Overexpression of the ABC Transporter BmrA Within Intracellular Caveolae in Escherichia coli

Autor: Alexia Gobet, Christine Jaxel, Sandrine Magnard, Manuel Garrigos, Stéphane Orlowski, Nadège Jamin, Pierre Falson, Vincent Chaptal

Publikováno v: Methods in Molecular Biology ISBN: 9781071623671
Heterologous Expression of Membrane Proteins
Heterologous Expression of Membrane Proteins, 2507, Springer US, pp.79-89, 2022, Methods in Molecular Biology, ⟨10.1007/978-1-0716-2368-8_5⟩

International audience; We described here the overproduction and oriented membrane insertion of membrane protein inside intracellular vesicles named heterologous caveolae within E. coli. The method is described with BmrA, a multidrug efflux pump from

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::800e8d36c3deabe6bebc074035f222bc
https://doi.org/10.1007/978-1-0716-2368-8_5

Ectopic Neo-Formed Intracellular Membranes in Escherichia coli: A Response to Membrane Protein-Induced Stress Involving Membrane Curvature and Domains

Autor: Stéphane Orlowski, Nadège Jamin, Christine Jaxel, Manuel Garrigos, Annie Frelet-Barrand

Publikováno v: Biomolecules
Biomolecules, MDPI, 2018, 8 (3), pp.88. ⟨10.3390/biom8030088⟩
Biomolecules, Vol 8, Iss 3, p 88 (2018)
Biomolecules, 2018, 8 (3), pp.88. ⟨10.3390/biom8030088⟩

International audience; Bacterial cytoplasmic membrane stress induced by the overexpression of membrane proteins at high levels can lead to formation of ectopic intracellular membranes. In this review, we report the various observations of such membr

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::18b238030f18432ccd1aa2d7e49198b6
https://hal.archives-ouvertes.fr/hal-02410600

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Physiological Adaptation of the Bacterium Lactococcus lactis in Response to the Production of Human CFTR

Publikováno v: Molecular & Cellular Proteomics. 10(7):1-16

Biochemical and biophysical characterization of CFTR (the cystic fibrosis transmembrane conductance regulator) is thwarted by difficulties to obtain sufficient quantities of correctly folded and functional protein. Here we have produced human CFTR in

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=dris___01423::69b19693e6bc7525a226fdb141944db3
https://doi.org/10.1074/mcp.M000052-MCP200

Manipulating the genetic code for membrane protein production : What have we learnt so far?

Autor: Arne Elofsson, Sara Light, Morten H. H. Nørholm, Gunnar von Heijne, Daniel O. Daley, Minttu T. Virkki

With synthetic gene services, molecular cloning is as easy as ordering a pizza. However choosing the right RNA code for efficient protein production is less straightforward, more akin to deciding on the pizza toppings. The possibility to choose synon

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b324c9c0d4de21b5a8032d043840e5e3
http://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-66922