Zobrazeno 1 - 10
of 273
pro vyhledávání: '"low biomass"'
Publikováno v:
Frontiers in Microbiology, Vol 15 (2024)
DNA extraction yield from drinking water distribution systems and premise plumbing is a key metric for any downstream analysis such as 16S amplicon or metagenomics sequencing. This research aimed to optimize DNA yield from low-biomass (chlorinated) r
Externí odkaz:
https://doaj.org/article/103cbdd51d7e4745a781692cbfdf6c44
Autor:
Sophie A. Simon, Katharina Schmidt, Lea Griesdorn, André R. Soares, Till L. V. Bornemann, Alexander J. Probst
Publikováno v:
BMC Genomics, Vol 24, Iss 1, Pp 1-11 (2023)
Abstract Background While genome-resolved metagenomics has revolutionized our understanding of microbial and genetic diversity in environmental samples, assemblies of short-reads often result in incomplete and/or highly fragmented metagenome-assemble
Externí odkaz:
https://doaj.org/article/0b4b70b4993a4e08b46a6fd814cfde88
Autor:
Claudia Hülpüsch, Luise Rauer, Thomas Nussbaumer, Vera Schwierzeck, Madhumita Bhattacharyya, Veronika Erhart, Claudia Traidl-Hoffmann, Matthias Reiger, Avidan U. Neumann
Publikováno v:
BMC Biology, Vol 21, Iss 1, Pp 1-20 (2023)
Abstract Background Microbiome analysis is becoming a standard component in many scientific studies, but also requires extensive quality control of the 16S rRNA gene sequencing data prior to analysis. In particular, when investigating low-biomass mic
Externí odkaz:
https://doaj.org/article/98cd81221ec34035a47f458a7dbb6717
Publikováno v:
Veterinary Research, Vol 54, Iss 1, Pp 1-13 (2023)
Abstract Changes in the milk microbiota during the course of mastitis are due to the nature of a sporadic occurring disease difficult to study. In this study we experimentally induced mastitis by infusion of Escherichia coli endotoxins in one udder q
Externí odkaz:
https://doaj.org/article/4476ea5f5cca496bae65f2cba78916df
Autor:
Jiaxian Shen, Alexander G. McFarland, Ryan A. Blaustein, Laura J. Rose, K. Allison Perry-Dow, Anahid A. Moghadam, Mary K. Hayden, Vincent B. Young, Erica M. Hartmann
Publikováno v:
Microbiome, Vol 10, Iss 1, Pp 1-18 (2022)
Abstract Background Effective surveillance of microbial communities in the healthcare environment is increasingly important in infection prevention. Metagenomics-based techniques are promising due to their untargeted nature but are currently challeng
Externí odkaz:
https://doaj.org/article/a92d5d5a63874e5185bd4bc240ae5690
Publikováno v:
Microbiology Spectrum, Vol 11, Iss 4 (2023)
ABSTRACT Concrete hosts a small but diverse microbiome that changes over time. Shotgun metagenomic sequencing would enable assessment of both the diversity and function of the microbial community in concrete, but a number of unique challenges make th
Externí odkaz:
https://doaj.org/article/ccbe1175da4240aa99dc31b1b290e6cc
Autor:
Aleksandra I. Krawczyk, Lisa Röttjers, Manoj Fonville, Katshuisa Takumi, Willem Takken, Karoline Faust, Hein Sprong
Publikováno v:
Microbiome, Vol 10, Iss 1, Pp 1-15 (2022)
Abstract Background Ixodes ricinus ticks vector pathogens that cause serious health concerns. Like in other arthropods, the microbiome may affect the tick’s biology, with consequences for pathogen transmission. Here, we explored the bacterial commu
Externí odkaz:
https://doaj.org/article/9a12fccd36fe4698a805157f54a353ed
Publikováno v:
MicrobiologyOpen, Vol 12, Iss 3, Pp n/a-n/a (2023)
Abstract The simultaneous extraction of intracellular DNA (iDNA) and extracellular DNA (eDNA) can help to separate the living in situ community (represented by iDNA) from background DNA that originated both from past communities and from allochthonou
Externí odkaz:
https://doaj.org/article/c6076493a31b4761a422e4241124477f
Publikováno v:
BMC Microbiology, Vol 21, Iss 1, Pp 1-14 (2021)
Abstract Background One limiting factor of short amplicon 16S rRNA gene sequencing approaches is the use of low DNA amounts in the amplicon generation step. Especially for low-biomass samples, insufficient or even commonly undetectable DNA amounts ca
Externí odkaz:
https://doaj.org/article/06df479deb10444a9714f74b4607114e
Autor:
Suk Yee Lam, Athanasia Ioannou, Prokopis Konstanti, Thijmen Visseren, Michail Doukas, Maikel Petrus Peppelenbosch, Clara Belzer, Gwenny Manel Fuhler
Publikováno v:
BMC Microbiology, Vol 21, Iss 1, Pp 1-11 (2021)
Abstract Background Formalin-fixed paraffin embedded (FFPE) tissues may provide an exciting resource to study microbial associations in human disease, but the use of these low biomass specimens remains challenging. We aimed to reduce unintentional ba
Externí odkaz:
https://doaj.org/article/4ba074a5e2fe45e38aca55cf36784862