Zobrazeno 1 - 10
of 13
pro vyhledávání: '"methods [Single-Cell Analysis]"'
Publikováno v:
Trends in molecular medicine 27(1), 47-59 (2021). doi:10.1016/j.molmed.2020.09.001
Microglia have long been considered a homogenous cell population that uniformly responds to extrinsic factors. Here, we describe how the recent development of single-cell technologies has revealed the heterogeneity of both human and mouse microglia a
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::94a22c990527363f3ff877fc2279fc5b
https://doi.org/10.1016/j.molmed.2020.09.001
https://doi.org/10.1016/j.molmed.2020.09.001
Autor:
Böttcher, C., van der Poel, M., Fernández-Zapata, C., Schlickeiser, S., Leman, J.K.H., Hsiao, C.-C., Mizee, M.R., Adelia, Vincenten, M.C.J., Kunkel, D., Huitinga, I., Hamann, J., Priller, J.
Publikováno v:
Acta Neuropathologica Communications, Vol 8, Iss 1, Pp 1-18 (2020)
Acta Neuropathologica Communications
Acta Neuropathologica Communications 8(1), 136 (2020). doi:10.1186/s40478-020-01010-8
Acta neuropathologica communications, 8(1). BioMed Central
Acta neuropathologica communications, 8(1):136. BioMed Central
Acta Neuropathologica Communications, 8:136. BioMed Central
Acta Neuropathologica Communications
Acta Neuropathologica Communications 8(1), 136 (2020). doi:10.1186/s40478-020-01010-8
Acta neuropathologica communications, 8(1). BioMed Central
Acta neuropathologica communications, 8(1):136. BioMed Central
Acta Neuropathologica Communications, 8:136. BioMed Central
Myeloid cells contribute to inflammation and demyelination in the early stages of multiple sclerosis (MS), but it is still unclear to what extent these cells are involved in active lesion formation in progressive MS (PMS). Here, we have harnessed the
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::827a2cd3238f9a9c901e6fcd08e3b1f0
http://link.springer.com/article/10.1186/s40478-020-01010-8
http://link.springer.com/article/10.1186/s40478-020-01010-8
Publikováno v:
STAR Protocols
STAR Protocols 1(1), 100042 (2020). doi:10.1016/j.xpro.2020.100042
STAR Protocols, Vol 1, Iss 1, Pp 100042-(2020)
STAR Protocols 1(1), 100042 (2020). doi:10.1016/j.xpro.2020.100042
STAR Protocols, Vol 1, Iss 1, Pp 100042-(2020)
SUMMARY Single-cell sequencing (sc-Seq) is a powerful tool to investigate the molecular signatures of cell types in a complex mixture of cells. A critical step in sc-Seq is preparing a single-cell suspension with a high number of viable cells. Here,
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f2e0acc06d6d5fdfb294cc6ca0f4b5de
https://pubmed.ncbi.nlm.nih.gov/32835293
https://pubmed.ncbi.nlm.nih.gov/32835293
Publikováno v:
Murgia, M, Tan, J, Geyer, P E, Doll, S, Mann, M & Klopstock, T 2019, ' Proteomics of Cytochrome c Oxidase-Negative versus-Positive Muscle Fiber Sections in Mitochondrial Myopathy ', Cell Reports, vol. 29, no. 12, pp. 3825-3834 . https://doi.org/10.1016/j.celrep.2019.11.055
Cell Reports
Cell reports 29(12), 3825-3834.e4 (2019). doi:10.1016/j.celrep.2019.11.055
CELL REPORTS
Cell Reports, Vol 29, Iss 12, Pp 3825-3834.e4 (2019)
Cell Reports
Cell reports 29(12), 3825-3834.e4 (2019). doi:10.1016/j.celrep.2019.11.055
CELL REPORTS
Cell Reports, Vol 29, Iss 12, Pp 3825-3834.e4 (2019)
Summary: The mosaic distribution of cytochrome c oxidase+ (COX+) and COX− muscle fibers in mitochondrial disorders allows the sampling of fibers with compensated and decompensated mitochondrial function from the same individual. We apply laser capt
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::def9685ca456bda15a630a6591711992
http://hdl.handle.net/11577/3317481
http://hdl.handle.net/11577/3317481
Autor:
Simone Picelli
Publikováno v:
Methods in Molecular Biology ISBN: 9781493992393
New York, NY : Springer New York, Methods in Molecular Biology 1979, 25-44 (2019). doi:10.1007/978-1-4939-9240-9_3
Single Cell Methods / Proserpio, Valentina (Editor) ; New York, NY : Springer New York, 2019, Chapter 3 ; ISSN: 1064-3745=1940-6029 ; ISBN: 978-1-4939-9239-3=978-1-4939-9240-9 ; doi:10.1007/978-1-4939-9240-9
New York, NY : Springer New York, Methods in Molecular Biology 1979, 25-44 (2019). doi:10.1007/978-1-4939-9240-9_3
Single Cell Methods / Proserpio, Valentina (Editor) ; New York, NY : Springer New York, 2019, Chapter 3 ; ISSN: 1064-3745=1940-6029 ; ISBN: 978-1-4939-9239-3=978-1-4939-9240-9 ; doi:10.1007/978-1-4939-9240-9
In the last few years single-cell RNA sequencing (scRNA-seq) has enabled the investigation of cellular heterogeneity at the transcriptional level, the characterization of rare cell types as well as the detailed analysis of the stochastic nature of ge
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d2a78a845d87dd65f096254eb1796a9d
https://doi.org/10.1007/978-1-4939-9240-9_3
https://doi.org/10.1007/978-1-4939-9240-9_3
Publikováno v:
Methods in Molecular Biology ISBN: 9781493992393
New York, NY : Springer New York, Methods in Molecular Biology 1979, 433-455 (2019). doi:10.1007/978-1-4939-9240-9_26
Single Cell Methods / Proserpio, Valentina (Editor) ; New York, NY : Springer New York, 2019, Chapter 26 ; ISSN: 1064-3745=1940-6029 ; ISBN: 978-1-4939-9239-3=978-1-4939-9240-9 ; doi:10.1007/978-1-4939-9240-9
New York, NY : Springer New York, Methods in Molecular Biology 1979, 433-455 (2019). doi:10.1007/978-1-4939-9240-9_26
Single Cell Methods / Proserpio, Valentina (Editor) ; New York, NY : Springer New York, 2019, Chapter 26 ; ISSN: 1064-3745=1940-6029 ; ISBN: 978-1-4939-9239-3=978-1-4939-9240-9 ; doi:10.1007/978-1-4939-9240-9
The recent technological developments in the field of single-cell RNA-Seq enable us to assay the transcriptome of up to a million single cells in parallel. However, the analyses of such big datasets present a major challenge. During the last decade,
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d2c38f3221de1804f85dce49b45dfcfc
https://doi.org/10.1007/978-1-4939-9240-9_26
https://doi.org/10.1007/978-1-4939-9240-9_26
Autor:
Böttcher, C., Fernández-Zapata, C., Schlickeiser, S., Kunkel, D., Schulz, A.R., Mei, H.E., Weidinger, C., Gieß, R.M., Asseyer, S., Siegmund, B., Paul, F., Ruprecht, K., Priller, J.
Publikováno v:
Scientific reports 9(1), 19471 (2019). doi:10.1038/s41598-019-55852-x
Scientific Reports, Vol 9, Iss 1, Pp 1-14 (2019)
Scientific Reports
Scientific Reports, Vol 9, Iss 1, Pp 1-14 (2019)
Scientific Reports
Multiple sclerosis (MS) is an inflammatory demyelinating and neurodegenerative disease of the central nervous system (CNS). Studies in rodent models demonstrated an association of CNS-infiltrating monocyte-derived macrophages with disease severity. H
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cc00df5264e9b62af103180cdcc39ab1
Autor:
Andreas Dahl, Prabesh Bhattarai, Andreas Petzold, Susanne Reinhardt, Caghan Kizil, Mehmet Ilyas Cosacak, Yixin Zhang
Publikováno v:
Cell reports 27(4), 1307-1318.e3 (2019). doi:10.1016/j.celrep.2019.03.090
The neural stem cell (NSC) reservoir can be harnessed for stem cell-based regenerative therapies. Zebrafish remarkably regenerate their brain by inducing NSC plasticity in a Amyloid-β-42 (Aβ42)-induced experimental Alzheimer's disease (AD) model. I
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9d0697dcfe5d0d0254c22de0ce316e27
https://pub.dzne.de/record/140652
https://pub.dzne.de/record/140652
Autor:
Katrin Gehring, Lu Liu, Fumere Usifo, Mikael Simons, Tuğberk Kaya, Ozgun Gokce, Buket Bulut, Hao Ji, Simon Besson-Girard
Publikováno v:
STAR Protocols 2(2), 100590-(2021). doi:10.1016/j.xpro.2021.100590
STAR Protocols, Vol 2, Iss 2, Pp 100590-(2021)
STAR Protocols
STAR Protocols, Vol 2, Iss 2, Pp 100590-(2021)
STAR Protocols
Summary Single-cell RNA sequencing (scRNA-seq) provides the transcriptome of individual cells and addresses previously intractable problems including the central nervous system’s transcriptional responses during health and disease. However, dissoci
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::98bddc5ed01d56f4daf862963ca139d2
https://doi.org/10.1016/j.xpro.2021.100590
https://doi.org/10.1016/j.xpro.2021.100590
Autor:
Kristian Händler, Matthias Becker, Lorenzo Bonaguro, Joachim L. Schultze, Jonas Schulte-Schrepping, Elena De Domenico
Publikováno v:
STAR Protocols
STAR Protocols, Vol 1, Iss 3, Pp 100233-(2020)
STAR Protocols 1(3), 100233 (2020). doi:10.1016/j.xpro.2020.100233
STAR Protocols, Vol 1, Iss 3, Pp 100233-(2020)
STAR Protocols 1(3), 100233 (2020). doi:10.1016/j.xpro.2020.100233
Summary In December 2019, a new coronavirus, SARS-CoV-2, which causes the respiratory illness that led to the COVID-19 pandemic, was reported. In the face of such a new pathogen, special precautions must be taken to examine potentially infectious mat
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::55916c2f7c6ef86601ccbc9c85d8818a
https://doi.org/10.1016/j.xpro.2020.100233
https://doi.org/10.1016/j.xpro.2020.100233