Automated workflow for the cell cycle analysis of (non-)adherent cells using a machine learning approach

Autor: Kourosh Hayatigolkhatmi, Chiara Soriani, Emanuel Soda, Elena Ceccacci, Oualid El Menna, Sebastiano Peri, Ivan Negrelli, Giacomo Bertolini, Gian Martino Franchi, Roberta Carbone, Saverio Minucci, Simona Rodighiero

Publikováno v: eLife, Vol 13 (2024)

Understanding the cell cycle at the single-cell level is crucial for cellular biology and cancer research. While current methods using fluorescent markers have improved the study of adherent cells, non-adherent cells remain challenging. In this study

Externí odkaz: https://doaj.org/article/25ec1da52c74434895f994bf63041cdf

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Protocol for adhesion and immunostaining of lymphocytes and other non-adherent cells in culture

Autor: Matthew Tsang, Jennifer Gantchev, Feras M. Ghazawi, Ivan V. Litvinov

Publikováno v: BioTechniques, Vol 63, Iss 5, Pp 230-233 (2017)

Immunostaining of non-adherent cells is commonly performed after adhesion of cells onto microscope slides either using cytocentrifugation or with the help of charged coating substrates. These techniques, however, require either specialized equipment

Externí odkaz: https://doaj.org/article/4e85e2226fca4c09aaf6a9dbdfaf7493

Effect of Graphene Family Materials on Multiple Myeloma and Non-Hodgkin’s Lymphoma Cell Lines

Autor: Norma C. Gutiérrez, Barbara Strojny, Piotr Koczoń, Marta Grodzik, Irena Misiewicz-Krzeminska, Isabel M. Isidro, Sławomir Jaworski, Ewa Sawosz, André Chwalibog, Elizabeta A. Rojas

Publikováno v: Strojny, B, Jaworski, S, Misiewicz-Krzeminska, I, Isidro, I, Rojas, E A, Gutiérrez, N C, Grodzik, M, Koczoń, P, Chwalibog, A & Sawosz, E 2020, ' Effect of Graphene Family Materials on Multiple Myeloma and Non-Hodgkin’s Lymphoma Cell Lines ', Materials, vol. 13, no. 15, 3420 . https://doi.org/10.3390/ma13153420
Materials, Vol 13, Iss 3420, p 3420 (2020)
Strojny, B, Jaworski, S, Misiewicz-Krzemińska, I, Isidro, I, Rojas, E A, Gutiérrez, N C, Grodzik, M, Koczoń, P, Chwalibog, A & Sawosz, E 2020, ' Effect of graphene family materials on multiple myeloma and non-Hodgkin's lymphoma cell lines ', Materials, vol. 13, no. 15, 3420 . https://doi.org/10.3390/ma13153420
Materials
Volume 13
Issue 15

The interest around the graphene family of materials is constantly growing due to their potential application in biomedical fields. The effect of graphene and its derivatives on cells varies amongst studies depending on the cell and tissue type. Sinc

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1492f341b2b888447bb23b9ce2b533cf
https://curis.ku.dk/ws/files/245671849/Strojny_et_al_2020_materials_13_03420_v2.pdf

Optimization of proximity ligation assay (PLA) for detection of protein interactions and fusion proteins in non-adherent cells: application to pre-B lymphocytes

Autor: Lydie Debaize, Marie-Bérengère Troadec, Hélène Jakobczyk, Anne-Gaëlle Rio, Virginie Gandemer

Publikováno v: Molecular Cytogenetics
Molecular Cytogenetics, 2017, 10 (1), pp.27. ⟨10.1186/s13039-017-0328-2⟩
Molecular Cytogenetics, BioMed Central, 2017, 10 (1), pp.27. ⟨10.1186/s13039-017-0328-2⟩
Molecular Cytogenetics, Vol 10, Iss 1, Pp 1-13 (2017)

Background Genetic abnormalities, including chromosomal translocations, are described for many hematological malignancies. From the clinical perspective, detection of chromosomal abnormalities is relevant not only for diagnostic and treatment purpose

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::49a9dd9d0bb78d154db3ad79114006e2
https://pubmed.ncbi.nlm.nih.gov/28736577