Zobrazeno 1 - 9
of 9
pro vyhledávání: '"methods [Cell Culture Techniques]"'
Autor:
Eldem Sadikoglou, Joachim Täger, Manmeet-Sakshi Bedi, Noemia Fernandes, Stefanie Bröer, Peter Heutink, Salvador Rodriguez-Nieto, Ashutosh Dhingra, Patrizia Rizzu, Elisangela Bressan, Melissa Castillo-Lizardo
Publikováno v:
JoVE journal Biology(162), 61525 (2020). doi:10.3791/61525
Manual culture and differentiation protocols for human induced pluripotent stem cells (hiPSC) are difficult to standardize, show high variability and are prone to spontaneous differentiation into unwanted cell types. The methods are labor-intensive a
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::522281cdee11ab0c7713c21844c76581
https://doi.org/10.3791/61525
https://doi.org/10.3791/61525
Autor:
Wen Ping Kuo-Elsner, Chaitali Mukherjee, Christina Müller, Mikael Simons, Marie-Theres Weil, Isabelle Schäfer, Giselheid Schulz-Ëberlin
Publikováno v:
Oligodendrocytes ISBN: 9781493990702
New York, NY : Springer New York, Methods in Molecular Biology 1936, 79-95 (2019). doi:10.1007/978-1-4939-9072-6_5
Oligodendrocytes / Lyons, David A. (Editor) ; New York, NY : Springer New York, 2019, Chapter 5 ; ISSN: 1064-3745=1940-6029 ; ISBN: 978-1-4939-9070-2=978-1-4939-9072-6 ; doi:10.1007/978-1-4939-9072-6
New York, NY : Springer New York, Methods in Molecular Biology 1936, 79-95 (2019). doi:10.1007/978-1-4939-9072-6_5
Oligodendrocytes / Lyons, David A. (Editor) ; New York, NY : Springer New York, 2019, Chapter 5 ; ISSN: 1064-3745=1940-6029 ; ISBN: 978-1-4939-9070-2=978-1-4939-9072-6 ; doi:10.1007/978-1-4939-9072-6
Primary cultures of brain-derived rodent cells are widely used to study molecular and cellular mechanisms in neurobiology. In this chapter, we describe methods of purifying and culturing oligodendroglial cells from mouse perinatal brains. In addition
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::aa97981685e7b0c306ffa185adda4c2d
https://doi.org/10.1007/978-1-4939-9072-6_5
https://doi.org/10.1007/978-1-4939-9072-6_5
Publikováno v:
Stem cell research 34, 101378 (2019). doi:10.1016/j.scr.2018.101378
Stem Cell Research, Vol 34, Iss, Pp-(2019)
Stem Cell Research, Vol 34, Iss, Pp-(2019)
STUB1/CHIP is a central component of cellular protein homeostasis and interacts with key proteins involved in the pathogenesis of many neurodegenerative diseases. Missense and truncating mutations in STUB1 lead to SCAR16. For ideal in vitro disease m
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid_dedup__::40a995ea112a51750bc1ba286050faff
https://pubmed.ncbi.nlm.nih.gov/30605842
https://pubmed.ncbi.nlm.nih.gov/30605842
Autor:
Carsten Werner, Steffen Vogler, Marcus Binner, Silvana Prokoph, Gerd Kempermann, Mikhail V. Tsurkan, Uwe Freudenberg
Publikováno v:
Scientific reports 8(1), 8433 (2018). doi:10.1038/s41598-018-26417-1
Scientific Reports, Vol 8, Iss 1, Pp 1-12 (2018)
Scientific Reports
Scientific Reports, Vol 8, Iss 1, Pp 1-12 (2018)
Scientific Reports
Distinct micro-environmental properties have been reported to be essential for maintenance of neural precursor cells (NPCs) within the adult brain. Due to high complexity and technical limitations, the natural niche can barely be studied systematical
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::61de6ea35734be8ab459fe3f678423c1
https://doi.org/10.1038/s41598-018-26417-1
https://doi.org/10.1038/s41598-018-26417-1
Publikováno v:
New York, NY : Springer New York, Methods in Molecular Biology 1784, 35-49 (2018). doi:10.1007/978-1-4939-7837-3_4
Macrophages / Rousselet, Germain (Editor) ; New York, NY : Springer New York, 2018, Chapter 4 ; ISSN: 1064-3745=1940-6029 ; ISBN: 978-1-4939-7836-6=978-1-4939-7837-3 ; doi:10.1007/978-1-4939-7837-3
Macrophages ISBN: 9781493978366
Macrophages / Rousselet, Germain (Editor) ; New York, NY : Springer New York, 2018, Chapter 4 ; ISSN: 1064-3745=1940-6029 ; ISBN: 978-1-4939-7836-6=978-1-4939-7837-3 ; doi:10.1007/978-1-4939-7837-3
Macrophages ISBN: 9781493978366
Macrophages are specialized phagocytes that display a variety of important functions for the host immune system. They are particularly important for the recognition of exogenous and endogenous danger signals, forming the defensive front line as part
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::26b8577ceec89a38a66bbaf7f079cf1e
Autor:
Hans Zempel, Eva-Maria Mandelkow
Publikováno v:
Methods in Molecular Biology ISBN: 9781493965960
New York, NY : Springer New York, Methods in Molecular Biology 1523, 327-334 (2017). doi:10.1007/978-1-4939-6598-4_20
Tau Protein / Smet-Nocca, Caroline (Editor) ; New York, NY : Springer New York, 2017, Chapter 20 ; ISSN: 1064-3745=1940-6029 ; ISBN: 978-1-4939-6596-0=978-1-4939-6598-4 ; doi:10.1007/978-1-4939-6598-4
New York, NY : Springer New York, Methods in Molecular Biology 1523, 327-334 (2017). doi:10.1007/978-1-4939-6598-4_20
Tau Protein / Smet-Nocca, Caroline (Editor) ; New York, NY : Springer New York, 2017, Chapter 20 ; ISSN: 1064-3745=1940-6029 ; ISBN: 978-1-4939-6596-0=978-1-4939-6598-4 ; doi:10.1007/978-1-4939-6598-4
Primary neurons have proved to be an invaluable tool for the investigation of Tau in the context of neuronal development and neurodegeneration. Culturing neurons usually is time consuming and requires multiple feeding steps and media exchanges, and e
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2031c2266698277ad5167de992cc5f9c
https://doi.org/10.1007/978-1-4939-6598-4_20
https://doi.org/10.1007/978-1-4939-6598-4_20
Autor:
Gorris, Raphaela, Fischer, Julia, Brüstle, Oliver, Erwes, Kim Lina, Kesavan, Jaideep, Peterson, Daniel A, Alexander, Michael, Nöthen, Markus M, Peitz, Michael, Quandel, Tamara, Karus, Michael
Publikováno v:
Glia 63(12), 2152-2167 (2015). doi:10.1002/glia.22882
Neural precursor cells (NPCs) derived from human pluripotent stem cells (hPSCs) represent an attractive tool for the in vitro generation of various neural cell types. However, the developmentally early NPCs emerging during hPSC differentiation typica
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid_dedup__::4dcaa6969dbd82e7367fc480320e9509
https://pubmed.ncbi.nlm.nih.gov/26123132
https://pubmed.ncbi.nlm.nih.gov/26123132
Autor:
Ina Nuesslein, Stefan Kißenkötter, Alexander Jarosch, Alexander Storch, Anne K. Meyer, Katja Schurig
Publikováno v:
Brain research 1474, 8-18 (2012). doi:10.1016/j.brainres.2012.07.034
Stem cells have one major advantage over primary cells for regenerative therapies in neurodegenerative diseases. They are able to self-renew making sufficient quantities of cells available for transplantation. Embryonic stem cells and fetal neural pr
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a15a612660b3bf4dc60f4037d70f8ea5
https://pubmed.ncbi.nlm.nih.gov/22842082
https://pubmed.ncbi.nlm.nih.gov/22842082
Publikováno v:
Biotechnology progress 19, 1562-1568 (2003). doi:10.1021/bp034016f
The in vitro assembly of neuronal networks with control over cell position and connectivity is a fascinating approach not only for topics in basic neuroscience research but also in diverse applications such as biosensors and tissue engineering. We gr
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c48ccc881fb01e206d5779a2fffc9096
https://juser.fz-juelich.de/record/29298
https://juser.fz-juelich.de/record/29298