Determining antioxidant activities of lactobacilli by cellular antioxidant assay in mammal cells

Autor: Jiali Xing, Gang Wang, Qiuxiang Zhang, Xiaoming Liu, Boxing Yin, Dongsheng Fang, Jianxin Zhao, Hao Zhang, Yong Q. Chen, Wei Chen

Publikováno v: Journal of Functional Foods, Vol 19, Iss , Pp 554-562 (2015)

Among the beneficial effects of Lactobacillus spp., the common mechanism underlying health protection is believed to be related to antioxidant activity. The antioxidant activities of cell-free suspension (CFS) and strain suspension (SS) of 13 Lactoba

Externí odkaz: https://doaj.org/article/e2d55bbcf1bc425ea2d4690cbcd783cb

Temporal and Spatial Epigenome Editing Allows Precise Gene Regulation in Mammalian Cells

Autor: Natasha Lopes Fischer, Rashad Mammadov, Stefan Bekiranov, Hayrunnisa Unlu, Turan Tufan, Cem Kuscu, Mazhar Adli, Sevki Arslan, Masato T. Kanemaki, Agnes Czikora

Publikováno v: Journal of molecular biology. 431(1)

Cell-type specific gene expression programs are tightly linked to epigenetic modifications on DNA and histone proteins. Here, we used a novel CRISPR-based epigenome editing approach to control gene expression spatially and temporally. We show that ta

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3162e4123e128e44c4adb6a91a18b072
https://pubmed.ncbi.nlm.nih.gov/30098338

Towards the development of a surface plasmon resonance assay to evaluate the glycosylation pattern of monoclonal antibodies using the extracellular domains of CD16a and CD64

Autor: Gregory De Crescenzo, July Dorion-Thibaudeau, Erika Lattová, Céline Raymond, Hélène Perreault, Yves Durocher

Publikováno v: Journal of Immunological Methods. 408:24-34

We here report the production and purification of the extracellular domains of two Fcγ receptors, namely CD16a and CD64, by transient transfection in mammalian cells. The use of these two receptor ectodomains for the development of quantitative assa

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::088d8e441300a073e33f3d67a60e3861
https://doi.org/10.1016/j.jim.2014.04.010

Che1/AATF interacts with subunits of the histone acetyltransferase core module of SAGA complexes

Autor: Ikbal Cansu Baris, Sevil Zencir, Zeki Topcu, Muge Senarisoy, Melanie J. Dobson, Gizem Caliskan, Imre Boros, Ferhan Ayaydin

Publikováno v: PLoS ONE
PLoS ONE, Vol 12, Iss 12, p e0189193 (2017)

WOS: 000417698200025
PubMed ID: 29232376
General Control Non-derepressible 5 (GCN5) and Alteration/Deficiency in Activation 2 and 3 proteins (ADA2 and ADA3, respectively) are subunits of the Histone AcetylTransferase (HAT) module of SAGA-an

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::fa09b7f075b8a6b906995e86eee21f0e
https://hdl.handle.net/11499/8840

RSUME, a Small RWD-Containing Protein, Enhances SUMO Conjugation and Stabilizes HIF-1α during Hypoxia

Autor: Susana Silberstein, Eduardo Arzt, Florian Holsboer, Günter K. Stalla, Alberto Carbia-Nagashima, Juan Gerez, Carolina Perez-Castro, Marcelo Paez-Pereda

Publikováno v: Cell 2007;131(2):309-323
Biblioteca Digital (UBA-FCEN)
Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
instacron:UBA-FCEN

SUMO conjugation to proteins is involved in the regulation of diverse cellular functions. We have identified a protein, RWD-containing sumoylation enhancer (RSUME), that enhances overall SUMO-1, -2, and -3 conjugation by interacting with the SUMO con

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9219324c09a7cb2baf0042d7f8fc926e
https://doi.org/10.1016/j.cell.2007.07.044