Zobrazeno 1 - 10
of 342
pro vyhledávání: '"native gel electrophoresis"'
Autor:
Chiaki Sakuma, Masataka Nakagawa, Yui Tomioka, Toshiaki Maruyama, Kevin Entzminger, Jonathan K Fleming, Takashi Shibata, Yasunori Kurosawa, CJ Okumura, Tsutomu Arakawa, Teruo Akuta
Publikováno v:
BioTechniques, Vol 72, Iss 5, Pp 207-218 (2022)
We have developed a new Western blotting method of native proteins from agarose-based gel electrophoresis using a buffer at pH 6.1 containing basic histidine and acidic 2-(N-morpholino)ethanesulfonic acid. This gel electrophoresis successfully provid
Externí odkaz:
https://doaj.org/article/617c78fb8b3e44e2b265eac57674e82c
Akademický článek
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Blue Native PAGE–Antibody Shift in Conjunction with Mass Spectrometry to Reveal Protein Subcomplexes
Autor:
Miao Chen, Frank Koopmans, Iryna Paliukhovich, Sophie J. F. van der Spek, Jian Dong, August B. Smit, Ka Wan Li
Publikováno v:
International Journal of Molecular Sciences, 24(8):7632, 1-14. Multidisciplinary Digital Publishing Institute (MDPI)
International Journal of Molecular Sciences; Volume 24; Issue 8; Pages: 7632
Chen, M, Koopmans, F, Paliukhovich, I, van der Spek, S J F, Dong, J, Smit, A B & Li, K W 2023, ' Blue Native PAGE–Antibody Shift in Conjunction with Mass Spectrometry to Reveal Protein Subcomplexes : Detection of a Cerebellar α1/α6-Subunits Containing γ-Aminobutyric Acid Type A Receptor Subtype ', International Journal of Molecular Sciences, vol. 24, no. 8, 7632, pp. 1-14 . https://doi.org/10.3390/ijms24087632
International Journal of Molecular Sciences; Volume 24; Issue 8; Pages: 7632
Chen, M, Koopmans, F, Paliukhovich, I, van der Spek, S J F, Dong, J, Smit, A B & Li, K W 2023, ' Blue Native PAGE–Antibody Shift in Conjunction with Mass Spectrometry to Reveal Protein Subcomplexes : Detection of a Cerebellar α1/α6-Subunits Containing γ-Aminobutyric Acid Type A Receptor Subtype ', International Journal of Molecular Sciences, vol. 24, no. 8, 7632, pp. 1-14 . https://doi.org/10.3390/ijms24087632
The pentameric γ-Aminobutyric acid type A receptors (GABAARs) are ligand-gated ion channels that mediate the majority of inhibitory neurotransmission in the brain. In the cerebellum, the two main receptor subtypes are the 2α1/2β/γ and 2α6/2β/δ
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::729d1b2a87dfbdad83a37e28de13e056
https://research.vu.nl/en/publications/2a1fa5a0-e958-418e-ba0d-7902a5b01175
https://research.vu.nl/en/publications/2a1fa5a0-e958-418e-ba0d-7902a5b01175
Autor:
Fang Zhang, Steven B. Vik
Publikováno v:
BBA Advances, Vol 1, Iss , Pp 100027- (2021)
Respiratory Complex I, a multi-subunit, membrane-bound enzyme, oxidizes NADH in the electron transport chains of mammalian mitochondria, and many bacterial species. We have examined in vivo assembly of the membrane subunits of Complex I from E. coli.
Externí odkaz:
https://doaj.org/article/7d142670d6614caa86b0f1d26173b711
Akademický článek
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Autor:
Karabadzhak, Alexander G., Petti, Lisa M., Barrera, Francisco N., Edwards, Anne P. B., Moya-Rodríguez, Andrés, Polikanov, Yury S., Freites, J. Alfredo, Tobias, Douglas J., Engelman, Donald M., DiMaio, Daniel
Publikováno v:
Proceedings of the National Academy of Sciences of the United States of America, 2017 Aug . 114(35), E7262-E7271.
Externí odkaz:
https://www.jstor.org/stable/26487396
Autor:
Saara Tegelberg, Nikica Tomašić, Jukka Kallijärvi, Janne Purhonen, Eskil Elmér, Eva Lindberg, David Gisselsson Nord, Maria Soller, Nicole Lesko, Anna Wedell, Helene Bruhn, Christoph Freyer, Henrik Stranneheim, Rolf Wibom, Inger Nennesmo, Anna Wredenberg, Erik A. Eklund, Vineta Fellman
Publikováno v:
Orphanet Journal of Rare Diseases, Vol 12, Iss 1, Pp 1-14 (2017)
Abstract Background Mitochondrial diseases due to defective respiratory chain complex III (CIII) are relatively uncommon. The assembly of the eleven-subunit CIII is completed by the insertion of the Rieske iron-sulfur protein, a process for which BCS
Externí odkaz:
https://doaj.org/article/4f5b4fca633f4964a3749feb2ec9be5e
Publikováno v:
New Phytologist, 2016 Jul 01. 211(2), 554-568.
Externí odkaz:
https://www.jstor.org/stable/newphytologist.211.2.554
Publikováno v:
Antibiotics, Vol 10, Iss 6, p 723 (2021)
Blood-borne therapeutic phages and phage capsids increasingly reach therapeutic targets as they acquire more persistence, i.e., become more resistant to non-targeted removal from blood. Pathogenic bacteria are targets during classical phage therapy.
Externí odkaz:
https://doaj.org/article/16ea20f81a404bdb956803c6d692eb90