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MicroPOTS Analysis of Barrett's Esophageal Cell Line Models Identifies Proteomic Changes after Physiologic and Radiation Stress

Autor: Ted R. Hupp, Borek Vojtesek, Tongjie Wang, Irena Dapic, Ying Zhu, Mowei Zhou, Ryan T. Kelly, David R. Goodlett, Javier A. Alfaro, Naomi Uwugiaren, Robert O'Neill, Ashita Singh, Kenneth Weke, Sarah M. Williams

Publikováno v: Journal of Proteome Research
Weke, K, Singh, A, Uwugiaren, N, Alfaro, J A, Wang, T, Hupp, T R, O'Neill, R, Vojtesek, B, R. Goodlett, D, M. Williams, S, Zhou, M, T. Kelly, R, Zhu, Y & Dapic, I 2021, ' MicroPOTS analysis of Barrett’s oesophageal cell line models identifies proteomic changes after physiologic and radiation stress ', Journal Of Proteome Research . https://doi.org/10.1021/acs.jproteome.0c00629

Moving from macroscale preparative systems in proteomics to micro- and nanotechnologies offers researchers the ability to deeply profile smaller numbers of cells that are more likely to be encountered in clinical settings. Herein a recently developed

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1c7e448a5533ceff33226e6663e15d79
https://pubmed.ncbi.nlm.nih.gov/33491460

Overall protocol for MicroPOTS LCMS top down proteomics of kidney tissue sections v1

Autor: Mowei Zhou

This is the overall workflow for LCMS top down proteomics of kidney functional units from tissue sections using the MicroPOTS platform. The expected outcomes are proteoform identification and quantitation values from selected tissue functional units.

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::a6a7070b14a3445c932e09ef1e44a778
https://doi.org/10.17504/protocols.io.eq2lynm1qvx9/v1

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Coupling Microdroplet-Based Sample Preparation, Multiplexed Isobaric Labeling, and Nanoflow Peptide Fractionation for Deep Proteome Profiling of the Tissue Microenvironment

Autor: Veličković, Marija, Fillmore, Thomas L., Attah, Isaac Kwame, Posso, Camilo, Pino, James C., Zhao, Rui, Williams, Sarah M., Veličković, Dušan, Jacobs, Jon M., Burnum-Johnson, Kristin E., Zhu, Ying, Piehowski, Paul D.

Publikováno v: Analytical Chemistry; August 2024, Vol. 96 Issue: 32 p12973-12982, 10p