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A primitive ventricle at embryonic day 8.0 - 9.5 composes of approximately a thousand cells. This protocol describes a pipeline to pool multiple snap-frozen or cryopreserved ventricles and isolate a large number of nuclei for single-nuclei RNAseq. Th
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::4303d77cd685612eb5bc1c18202de03c
https://doi.org/10.17504/protocols.io.bw6tphen
https://doi.org/10.17504/protocols.io.bw6tphen
A primitive ventricle at embryonic day 8.0 - 9.5 composes of approximately a thousand cells. This protocol describes a pipeline to pool multiple snap-frozen or cryopreserved ventricles and isolate a large number of nuclei for single-nuclei RNAseq. Th
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::dea0056582f929175be08e7bdd7c9f97
https://doi.org/10.17504/protocols.io.bniwmcfe
https://doi.org/10.17504/protocols.io.bniwmcfe
This protocol demonstrates the step-by-step procedure of the ventricle dissection and cryopreservation at early mouse embryonic days for the subsequent single-nuclei RNAseq pipeline. This method is suitable for samples harvested from the genetically
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::fc6cb3b24089f0d4ad8efa91a5a07238
https://doi.org/10.17504/protocols.io.bm7ck9iw
https://doi.org/10.17504/protocols.io.bm7ck9iw