Výsledky vyhledávání - "nanosekundni pulzi"

Efficient gene transfection by electroporation—in vitro and in silico study of pulse parameters

Autor: Tjaša Potočnik, Shaurya Sachdev, Tamara Polajžer, Alenka Maček Lebar, Damijan Miklavčič

Publikováno v: Applied sciences, vol. 12, no. 16, 8237, 2022.
Applied Sciences; Volume 12; Issue 16; Pages: 8237

Gene electrotransfer (GET) is a widely used method for nucleic acids’ delivery into cells. We explored, evaluated, and demonstrated the potential use of different pulse durations for introducing plasmid DNA (pDNA) into cells in vitro and compared t

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a4f5b8bda8315380f3e3fe27708bc131
https://repozitorij.uni-lj.si/Dokument.php?id=171939&dn=

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Alternative pulse waveforms in electroporation-based technologies

Autor: Vižintin, Angelika

The aim of this study was to compare standard eight 100 µs pulses with alternative pulse waveforms (namely nanosecond and short high-frequency biphasic (H-FIRE) pulses) at different levels important for electroporation-based applications (extent of

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=od______3505::d88b7d542595b730a9ceea9f798c849a
https://repozitorij.uni-lj.si/IzpisGradiva.php?id=143584

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Efficiency and mechanisms of gene transfection by electroporation in mammalian cells in vitro

Autor: Potočnik, Tjaša

With the aim of better understanding and optimizing gene transfection by electroporation (GET) we tested the effect of different biological and electrical parameters on GET efficiency in vitro. Due to possibility that microenvironment in applications

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=od______3505::45caef6e95ce082887e3d17383c76eed
https://repozitorij.uni-lj.si/Dokument.php?id=166059&dn=

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Electroporation with nanosecond pulses and bleomycin or cisplatin results in efficient cell kill and low metal release from electrodes

Autor: Janez Ščančar, Stefan Marković, Damijan Miklavčič, Angelika Vižintin

Publikováno v: Bioelectrochemistry, vol. 140, no. 107798, 2021.

Nanosecond electric pulses have several potential advantages in electroporation-based procedures over the conventional micro- and millisecond pulses including low level of heating, reduced electrochemical reactions and reduced muscle contractions mak

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4611f467311e67c63332d40cc9b5b597
https://hdl.handle.net/20.500.12556/RUL-138666

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Pulse Duration Dependent Asymmetry in Molecular Transmembrane Transport Due to Electroporation in H9c2 Rat Cardiac Myoblast Cells In Vitro

Autor: Damijan Miklavčič, Tina Batista Napotnik

Publikováno v: Molecules, Vol 26, Iss 6571, p 6571 (2021)
Molecules, vol. 26, no. 21, 6571, 2021.
Molecules
Volume 26
Issue 21

Electroporation (EP) is one of the successful physical methods for intracellular drug delivery, which temporarily permeabilizes plasma membrane by exposing cells to electric pulses. Orientation of cells in electric field is important for electroporat

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d270c8b05556c4d4afd06411451d24f2
https://www.mdpi.com/1420-3049/26/21/6571

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UPORABA TEORETIČNIH MODELOV ELEKTROPORACIJE LIPIDNE MEMBRANE

Autor: Rems, Lea

V doktorski disertaciji smo pokazali, da lahko z numeričnimi modeli učinkovito prispevamo k eksperimentalnim raziskavam. Numerični modeli sicer ne morejo nadomestiti eksperimentov, lahko pa vodijo začetni razvoj eksperimentalnega protokola in nje

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=od______3505::1d497abebca71d816efa45d1d9d52faf
https://repozitorij.uni-lj.si/Dokument.php?id=80670&dn=

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