routine diagnostics. Although commercial kits for diagnostic molecular pathology testing are becoming available, mos

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f147749b9178c16b5dd69183e7abdce3
https://www.bib.irb.hr/461811