Zobrazeno 1 - 10
of 26 730
pro vyhledávání: '"model organisms"'
Autor:
Elisa Boscari, Stefano Dalle Palle, Nicola Vitulo, Annalisa Scapolatiello, Luca Schiavon, Alessia Cariani, Chiara Papetti, Lorenzo Zane, Ilaria Anna Maria Marino, Leonardo Congiu
Publikováno v:
Scientific Reports, Vol 14, Iss 1, Pp 1-14 (2024)
Abstract The study of species groups in which the presence of interspecific hybridization or introgression phenomena is known or suspected involves analysing shared bi-parentally inherited molecular markers. Current methods are based on different cat
Externí odkaz:
https://doaj.org/article/53c475663d8a450dad097ceb33da44b4
Publikováno v:
Microplastics, Vol 3, Iss 2, Pp 322-338 (2024)
The availability of compostable plastic bags has increased greatly in the past few years, as it is perceived that this type of bags will be degraded after disposal. However, there are some knowledge gaps regarding the potential effects on the soil ec
Externí odkaz:
https://doaj.org/article/3c503e14ee7445149d39318e961032d7
Publikováno v:
Frontiers in Plant Science, Vol 15 (2024)
Externí odkaz:
https://doaj.org/article/624d73b2e0fa472d9a19d4de73187fd6
Autor:
Prerna Bali, Ivonne Lozano-Pope, Jonathan Hernandez, Monica V. Estrada, Christopher Benner, Marygorret Obonyo
Publikováno v:
STAR Protocols, Vol 5, Iss 3, Pp 103302- (2024)
Summary: Helicobacter-induced gastric cancer progresses very slowly, even in animal models, making it difficult to study. Here, we present a protocol to establish an accelerated murine model for Helicobacter-induced gastric cancer. We describe steps
Externí odkaz:
https://doaj.org/article/2bf983f2dfdb40ceae3751e7f96ed0d2
Autor:
Raphael Pineau, Pierre Jean Le Reste, Tony Avril, Ulrich Jarry, Eric Chevet, Diana Pelizzari-Raymundo
Publikováno v:
STAR Protocols, Vol 5, Iss 3, Pp 103304- (2024)
Summary: In cancer research, murine models play a crucial role as highly valuable preclinical tools. Here, we present a protocol to generate a murine model of glioblastoma through the direct intracranial injection of tumor cells. We describe steps fo
Externí odkaz:
https://doaj.org/article/d6c8208a6d204dbaa5b17d57b18891d6
Autor:
Anxo Martinez-Ordoñez, Hiroto Kinoshita, Tania Cid-Diaz, Angeles Duran, Marta Osrodek, Maria T. Diaz-Meco, Jorge Moscat
Publikováno v:
STAR Protocols, Vol 5, Iss 3, Pp 103303- (2024)
Summary: Applying Opal multiplex immunofluorescence (OMI) to characterize intestinal tissues of genetically engineered mouse models provides an excellent tool for studying complex processes. However, detecting appropriate signals from multiple target
Externí odkaz:
https://doaj.org/article/a7f238d9bb394ea9b94266059e852c5d
Autor:
Javier Negueruela, Valerie Vandenbempt, Stephanie Talamantes, Francisco Ribeiro-Costa, Mariana Nunes, André Dias, Mayank Bansal, Esteban N. Gurzov
Publikováno v:
STAR Protocols, Vol 5, Iss 3, Pp 103297- (2024)
Summary: Gene editing of human pluripotent stem cells is a promising approach for developing targeted gene therapies for metabolic diseases. Here, we present a protocol for generating a CRISPR-Cas12a gene knockout of protein tyrosine phosphatases in
Externí odkaz:
https://doaj.org/article/c8a881473ccd4570afc4e34c60812a51
Autor:
Tiina Tamm, Arnold Kristjuhan
Publikováno v:
STAR Protocols, Vol 5, Iss 3, Pp 103282- (2024)
Summary: Isolation of amplifiable genomic DNA is a prerequisite for the implementation of PCR-based techniques. Here we present a protocol for isolating the genomic DNA from a variety of wild yeast species. This can be completed in approximately 1 h
Externí odkaz:
https://doaj.org/article/ba1eacc00ef144b4a5931bc3739e3928
Autor:
Zhijun Zhou, Jingxuan Yang, Mingyang Liu, Yu Ren, Xiuhui Shi, Yang Cai, Alex X. Arreola, Yi-Ping Li, Yuqing Zhang, Min Li
Publikováno v:
STAR Protocols, Vol 5, Iss 3, Pp 103281- (2024)
Summary: Cancer cachexia mouse models are needed to recapitulate the clinical features of patients with cachexia. Here, we present a protocol for the establishment and evaluation of cancer cachexia mouse models. We delineate the steps in preparing tu
Externí odkaz:
https://doaj.org/article/83ccedb77ed24acaa2218a37259ddec0
Publikováno v:
STAR Protocols, Vol 5, Iss 3, Pp 103184- (2024)
Summary: Generating a transgene with a reporter inserted into the genome helps us study endogenous gene expression patterns in model organisms. Here, using Drosophila melanogaster, we present a protocol for generating a P2A-Gal4 insertion through CRI
Externí odkaz:
https://doaj.org/article/1d382ad54e044e0fac60caf550410c80